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Biomedical subjects

F Rousselet

Publications and source records attributed to F Rousselet.

At least 73 records · Page 4Linked to original sources
PubMed1976

[Proposal of a new procedure for the exploration of hepatocellular function. The trimethoxybenzene test (TMB)].

The authors propose a new method of exploration of liver cell function based on the study of demethylation of a rapidly assimilated atoxic compound, trimethoxybenzene (TMB) in dimethoxy-hydroxybenzene (DMHB). Whereas in normal subjects, the urinary excretion of the metabolites was rapid and marked, 180 mg within 3 hours after taking a 400 mg dose, in patients with liver cell failure, the excretion of DMHB was considerably reduced, 80 to 95% in cirrhosis.

Benzene Derivatives↗
PubMed1976

[Study of the specificity of action of cholesterol oxygen oxidoreductase on various sterols and related substances].

The authors studied the action of oxygen cholesterol oxidoreductase (E.C. 1.1.3.6) extracted from Nocardia erythropolis on different sterols following simultaneously:--the disappearance of the substrate by chromatography on thin layers of silic acid, the formation of hydrogen peroxide by Röschlau's reaction, changes in the absorption spectrum between 220 and 320 nm. The results obtained show that sterol oxidase presents a broad spectrum of activity. It catalyses the transformation into ketone of the secondary alcohol group at the 3 beta position of sterols which possess in the C17 position a lateral chain of at least two carbon atoms. In the other hand, the reaction does not take place if there exists in the C5 position, an H in the cis position or if two hydrogen atoms linked to carbon 4 are substituted. Finally, only oxidation products derived from sterols possess a double bond situated in the 4-5 or 5-6 position presenting an absorption band between 240 and 250 nm.

Chromatography, Thin Layer↗
PubMed1975

[Coupled use of chromatography and acrylamide-agarose gel electrophoresis in the study of polymeric forms of paraproteins].

A study of the physical properties of paraprotein required until now, the use of fairly complicated techniques, such as analytical ultracentrifugation. The use of chromatography on acrylamide agar pearls and electrophoresis on indubiose plates, coupled with the classical techniques of electrophoresis and immunoelectrophoresis, permitted a more simple approach to these problems. The study of IgA paraproteins in several polymerised forms, is an example of the possibilities of this new method of investigation.

Chromatography↗
PubMed1975

[Strontium and calcium metabolism. Interaction of strontium and vitamin D].

Oral administration of strontium to calcium wellfed rats blocks the intestinal absorption of calcium. When high doses of vitamine D are given over long period, the inhibition of calcium intestinal absorption disapears. Under these conditions the absorption of strontium is increased. It is suggested that there is only one absorption mechanism for these two cations. An overdose of the vitamine D increases the renal elimination of strontium but under these conditions the plasma concentration of the strontium is unchanged. Vitamine D brings about the some action on the bone fixation of the strontium as it does on the bone fixation of calcium. The bone fixation is increased with low dosages. The bone fixation is decreased with high dosages.

Animals↗