[Polypoid-tubular metaplasia of the urinary bladder (so-called nephrogenic adenoma)].
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Cystoscopy↗
Biomedical subjects
F Theuring
Publications and source records attributed to F Theuring.
S-antigen and rod-opsin immunoreactions in midline brain neoplasms of transgenic mice: similarities to pineal cell tumors and certain medulloblastomas in man.
Transgenic mice expressing the large T-antigen of the simian virus 40 (SV 40) under the control of 1) the enhancer of Moloney murine sarcoma virus (MSV) and 2) the SV 40 promoter develop undifferentiated neuroectodermal tumors located in the midline of the dorsal brain surface, abnormalities in lens fiber differentiation and retinal dysplasia. In this study the brain neoplasms of six adult animals and the brain of one 11-day old mouse were examined by conventional histology and immunocytochemical demonstration of S-antigen, rod-opsin, neuron-specific enolase, neurofilaments (160 and 200 kDa) and glial fibrillary acidic protein. According to histologic criteria the neoplasms were characterized as "primitive" neuroectodermal tumors composed mainly of small cells with scanty and ill-defined cytoplasm. Neoplastic cells displaying immunoreactive S-antigen were found in five brain tumors; three of these tumors also contained a limited number of rod-opsin immunoreactive neoplastic cells. Some tumor cells had neurite-like processes containing immunoreactive neurofilament (200 kDa). No pathologic lesions were found in the brain of the 11-day old animal. Tumors in transgenic mice may resemble pineal cell tumors and a special subtype of medulloblastoma in man. These neoplasms contain S-antigen immunoreactive and also rod-opsin immunoreactive tumors cells in certain cases. The findings suggest that transgenic mice expressing the large T-antigen of SV 40 may become a valuable animal model for analysing the origin, histogenesis and development of primitive neuroectodermal tumors with photoreceptor-like features (pineal cell tumors and certain medulloblastomas).
Animals↗
[Autoradiographic determination of adrenal gland proliferation in SHR (spontaneously hypertensive rats)].
The histological pictures of the adrenal cortex of spontaneously hypertensive rats were investigated with autoradiographic and morphometric methods. No differences, between the tritiated thymidine index of SHR and controls were detected in the 3 cortex zones. In contrast to the normal fasciculated zone a decreased number of cells per area was shown in the zona fasciculata with hypertension depended findings.
Animals↗
[Physiologic regeneration of rat urothelium after pulse and continual labeling with 3H-thymidine at various ages].
We investigated the physiological regeneration and the growth fraction of the growth fraction of the urothel in pyelon, ureter and urinary bladder from rats. The rate physiological regeneration was assessed with a single intraperitoneal doses of 37 KBq 3H-Thymidin/g body weight and the growth fraction with multiple administrations of the same doses over the time of 4 days. The age of the rats ranged from 60, 100 and to 165 days. The labelling index in the urothel was highest at the age of 60 days and yielded a ratio of approximately 0.6% in ureter, in pyelon approximately 0.35% and in the urinary bladder approximately 0.1%. The highest level labelling index was found within the bladder in the medial region and the lowest in the top third. The size of growth fraction in the animals in age of 60 days demonstrated in ureter approximately 6%, in pyelon approximately 3% and in the urinary bladder approximately 1%. The growth fraction of urothel, e.g. ureter, demonstrated a large difference between the basal (approximately 10%) and suprabasal cells (approximately 3%) in animals with on age from 60 days.
Aging↗
[Proliferation kinetics in rat urothelium after unilateral nephrectomy and various doses of dibutylnitrosamine application--an example of the roles of a one kidney state on carcinogenesis].
We investigated the proliferation of urothel of the rat after unilateral nephrectomy and application of DBN with drinking water in various doses. We controlled the measurement of proliferation with 3H-thymidine. The important results of our experiments with DBN-application and unilateral nephrectomy were: 1. the tumour-incidence is higher and 2. the tumour-appear is earlier than in experiments with only DBN.
Animals↗
Tumorigenesis and eye abnormalities in transgenic mice expressing MSV-SV40 large T-antigen.
Transgenic mice which expressed SV40 large T-antigen under the control of the MSV enhancer and the SV40 promoter were generated. In animals containing an intact MSV enhancer, total lens cataracts and neuroectodermal brain tumors, originating in the pineal organ were observed. In contrast, 5' deletion of the MSV enhancer to a residual 53 bp resulted in a different spectrum of pathologies. Whilst lens cataracts still occurred, no brain tumors could be detected. Instead, fibrosarcomas and adenocarcinomas of the kidneys were induced. In addition, tumors of the endocrine pancreas were observed with both transgene constructs. We conclude that the MSV enhancer element is sufficient to direct the expression of the viral reporter gene to the lens and the pineal organ in transgenic mice. Deletion of the MSV enhancer correlates with the loss of DNA elements responsible for the pineal cell specific expression of SV40 large T-antigen.
Animals↗
[Systematic histologic urinary bladder mapping in primary urinary bladder cancers].
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Adult↗
[The systematic histologic elaboration of urinary bladders with different pathologic findings (urinary bladder carcinoma, tumor-free aging urinary bladders)].
We report our systematic histological examinations of 16 urinary bladders (11 bladders with cancer, 5 bladders was tumour-free). All residual tumour-free portions in bladder with multiple or solitary cancers demonstrated various precancerous variations of urothelium, not only near the tumours. In 1 urinary bladder among 5 from elder patients, we diagnosed a clinically undetected cancer. In a few cases, we correlated histological examinations with graphical demonstration (through a computer) of the exophytic tumour portions. Our results agree with individual therapy of urinary bladder cancer.
Aging↗
[Pathomorphologic contribution to the epidemiology of emergency medical conditions in advanced age].
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Adolescent↗
[Utriculus cysts--report of a rare manifestation].
It is reported on a rare partial intravesical utriculus cyst in a 72-year-old man. The particularities of the topography and the histological structure of the wall are presented and the diagnostical delimination compared with cysts of other pathogenesis are discussed. Cysts of the Müllerian Duct are deliminated from the utriculus cysts. In the present case of an intramural utriculus cyst a conservative approach (cyst punction) is indicated. A radical removal is impossible, in contrast to the extravesical utriculus cysts. The collapse of the cyst, the absence of subjective trouble, the non-inflammatory altered cyst wall, and the age of the deceased man indicate a favourable prognosis of the utriculus cysts.
Aged↗
[Reactions of tumorous urinary bladders following local and systemic therapy with cytostatic drugs].
In 22 patients with bladder cancer after local chemotherapy and 13 patients after systemic chemotherapy, respectively, multiple biopsy specimens were examined histologically. Manifold hyperplastic, inflammatory, dysplastic, metaplastic and toxic alterations on the urothel, tumor cells and the lamina propria were found. This wide spectrum is the consequence of various etiological factors. On such treated cancer bladders the therapy-, age- and TuR-conditioned pathomorphological findings are running up with the known panurothelial reactions of a primary tumor in the urinary tract. We have classified the occurrence of multiple single cell necroses within the tumors as obligatory therapeutic effects. The granulomatous and degenerative stromal findings be conditioned by the therapy, if they are localized safely outside the area of previous TuR. The other reactive hyper- and dysplastic reactions be conditioned facultatively by the therapy.
Administration, Intravesical↗
Meiotic nondisjunction in oocytes from aged Djungarian hamsters correlates with an alteration in meiosis rate but not in univalent formation.
The effect of maternal ageing on the meiotic rate, on chiasma and univalent frequency as well as on heteroploidy in secondary oocytes from Djungarian hamsters was examined. The frequency of hyperhaploid oocytes increased from 0.6% in young (8-14 weeks) to 2.8% in middle-aged (26-46 weeks) and reached 3.6% in the oldest females (49-75 weeks). On the basis of malsegregated bivalents per oocyte, nondisjunction occurred most often in the middle-aged group (5.42 X 10(-2) bivalents per oocyte). Hereby, the large meta- and submetacentric A-D chromosomes were preferentially involved. Furthermore, the pattern of nondisjunction was not different from that expected on the basis of chromosome length or induced by colchicine. The large A-D chromosomes did not show any alteration in chiasma or univalent frequency. Terminalized chiasmata were only detected in the E group and univalents increased slightly, but not significantly in the small chromosomes (G group). At higher ages, both chromosome groups were not preferentially involved in nondisjunction. Presegregation slightly increased with age and affected more or less all bivalents, whereas the incidence of diploidy significantly decreased. With respect to the rate of meiosis in oocytes from aged females, the resumption was delayed at metaphase I. Our data suggest that failures in the control of oocyte proliferation are involved in nondisjunction rather than the "production-line." Furthermore, a model is proposed to explain nondisjunction of specific bivalents at certain maternal ages.
Aging↗
Absence of specific luteinizing hormone-releasing hormone (LHRH) receptors in the ovaries of Djungarian hamsters (Phodopus sungorus).
High affinity binding sites for luteinizing hormone-releasing hormone (LHRH) were characterized in Djungarian hamsters. Scatchard analysis was used to demonstrate specific LHRH-binding in hamster and, serving as controls, rat pituitaries (dissociation constant, KD = 0.6 nM, binding capacity, BM = 2.5 +/- 0.7 fmol/mg tissue; KD = 0.6 nM, BM = 6.9 +/- 1.9 fmol/mg tissue, respectively). In contrast to results obtained with rat ovaries (KD = 0.9 nM, BM = 3.0 +/- 0.9 fmol/mg tissue), no specific LHRH-binding was detected in hamster ovaries. Thus, it seems that direct gonadal action of LHRH in the Djungarian hamster is not involved in ovarian regulation.
Animals↗
Follicular development in immature Djungarian hamsters (Phodopus sungorus) and the influence of exogenous gonadotropins.
This study evaluated follicular development and oocyte growth in ovaries of immature Djungarian hamsters from 8 to 28 days of age and examined the influence of exogenous gonadotropins on follicular growth. An age-specific pattern of progressive follicular development was found, beginning with a compact, virtually undifferentiated ovary containing mostly small follicles on Day 8 postpartum and progressing to an ovary with mature preovulatory follicles at the end of the fourth week. Antral follicles not present on Day 12 were first detected on Day 16 postpartum. Follicles were sensitive to gonadotropins (GTH) by Day 12 postpartum, as indicated by the stimulation of follicular maturation by treatment with GTH. Ovulation, however, could be induced only when treatment with GTH was begun with females from Day 14 postpartum onwards. It was concluded that the injected GTH initiated and enhanced follicular growth in immature Djungarian hamsters.
Age Factors↗
[Cancer of the operated stomach].
On the basis of the own documents of patients and the documents of the post-mortem examination the increased risk of carcinoma of the resected stomach is confirmed. Extensive experiments on the rat seem to ascribe the greatest role in the etiology of this special form of gastric cancer to the lesion of the mucous by the bile reflux and the settlement of the operated stomach with nitrate-reducing germs.
Adenocarcinoma↗
Mechanisms of nondisjunction: facts and perspectives.
An ordered segregation requires distinct processes of differentiation within the germ cell for recognition and segregation of homologous chromosomes/chromatids. These include synchronous maturation of the nucleus and cytoplasm, chromosome pairing and assembly at the metaphase plate, and movement within the spindle; all of them may be under direct/indirect regulatory control by the surrounding somatic compartments. Interference, e.g., by hormonal alterations at any of these steps, may alter the normal program of differentiation, thus increasing the risk of chromosomal malsegregation. Hence, many different causative mechanisms may exist which basically, nevertheless, act via (a) nonsegregation, (b) chance segregation, both during meiosis 1 and 2, or (c) presegregation during meiosis 1. In our animal models of the Djungarian hamster and the NMRI/Han mouse strain, we are analyzing the mechanisms of nondisjunction and presegregation during meiosis 1 in oocytes, as well as during aging of the females, by the application of hormones (gonadotrophins and steroids) and specific microtubular inhibitors (colchicine and methylbenzimidazolcarbamate). We suggest that chromosomes play a relatively passive role, although chromosomal properties, e.g., length, chiasma number, NORs, and position within the spindle, may provide an individual risk for each bivalent to be affected by nondisjunction. Failures in the endocrine control of follicular and germ cell maturation are considered to be primary causes for nondisjunction in young and aging oocytes. The understanding of the differentiation processes resulting in the maturation of follicles "at risk" may provide us with the tool to prevent the generation of aneuploidy in man.
Abortion, Spontaneous↗
Correlation between in vitro autoradiographic results and histological WHO grades in human urothelial bladder tumours.
253 biopsy specimens of 106 urinary bladder tumours have been examined by means of in vitro autoradiography and the results compared with the WHO grading. An increase of the average labelling index occurred parallel to increasing WHO grades. However, great inter-tumorous differences in proliferation behaviour existed particularly in papillary G2 and G3 urothelium carcinomas. Possible causes of this heterogeneity are discussed. In vitro autoradiography is a suitable method for the objective characterization of the malignancy potential of urinary bladder tumours.
Adenocarcinoma↗
On the varying degrees of cancerogenicity of modified gastroentero-anastomoses.
In our serial investigations we checked the incidence of carcinomas in different cases of anastomoses and resections on the rat stomach. Moreover we supervised the regenerative behaviour of the gastric mucosa by means of in vivo autoradiography using 3H-thymidine. The experimental animals were divided into 5 treatment groups. We carried out gastroenterostomy on 9 animals (group I), Billroth's second operation on 7 animals (group II), a duodenal ligature, pyloroplasty and gastro-enterostomy with Braun's anastomosis on 10 animals (group III), a pylorectomy as well as Roux's gastro-enterostomy on 10 animals (group IV) and a laparotomy on 11 control animals (group V). The greatest tumour onset rates with 4 adenocarcinomas each were observed in the groups I and III, and with 5 carcinomas in group II 7 months after the operations. The labelling indices obtained autoradiographically are such that glandular hyperplasias with indices of over 13.3 p.c. and carcinomas with values of about 24.7 p.c. enable an unambiguous distinction from the values of the sound glandular gastric mucosa. Entero-gastric reflux is discussed in this paper as the most important cause of carcinogenesis.
Adenocarcinoma↗