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Biomedical subjects

F Zhong

Publications and source records attributed to F Zhong.

26 records · Page 2Linked to original sources
PubMed1995

Methionine-enkephalin augments interleukin-6 production and gene expression.

AIM: To study the effect of methionine-enkephalin (met-enk) on the production and gene expression of interleukin-6 (IL-6) both in vivo and in vitro. METHODS: IL-6 was assayed using its dependent cell line MH60.BSF2 and measured by MTT spectrophotometry. IL-6 gene expression was proceeded by RNA isolation and hybridization with IL-6 cDNA. RESULTS: Met-enk stimulated IL-6 mRNA expression and increased its stability. Met-enk 0.1 and 1 mg.kg-1 ip for 6 d enhanced serum IL-6 levels. CONCLUSION: An up-regulation of IL-6 by met-enk was mediated through an increase in transcriptional activity and stability of IL-6 mRNA.

Animals↗
PubMed1995

[Tyr-D-Ala-Gly-Phe-D-Leu (DADL) solid-phase synthesis and its effects on immune function].

DADL, a delta-receptor selective enkephalin analogue, was prepared with the technique of solid-phase peptide synthesis. It was found to stimulate directly the proliferation of mouse splenic lymphocytes dose-dependently. However, synergistic effect of DADL at different concentrations with ConA and LPS were not found. In addition, TNF-alpha production was increased by ip DADL (1.0 mg.kg-1 x 6 d) in mice and the production of LPS-induced TNF-alpha in mouse peritoneal macrophages was enhanced after DADL was added in vitro. These results suggest that DADL, like enkephalins, exerts some immunoregulatory activities.

Adjuvants, Immunologic↗
PubMed1995

[Tissue culture of bulbus Fritillariae cirrhosae].

On the MS culture medium containing some phytohormones, regenerated Bulbus Fritillariae Cirrhosae can induce without low temperature callus which differentiates into a plant. The total alkaloid content of the culture is two-four times that of the stock and commercial Bulbus Fritillariae Cirrhosae. The free-protein atlas of the culture is consistent with that of the stock.

Culture Media↗
PubMed1994

Evidence that levels of the dimeric cellular transcription factor CP2 play little role in the activation of the HIV-1 long terminal repeat in vivo or following superinfection with herpes simplex virus type 1.

The dimeric transcription factor CP2 binds a sequence element found near the transcription start site of the human immunodeficiency virus (HIV-1) long terminal repeat. Several groups have suggested that cellular factors binding this element might play a role in modulating HIV-1 promoter activity in vivo. For example, induction of latent HIV-1 gene expression in response to superinfection by herpes simplex virus type 1 (HSV-1) or cytomegalovirus is thought to be mediated, in part, by factors binding the CP2 site. In this report we began to examine directly the relationship between CP2 and expression of the HIV-1 promoter. First, we tested what effect HSV-1 infection of T cells had on the cellular levels of CP2. The results showed that HSV-1 infection led to a significant reduction in the level of CP2 DNA binding activity and protein within 20 h. Next, we tested the effect of overexpressing either the wild-type factor or a dominant negative variant of CP2 on HIV-1 promoter activity in vivo. The results showed that CP2 had little effect or slightly repressed HIV-1 promoter activity in vivo. In addition, these expression constructs had little effect on the induction of HIV-1 promoter activity elicited by HSV-1 infection.

Amino Acid Sequence↗