Biomedical subjects

Fangju Yang

Publications and source records attributed to Fangju Yang.

5 recordsLinked to original sources

PubMed2005

[Study on the antipyretic, analgesic effects of qingkailing freeze-dried powder for injection and its antipyretic mechanism].

OBJECTIVE: To investigate the antipyretic, analgesic effects of Qingkailing Freeze-Dried Powder for Injection (QI) and its mechanism of the antipyretic effect. METHODS: The rabbit model of 2, 4-dinitrophenol-induced fever and the rat model of endotoxin-induced fever were established and the body temperatures were observed. Radioimmunoassay was adopted to detect the levels of IL-1beta and cAMP in the hypothalamus of rats. The analgesic effects were tested by hot-plate and writhing reaction method. RESULTS: QI showed marked antipyretic effects on the 2,4-dinitrophenol-induced fever and the endotoxin-induced fever. At the same time, QI remarkably decreased the contents of IL-beta and cAMP in the hypothalamus. QI also remarkably reduced the frequency of acetic acid-induced writhing and delaied the time of licking rear feet. CONCLUSION: QI has antipyretic, analgesic effects. Its mechanism of the antipyretic effects on rats may be inhibiting the production of IL-1beta and accordingly decreasing cAMP in the hypothalamus.

2,4-Dinitrophenol↗

PubMed2004

[Effects of Salviae miltiorrhizae on H2O2-induced apoptosis of rat vascular smooth muscle cells].

OBJECTIVE: To investigate the effects of Salviae miltiorrhizae on H2O2-induced apoptosis in rat vascular smooth muscle cells (VSMC(s)) and its related mechanism. METHODS: The 3-5 passages of VSMC(s) were used, H2O2 was added to the medium of cultured rat aortic VSMC(s) as extraneous reactive oxygen species (ROS). The viability percentage of VSMC(s) was evaluated by MTT colorimetric assay. The percentage of apoptosis was measured by flow cytometry. The expression of Bax/Bcl-2 protein was examined with immunohistochemical technique and positive index (PI) was calculated. RESULTS: 1 mmol/L H2O2 could increase apoptosis rate. The PI of Bax/Bcl-2 protein in H2O2 group was higher (P < 0.01). When VSMC(s) were incubated with H2O2 plus Salviae miltiorrhizae, the apoptosis rate greatly declined, and at the same time, the PI of Bax/Bcl-2 protein reduced remarkably compared with H2O2 group (P < 0.05 or P < 0.01). CONCLUSION: Salviae miltiorrhizae could prevent VSMC(s) from H2O2-induced apoptosis by decreasing the Bax/Bcl-2 protein expression.

Animals↗

PubMed2003

[Effect of shenfu injection on microcirculation].

This study was aimed to assess the effect of Shenfu injection on different circulation state. Using a microcirculation microscope system, we observed mice's auricle micro-artery diameter, density of capillary, blood velocity in different circulation state (i.e. normal state, epinephrine or endotoxin induced microcirculation disturbance state) after administering Shenfu injection into their caudal vein, and we compared the Shenfu group with Shenmai group and Dexamethasone group. The results showed that Shenfu injection causes the auricle microartery diameter to enlarge and the density of capillary and blood velocity to increase in different microcirculation state, and such effect is especially notable on the epinephrine induced microcirculation disturbance group and endotoxin induced microcirculation disturbance group; the effect of Shenfu injection is stronger than that of Shenmai injection and similar to Dexamethasone injection. In addition, Shenfu injection was shown to have remarkable effect on resisting the lowering of limb temperature when the mice are attacked by endotoxin.

Animals↗

PubMed2003

[The mechanism of Radix Salviae Miltiorrhizae Injection on the inhibition of smooth muscle cell proliferation induced by H2O2].

OBJECTIVE: To observe the effect of Radix Salviae Miltiorrhizae Injection (RSMI) on proliferating cell nuclear angiten (PCNA), NF-kappa B and cell cycle phase of vascular smooth muscle cell(VSMC) induced by H2O2 in vitro. METHODS: H2O2 was added to the medium for culturing rat aortic VSMC as extraneous reactive oxygen species (ROS). The protein expression of PCNA and NF-kappa B was examined with immunocytochemical technique in VSMC, and cell cycle phase analysis was used to detect proliferation of VSMC. RESULTS: The result showed that the protein expression of PCNA and NF-kappa B became stonger, the ratio of S phase cells became higher in H2O2 group. RSMI could increase the percentage of the cells in G0/G1 phase, decrease the percentage of the cells in S phase and obviously inhibit the expression of PCNA and NF-kappa B proteins. CONCLUSIONS: The expression of PCNA and NF-kappa B was inhibited and the transition from G0 phase to S phase was blocked, which may be one of the key mechanism of RSMI on the inhibition of VSMC proliferation induced by H2O2.

Animals↗

PubMed2003

[Experimental study on estrogenic function of triphasic contraceptive tablets].

OBJECTIVE: To assess the estrogenic effect and activity of oral triphasic contraceptive tablets and provide the theoretical foundation for the use of homemade Kalirui in the treatment of metrorrhagic, menometrorrhagic, oligomenorrheic, and polymenorrheic dysfunctional uterine bleeding. METHODS: The vaginal smears of mice treated by triphasic contraceptive tablets (Kalirui and Trinordiol) were examined using light microscope. Radiational receptor analysis was made to measure the estrogenic effect of Kalirui and Trinordiol on Compared with the control group, the three dosage groups of homemade (Kalirui) rabbit uterus. RESULTS: triphasic contraceptive tablets and the import tablets (Trinordiol) group all showed obviously accelerate vagina kera-epithelium forming (P < 0.01), especially in the first phase and second phase. Both Kalirui and Trinordiol were found to have higher affinity to estrogenic receptor, and there were no significant differences between them Kalirui and Trinordiol triphasic oral contraceptive and the estrogenic standard sample (P > 0.05). CONCLUSION: tablets have apparently estrogenic effect and activity.

Animals↗