Biomedical subjects

Fehmi Doner

Publications and source records attributed to Fehmi Doner.

9 recordsLinked to original sources

PubMed2005

Nitric oxide level in the nasal and sinus mucosa after exposure to electromagnetic field.

OBJECTIVE: The purpose of this study was to examine the changes in nitric oxide (NO) level in the nasal and paranasal sinus mucosa after exposure radiofrequency electromagnetic fields (EMF). STUDY DESIGN AND SETTING: Thirty male Sprague-Dawley rats were randomly grouped as follows: EMF group (group I; n, 10), EMF group in which melatonin received (group II; n, 10) and the control (sham operated) group (group III; n, 10). Groups I and II were exposed to a 900 MHz. Oral melatonin was given in group II. Control rats (group III) were also placed in the tube as the exposure groups, but without exposure to EMF. At the end of 2 weeks, the rats were sacrificed, and the nasal and paranasal sinus mucosa dissected. NO was measured in nasal and paranasal mucosa. RESULTS: The nasal and paranasal sinus mucosa NO levels of group I were significantly higher than those of the control group (group III) ( P < 0.05). However, there was no statistically significant difference between group II and the control group (group III) regarding NO output ( P > 0.05). CONCLUSION: Exposure to EMF released by mobile phones (900 MHz) increase NO levels in the sinus and nasal mucosa. SIGNIFICANCE: Increased NO levels may act as a defense mechanism and presumably related to tissue damage. In addition, melatonin may have beneficial effect to prevent these changes in the mucosa.

Animals↗

PubMed2004

The role of free oxygen radicals on the development of otitis media with effusion.

OBJECTIVE: The purpose of this study was to determine if free oxygen radicals (FORs) and antioxidant enzyme activities have some role in pathogenesis of otitis media with effusion (OME) in children with adenoid hyperplasia. METHODS: Seventy-four patients were enrolled in three groups of this study. The study group (Group I) included 26 patients who had adenoidectomy with ventilation tube placement due to chronic OME. The control adenoid group (Group II) consisted of 28 age-matched patients who had adenoidectomy without ventilation tube insertion. Twenty children were included in the healthy control group (Group III). Erythrocyte malondialdehyde (MDA) levels, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) enzyme activities were investigated in the venous blood sample. RESULTS: Erythrocyte MDA level and GSH-Px enzyme activity in the blood samples of study group (Group I) were significantly higher than those of Group II and Group III (P < 0.05). SOD enzyme activity in the blood samples of Group I was significantly lower than Group II (P < 0.05), and were significantly higher than Group III (P < 0.05). CAT enzyme activity of Group I was significantly lower than that of Group III (P < 0.05). However, there was no statistically significant difference between Group I and Group II regarding CAT antioxidant enzyme activity (P < 0.05). CONCLUSIONS: The inflammation of the middle ear increases the level of FORs in erythrocyte. FOR level is normally maintained at a steady state by antioxidant enzymes. When the antioxidant defense system is weakened, the increased FORs may contribute to OME formation. We supposed that, antioxidant vitamins C and E, and scavenger enzymes such as CAT, SOD and GSH-Px may be added in the management of OME.

Adenoidectomy↗

PubMed2004

Allergic contact dermatitis prevalence in patients with eczematous external otitis.

Eczematous external otitis, because of its basic allergic nature, tends to be chronic or recurrent. The purpose of our study was to investigate the association between allergic contact dermatitis and chronic eczematous external otitis. Sixty-six patients with eczematous external otitis and 48 healthy volunteers were included in this study. All the patients were tested with an epicutaneous patch test. In the study group, the average recurrent attack rate was 6.1+/-1.5 (between 4-9 times during the last year) in the patch test positive eczematous external otitis patients, and 4.9+/-1.3 (between 4-8 times during the last year) in the patch test negative patients (p = 0.002). The epicutaneous patch test was positive in 19 (28.8%) out of 66 cases with chronic eczematous external otitis, and in 3 (6.3%) out of 48 cases in the control group. The difference between the two groups was statistically significant (p = 0.003). The most common reactions were due to neomycin sulfate and potassium dichromate in this study. These results suggest that, in some cases eczematous external otitis may be considered as a form of delayed type hypersensitivity to allergen stimuli. Patients suffering from eczematous external otitis symptoms should be investigated for allergens.

Adolescent↗

PubMed2003

The biochemical analysis of tympanosclerotic plaques.

OBJECTIVE: We conducted a biochemical analysis of tympanosclerotic (TS) plaques. METHODS: Forty-five samples containing TS plaques were obtained from 45 patients. Qualitative and quantitative biochemical analyses were performed. TS plaques were evaluated macroscopically in respect to color and structure. RESULTS: Phosphate, calcium, ammonium, and cholesterol were detected in 44, 43, 35, and 7 samples, respectively. Mean calcium and phosphate levels and protein levels of samples were 2.5 +/- 2.6 mg/100 mg tissue, 0.16 +/- 0.11 mg, and 3.4 +/- 3.4 mg, respectively. TS plaques were dirty white in 20 samples, brunette in 13, yellowish in 4, white in 7, and brown in 1. The structure of TS plaques was hard in consistency in 23 samples, fibrous in 14 samples, and soft in 8 samples. CONCLUSION: The wider range of values and different mineral contents in our series might be explained by the fact that we had a much larger variety of tissues in various stages of the TS process. The color and the consistency of TS plaques are due to the discrepancy in their content and the amount of mineral, cholesterol, and protein.

Adolescent↗

PubMed2003

New sinus culture applicator (Yariktas applicator).

Identification of the pathogen is crucial for the selection of an appropriate antibiotic in the management of sinusitis. We describe an original instrument that provides contamination-free culture from the sinus in endoscopic sinus surgery.

Humans↗

PubMed2003

Rhinosinusitis among the patients with perennial or seasonal allergic rhinitis.

Although no hypothesis is universally accepted, allergic rhinitis (AR) may play an important role in the occurrence of rhinosinusitis. This study was carried out to investigate the frequency and severity of rhinosinusitis among patients with seasonal or perennial AR. This study included 73 patients with AR. Findings related to rhinosinusitis of these patients were demonstrated by computed tomography (CT), and were scored according to the Lund-Mackay CT scan staging system. Fifty-two of the 73 patients (71.2%) were shown to have findings of rhinosinusitis. Of these 52, twenty-four patients (77.4%) had perennial AR and 28 (66.7%) patients had seasonal AR. The mean total CT staging score of rhinosinusitis was 4.2 +/- 3.2 in patients with perennial AR and 2.6 +/- 1.3 in patients with seasonal AR. Although the frequency of rhinosinusitis among patients with perennial AR was higher than among seasonal ones, this was statistically insignificant (p = 0.32). But CT staging scores of rhinosinusitis among the patients with perennial AR were significantly higher than among the seasonal AR group (p = 0.014). Although there was no significant difference between the frequency of rhinosinusitis of seasonal and perennial AR, the severity of rhinosinusitis was more severe in cases with perennial AR, in this study. Early management of an allergic disease is essential if its long-term consequences are to be prevented or minimized.

Adult↗

PubMedDate not supplied

Asymptomatic food hypersensitivity prevalence in patients with eczematous external otitis.

PURPOSE: The purpose of our study was to investigate the association between food hypersensitivity and eczematous external otitis. MATERIALS AND METHODS: In this cross-sectional study, 27 patients with eczematous external otitis and 16 healthy volunteer were included. The existence of food hypersensitivity was defined by positive epicutaneous prick test and specific immunoglobulin E (IgE) antibodies. RESULTS: Food allergy test was positive in 13 (48.1%) out of 27 cases with eczematous external otitis, and it was positive in 2 (12.5%) out of 16 cases in control group. The difference between 2 groups was statistically significant (P =.02). Specific IgE seropositivity (51.9%) was high in the patients with eczematous external otitis compared with the control group (12.5%) (P =.02). CONCLUSIONS: Food hypersensitivity may be a factor, which should be considered in eczematous external otitis patients. Patients suffering from eczematous external otitis symptoms should be evaluated for food hypersensitivity.

Adolescent↗

PubMedDate not supplied

Intercellular adhesion molecule-1 expression in nasal polyps treated with corticosteroid.

PURPOSE: The aim of this study was to compare intracellular adhesion molecule-1 (ICAM-1) expression in nasal polyp cases who were administered topical corticosteroid and in middle turbinates. MATERIALS AND METHODS: Twenty-four patients with nasal polyps were included in the study group. These patients were treated with 100 microg budesonide in each nostril twice daily for 2 months before the surgery. Twenty-one nonatopic patients with concha bullosa were included in the control group. The specimens were taken from patients undergoing endoscopic surgery. RESULTS: In polyps, significantly higher mean ICAM-1 intensity scores were found by comparison with the control turbinates. CONCLUSIONS: Corticosteroid treatment in patients with nasal polyps does not diminish ICAM-1 to that of turbinate tissue. The initiating events in the formation of nasal polyps still occur in these patients despite treatment with the topical nasal steroid.

Administration, Intranasal↗

PubMedDate not supplied

The effect of topical corticosteroid on basic fibroblast growth factor in nasal polyp tissue.

BACKGROUND: The etiology of nasal polyposis and pathophysiological mechanisms of polyp formation is still poorly understood. Experimental models have suggested that nasal polyp growth requires extracellular matrix formation and is associated with fibroblast proliferation. Intranasal corticosteroids appear to be useful in reducing nasal polypoid lesions and the likelihood of polyp recurrence after surgery. Basic fibroblast growth factor (bFGF) is a potent angiogenesis factor and is mitogenic for a wide range of cell types. We investigated the alteration of bFGF levels in nasal polyp tissue after administration of topical corticosteroid. METHODS: Nasal polyp tissues were obtained from 36 patients with diffuse nasal polyposis before and after topical nasal steroid treatment. As a topical nasal steroid mometasone furoate was given for 4 weeks in a dosage of 200 microg/day. The bFGF levels were measured by competitive enzyme immunoassay method. RESULTS: The mean levels of tissue bFGF, before and after topical nasal steroid treatment, were 1485 +/- 826 ng/mg protein (range, 416-3434 ng/mg) and 1340 +/- 749 ng/mg protein (range, 330-3288 ng/mg), respectively. The levels of bFGF in nasal polyps were significantly lower than those before treatment after administration of topical nasal steroid (p = 0.011). CONCLUSION: Administration of topical nasal steroid decreases bFGF levels of nasal polyp. It may be suggested that one of the effects in diminishing the size of nasal polyps is by decreasing the bFGF.

Administration, Intranasal↗