Biomedical subjects

G A Spengler

Publications and source records attributed to G A Spengler.

At least 19 recordsLinked to original sources

PubMed1983

CSF protein values in infants and children.

Total protein, albumin and gamma-globulin values were determined in CSF of 139 children. They had been referred to the emergency department of the Children's Hospital of the University of Berne during the last year because of suspected meningitis which, however, was not confirmed. In newborns and infants the normal range of protein values was wide, 0.1-0.65 g/l. It fell to minimal values at age 3-4 years, and increased gradually thereafter to adult levels which were reached at age 6-8 years. The observed changes during early childhood are mainly due to variations in the albumine fraction. The results are different from the ones reported by Ammon and Richterich [1] who claimed constantly falling protein levels during childhood up to adult values. Similar results in comparison to ours were obtained by other laboratories using the same method [6,7]. Our study therefore supports the usefulness of Bradford's [2] method for routine CSF work-up.

Adolescent↗

PubMed1982

[Incidence of accidentally discovered paraproteinemias among the patients of a hospital for internal diseases].

Covering a ten-year period (1970-1979), this retrospective study was performed to determine the frequency of unexpected paraproteinemia in hospitalized patients with various internal diseases. The survey was made possible by the fact that serum protein electrophoresis had been routinely performed in all patients upon admission. In 86 cases (0.66% of all admissions) monoclonal gammopathy was an unexpected finding. 5 out of these 86 cases (0.038% of all admissions) had malignant disease (2 patients with Bence Jones myeloma, 3 patients with Waldenström's macroglobulinemia), and 3 more cases had lymphoproliferative syndromes. In all other cases the abnormal serum protein findings corresponded to asymptomatic ("benign") paraproteinemias when first detected. In addition, the following statistical parameters were analyzed: distribution among immunoglobulin classes; sex; and concomitant diseases. A critical evaluation was possible in 67 cases of so-called idiopathic paraproteinemia.

Aged↗

PubMed1980

PHA selection electrophoresis--a screening method for monomeric IgM.

Pentameric IgM is precipitable by PHA whereas monomeric IgM is not. During electrophoresis in PHA-containing agar gel pentameric IgM is selected by precipitation from concomitant monomeric IgM which may be visualized separately as a precipitation line with specific antibody. This antibody-like use of the lectin in PHA selection electrophoresis provides a quick and simple method of screening macroglobulinemic sera and other biological fluids for the presence of naturally occurring monomeric IgM subunits.

Absorption↗

PubMed1978

[Lymphoproliferative diseases with small molecular (monomere) IgM paraproteinemia (proceedings)].

Phytohemagglutinin P, when used like an antiserum in immunoelectrophoresis, precipitates with pentameric IgM but not with monomeric IgM subunits. In a retrospective study this simple screening method was applied to 180 sera containing monoclonal IgM. Five cases were found to be of the monomeric type of IgM paraproteinemia. Four of these patients had lymphoproliferative diseases with lymphocytic infiltration of the bone marrow. The last case was complicated by acute myelomonocytic leukemia.

Bone Marrow↗

PubMed1978

IgM and IgG antibody profiles after immunization of man with a HDCS-vaccine (preliminary communication).

HDCS vaccine was given to 20 healthy, not rabies-exposed people on days 0, 3, 7, 14, 28 and 90. Ten of them received in addition 20 IU/kg of human antirabies immunoglobulin. Vaccine potency in NIH tests was 1.8 (WHO). Most individuals did not show any measurable neutralizing antibody titer on days 3 and 7. On days 14, 28, 90 and 300 the mean of titres was above 1:200; with one exception, no titer was lower than 1:50. The sera of days 14 and 90 were separated on "Sephadex G-200" columns. On day 14 at least 46% of the rabies antibody was found in the IgM fraction, while 15% was recovered from the IgG fraction. On day 90 the proportion recovered from the IgM fraction had decreased to 17%; the IgG antibody had increased to 30% of the titres found in whole serum. Almost no antirabies activity was found in the IgG fractions.

Antibody Formation↗

PubMed1977

[Clinical-immunological tests; current state].

Explore the source record for details and available documents.

Autoimmune Diseases↗

PubMed1976

The origin of serum protein, A, B, and H blood group, and Gm and Inv antigens in house dust.

Materials precipitated from an aqueous extract of house dust by saturation with ammonium sulphate showed immunological reaction with antisera to human serum albumin, human alpha1-acid glycoprotein, human IgG, Gm and Inv antigens and to A, B and H antigens. It is concluded that the albumin and alpha1-acid glycoprotein are of human origin. It seems that the apparent IgG activity is due to cross-reactivity since, from the anticipated specificities of the Fab and Fc fragments of human IgG, the former could not be detected, and since Gm (6) activity was present, unexpectedly, in Swiss house dust. The A, B and H blood group antigenic activities were detected in relative concentration different from those expected if they were solely of human origin. It is concluded that they are from both human and non-human sources.

ABO Blood-Group System↗

PubMed1975