PHA selection electrophoresis--a screening method for monomeric IgM.

G A Spengler; R M Weber

doi:10.1016/0022-1759(80)90118-0

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PHA selection electrophoresis--a screening method for monomeric IgM.

G A Spengler·R M Weber

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Abstract

Pentameric IgM is precipitable by PHA whereas monomeric IgM is not. During electrophoresis in PHA-containing agar gel pentameric IgM is selected by precipitation from concomitant monomeric IgM which may be visualized separately as a precipitation line with specific antibody. This antibody-like use of the lectin in PHA selection electrophoresis provides a quick and simple method of screening macroglobulinemic sera and other biological fluids for the presence of naturally occurring monomeric IgM subunits.

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G A Spengler, R M Weber. 1980. PHA selection electrophoresis--a screening method for monomeric IgM.. https://doi.org/10.1016/0022-1759(80)90118-0

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