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Biomedical subjects

G Acuña

Publications and source records attributed to G Acuña.

12 recordsLinked to original sources

Acute Chagas' disease (Trypanosomiasis americana) in acquired immunodeficiency syndrome: report of two cases.

Two heterosexual men, aged 31 and 40 years, with the acquired immunodeficiency syndrome and presenting with the acute form of Chagas' disease are reported. The first patient, a carrier of hemophilia A, was treated for 20 years with Chilean and Brazilian cryoprecipitates. This patient acquired both diseases through this medium. The second patient, an inhabitant of northern Chile (fourth region), was allegedly bitten by Triatoma infestans and was an intravenous drug addict. The hemophilic patient presented with a neurologic syndrome; a brain biopsy showed a necrotizing encephalitis with an obliterative angiitis and abundant macrophages. The second patient developed intractable congestive heart failure; necropsy showed a dilated myocarditis with rupture of myofibers and an inflammatory infiltrate rich in plasma cells, lymphocytes, and macrophages. Using light and electron microscopy, abundant amastigotes of Trypanosoma cruzi were seen in brain tissue, especially in the cytoplasm of macrophages, as well as in some myocardial fibers. In both cases, determination of anti-T cruzi antibodies (indirect hemagglutination technique) and xenodiagnosis were positive.

Acquired Immunodeficiency Syndrome

[Chagas disease with the acquired immunodeficiency syndrome. Clinical cases].

We report 2 patients with AIDS who developed Chagas infection, one with encephalitis, the other with acute myocarditis. The implications of immune depression for the manifestations and course of Chagas disease are discussed. Chagas disease should be considered in patients with AIDS who live in endemic zones and who develop cerebral or cardiac manifestations.

Acquired Immunodeficiency Syndrome

[Acalculous acute cholecystitis caused by Cryptosporidium in a patient with AIDS].

We report a patient with the acquired immunodeficiency syndrome who developed acute cholecystitis. Removal of the gallbladder revealed infection by Cryptosporidium spp. The clinical course after cholecystectomy was favourable. The pathogenesis and therapy of this complication in patients with AIDS is discussed.

Acquired Immunodeficiency Syndrome

Cloning, sequencing and mutational analysis of the cytochrome c552 gene (cycB) from Bradyrhizobium japonicum strain 110.

We report the cloning and nucleotide sequence analysis of the cytochrome c552 gene (cycB) of Bradyrhizobium japonicum strain 110. The gene was identified with help of an oligonucleotide that was designed on the basis of the amino acid sequence determined for purified cytochrome c552 of B. japonicum strain CC705. The cycB gene product has an N-terminal 23-amino acid signal peptide that is missing in the mature cytochrome c552 protein. A B. japonicum cycB insertion mutant was constructed which had no observable phenotypic defects in denitrification and symbiotic nitrogen fixation. Thus, the function of c552 remains unknown.

Amino Acid Sequence

Cloning, sequencing, and mutational analysis of the Bradyrhizobium japonicum fumC-like gene: evidence for the existence of two different fumarases.

The Bradyrhizobium japonicum fumarase gene (fumC-like) was cloned and sequenced, and a fumC deletion mutant was constructed. This mutant had a Nod+ Fix+ phenotype in symbiosis with the host plant, soybean, and growth in minimal medium with fumarate as sole carbon source was also not affected. The cloned B. japonicum fumC gene fully complemented an Escherichia coli Fum- mutant, strain JH400, for growth in minimal medium with fumarate. The predicted amino acid sequence of the FumC protein showed strong similarity to the E. coli FumC protein, Bacillus subtilis CitG protein, Saccharomyces cerevisiae Fum1 protein, and the mammalian fumarases. The B. japonicum FumC protein accounted for about 40% of the total fumarase activity in aerobically grown cells. The remaining 60% was ascribed to a temperature-labile fumarase. These data suggest that B. japonicum possesses two different fumarase isoenzymes, one of which is encoded by fumC. Besides E. coli, which has three fumarases, B. japonicum is thus the second bacterium for which there is genetic evidence for the existence of more than one fumarase.

Amino Acid Sequence

[Cryptococcosis in acquired immunodeficiency syndrome].

The diagnosis of cryptococcal infection was established in 3 patients with AIDS. Infection was meningo-encephalic in all and presented a violent outset. The chemical characteristics of the CSF were normal. Detection of cryptococcal antigen, presence of encapsulated yeast forms revealed by india-ink staining and positive cultures allowed a definitive diagnosis. Response to therapy with amphotericin B alone or in combination with 5-fluorocytosine was poor. A maintenance therapy with fluconazole is recommended.

Acquired Immunodeficiency Syndrome

Interpretation module for screening normal ECG.

This work concerns the development of a module for automatic recognition of normal ECGs, i.e. those whose waves morphology do not suggest physiopathological or structural heart alterations. Such a unit constitutes the central part of an administrative system under development which will aid in the management of ECG testing in the primary care delivery level in Chile. This system greatly contributes to the optimal allocation of resources in order to increase the test delivery coverage and to reduce the social and private costs involved. A main feature of the system is that it operates through the screening of normal ECG. This process is performed in a computerized unit whose core is the interpretation module. The design of such a module uses concepts and methods previously developed for the general problem of medical diagnosis, based on fuzzy set theory. The interpretation module parameters were adjusted considering hypothetical and real data covering a wide variety of normal and pathological cases. Then its performance was tested using more than one hundred patients' records chosen at random. Results of this test are given and a discussion, including a comparison with similar commercial equipment, is provided.

Diagnosis, Computer-Assisted

[The value of cerebral biopsy in patients with AIDS and extensive cerebral lesions].

We performed cerebral biopsy in 4 patients with AIDS and evidence of expanding intracerebral lesions. Three patients had cerebral toxoplasmosis and the fourth had Chagas encephalitis. No patient developed complications. We feel that cerebral biopsy is warranted and safe in patients with AIDS and intracerebral lesions of uncertain origin.

AIDS Dementia Complex