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G Dayian

Publications and source records attributed to G Dayian.

4 recordsLinked to original sources
PubMed1986

Improved procedure for platelet freezing.

Platelets frozen in glycerol-glucose frequently aggregated on thawing or reconstitution. This has been eliminated by using a nonplasma diluent (pH 6.5) and a new freezing bag. The results were improved platelet 14C-serotonin uptake and readily demonstrable aggregation to ADP, epinephrine, collagen and thrombin. 14C-serotonin uptake was at maximum when platelets were frozen at thrombocrits of up to 15%. Hemostatic effectiveness was demonstrated in a severely thrombocytopenic patient with acute myelogenous leukemia in relapse, who was supported entirely with two autologous frozen platelet transfusions for the 13 days before bone marrow activity resumed.

Biological Transport↗
PubMedDate not supplied

A simplified method for freezing human blood platelets in glycerol-glucose using a statically controlled cooling rate device.

An inexpensive, statically controlled cooling rate device consisting of a cassette of aluminum plates and cardboard insulation in a plastic bag was used to freeze platelets in liquid nitrogen with glycerol-glucose as cryoprotectant. Depending on the thermocouple location, the cooling rate in the freezing bag varied between 22.1 and 38.6 C per minute, averaging 33.6 +/- 1.1 C per minute at the center. The post-thaw recovery of platelets frozen with this device and reconstituted in plasma averaged 88.6 +/- 11.7 per cent, compared to 86.1 +/- 9.9 per cent for nonfrozen, but otherwise identically processed platelets. 14C Serotonin uptake after 0.5-hour incubation was 95.9 +/- 1.9 per cent for fresh platelets in platelet-rich plasma, 92.7 +/- 4.4 per cent for nonfrozen processed platelets, and 81.4 +/- 11.8 per cent for frozen platelets, increasing to 85.9 +/- 7.7 per cent after one-hour incubation.

Blood Platelets↗