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PubMed · 3467516

Improved procedure for platelet freezing.

G Dayian·H L Harris·G D Vlahides·J H Pert
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Abstract

Platelets frozen in glycerol-glucose frequently aggregated on thawing or reconstitution. This has been eliminated by using a nonplasma diluent (pH 6.5) and a new freezing bag. The results were improved platelet 14C-serotonin uptake and readily demonstrable aggregation to ADP, epinephrine, collagen and thrombin. 14C-serotonin uptake was at maximum when platelets were frozen at thrombocrits of up to 15%. Hemostatic effectiveness was demonstrated in a severely thrombocytopenic patient with acute myelogenous leukemia in relapse, who was supported entirely with two autologous frozen platelet transfusions for the 13 days before bone marrow activity resumed.

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Biological TransportBlood PlateletsBlood PreservationFreezingHumansLeukemia, Myeloid, AcutePlatelet AggregationPlatelet TransfusionSerotoninTransplantation, AutologousJournal Article

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BibTeXRIS

G Dayian, H L Harris, G D Vlahides, J H Pert. 1986. Improved procedure for platelet freezing.. https://doi.org/10.1111/j.1423-0410.1986.tb01971.x

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