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G Ditscherlein

Publications and source records attributed to G Ditscherlein.

At least 37 records · Page 2Linked to original sources
PubMed1986

[Antiglomerular basement membrane antibody-mediated nephritis: incidence and morphological appearance in biopsy materials].

A glomerulonephritis (GN) of the basement membrane antibody-mediated type was diagnosed in 51 patients (44 men, 7 women) on the basis of immunohistological findings in renal biopsy material in a period of 15 years. This represented about 5% of the cases in which immunohistologic studies gave evidence of glomerular disease. Young men, about 20 years old, were especially affected: 35 patients were men between ages 16 and 25 (69%). In about half the cases there were also pulmonary manifestations (Goodpasture's syndrome). In 63% of the cases there was extracapillary GN with compression and/or marked destruction of the glomerular tuft. Anti-glomerular basement membrane antibody-mediated nephritis was the most frequent cause of a rapidly progressive extracapillary GN in our sample. The broad use of renal biopsy in Goodpasture's syndrome has demonstrated that the morphologic spectrum of renal manifestations is much wider than originally believed. It ranges from normal or minimal findings and mild mesangioproliferative or focal extracapillary GN to severe diffuse extracapillary GN. The rapidly progressive form of extracapillary GN was not significantly more frequent in Goodpasture's syndrome than in the cases of GN without lung findings (62%). Immunohistologic findings apparently can persist unchanged for months and therefore microscopic examinations do not allow reliable conclusions concerning the natural history of immunologic activity in this process to be drawn.

Adolescent↗
PubMed1986

[Pathomorphological differential diagnosis of glomerular lesions in diabetics].

In renal biopsy specimens taken from 165 diabetics 3 cases with amyloidosis and 25 with glomerulonephritis (GN) were found. Among these were 13 cases with membranous and 10 with mesangial proliferative GN. This remarkably high rate may be partly due to selection, but, it can also be explained by the techniques of investigation because most of the GN cases were diagnosed by semithin sections and/or immune histology, respectively. In paraffin sections these alterations may be misinterpreted as diffuse glomerulosclerosis (GS), borderline lesions or normal findings. This is understandable because in mesangial proliferative GN, as well as in diffuse GS, the mesangium is broadened; similarly the spike formation or basement membrane doubling of membranous GN may simulate a thickening of the basement membrane. In addition, the differential diagnosis between GS and other diseases/lesions is discussed. We conclude that the examination of specimens by adequate methods will reveal "nondiabetic" glomerular lesions in diabetics more frequently than previously reported in the literature.

Adult↗
PubMed1985

[Bacteriologic, histologic and functional studies of the kidney in 5 days' therapy of experimental E. coli pyelonephritis with gentamycin. Comparison with 9 days' therapy].

Short-term antibiotic treatment is recommended in infections of the lower urinary tract but its effectiveness is questioned in the upper urinary tract infections. We compared a 5 and a 9 day treatment of experimental E. coli 022 pyelonephritis after unilateral nephrectomy in 127 mal Wistar rats. We used 9 mg gentamycin per kg b.w. twice daily. I131 hippurat excretion was not decreased during the 5 day treatment but was only temporarily diminished during the 9 day treatment. Histologically the severe acute pyelonephritis was decreased after the 9 day treatment but not after 5 days of treatment. Bacteriologically almost all the kidneys were sterile after the 9 day treatment but the majority of the kidneys showed the injected strain of E. coli after the 5 day treatment. The results indicated that the shortened treatment was much less effective in our acute experimental pyelonephritis.

Animals↗
PubMed1985

[Kidney function and bacteriological and histological research in the therapy of experimental E. coli pyelonephritis using trimethoprim-sulfamethoxazole (TMP-SMO)].

40 uninephrectomized male Wistar rats with an experimental E.-coli-022-pyelonephritis (PN) were treated twice daily for 9 days with 30 mg trimethoprim and 150 mg sulfamethoxazole (TMP-SMO) i.p. Bacteriologically most of the kidneys became sterile. Histologically a significant reduction of the frequency of severe PN was found in the treated group. The biologic half-life of 131I-hippuran indicated a decrease of excretory function which was reversible. Urine osmolality and osmotic clearance were increased after oral water loading in 10 untreated control animals with PN but not in the treated group. The 9 day treatment had a favourable effect bacteriologically, histologically and also on renal function.

Animals↗
PubMed1983

[Membranous glomerulonephritis in 10 diabetics].

Membranous glomerulonephritis (MGN) seems to be not infrequent in diabetics. We found it in renal biopsy specimens of 10 patients (from a total of 111 investigated by semithin technique). In all of those specimens which were also examined by immunofluorescence technique the diagnosis was confirmed (6 times out of 44 diabetics). All cases with MGN showed proteinuria, most of them a nephrotic syndrome. In 3 patients we observed a combination of MGN with diabetic glomerulosclerosis (GS). Conventional paraffin sections are inadequate for the diagnosis of MGN and the certain distinction from GS.

Adult↗
PubMed1982

Experimental studies on new liquid embolization mixtures (histoacryl-lipiodol, histoacryl-panthopaque).

In vitro measurements have proved that the polymerization time of n-butylcyanoacrylate (Histoacryl) can be delayed markedly and controlled by adding an oily radiopaque material. Our animal experiments have shown that a mixture of Histoacryl and Lipiodol ultrafluid at a 1:3 ratio provided an optimal embolization material with good flow properties, polymerization time of approximately 7 sec and excellent contrast definition. This mixture may be handled easily, with a minimum of risk involved when used with a 40% glucose solution.

Animals↗
PubMed1980

[Morphological classification of glomerulonephritis (continuation)].

As to glomerulonephritis the morphological classification principle as the most differentiated one stood the test. Nevertheless, up to the present time an unclear situation developed which shall be overcome by a WHO-proposal. In this classification the histologically descriptive findings are the basis, in which immunohistologic and electron-microscopic knowledge is included. But the disease of the patient can be estimated comprehensively only then, when also anamnestic, clinical, paraclinical and etiological data are evaluated which can be given only be the clinician. However, the WHO-classification, too, is not "perfect", but according to our own experiences has vastly proved and gives internationally the possibility of a homogeneous judgment.

Glomerulonephritis↗
PubMed1980

[IgA nephropathy in diabetes mellitus (author's transl)].

Report on a diabetic patient with IgA nephropathy whose kidneys were examined by biopsy four times during a period of four years. Morphologically the typical findings were observed: Outstanding deposits of IgA, hypercellularity and increase of matrix within the mesangium. The findings persisted during the period of observation. The differential diagnosis between IgA nephropathy and a slight diffuse glomerulosclerosis in diabetes mellitus is discussed. IgA nephropathy is diagnosed with certainty only by the aid of immunohistology.

Adult↗
PubMed1980

[The significance of electron microscopy for the assessment of membranous glomerulonephritis (author's transl)].

In 35 biopsy specimens taken from 21 patients with membranous glomerulonephritis (mGN) the ultrastructure of the glomeruli was investigated with special regards to the degradation of the immune complexes. Electron microscopy allows the most comprehensive assessment of the manifold basement membrane changes. This is of high value for the practical diagnosis.

Antigen-Antibody Complex↗
PubMed1980

[immunohistologic findings of kidney biopsies in Wilson's disease].

12 patients with Wilson's disease were examined by means of renal biopsy. In 9 of these patients a slight (4 patients) or a strong proteinuria (5 patients) appeared during the therapy with D-penicillamine. A biopsy was performed in 3 patients before the beginning of the therapy with D-penicillamine. The patients with severe proteinuria showed the largest immunohistological changes (partly distinct immune complex nephritis with epimembranous IgG- and complement-(C3-) depositions -- electronoptically membranous glomerulonephritis. In rebiopsy after a stoppage of the medicament of 1 year only a slight tendency to improvement was to be recognized.

Biopsy↗
PubMed1980

[Morphological problems in diagnostic kidney biopsies].

It was tried to describe the most important problems in the routine bioptic diagnostics of the kidneys which may lead to a restriction of the evidence. The difficulties may be traced back to the examination material (quality and quantity), they concern the limited possibilities of the technical treatment in examination material of out-of-town institutions, and they can be established in the findings themselves. In repeated biopsies, apart from this, the choice of the right time for the repeated diagnostic intervention play a role. The difficulties may be considerably reduced, when the taking of tissue and its pathohistologic judgment are performed by experienced physicians, the adequate morphological additional methods may be applied and there is a close cooperation between clinicians and pathohistologists. Under these conditions the bioptic diagnostics of kidneys is of high effectivity.

Biopsy, Needle↗
PubMed1977

[Goodpasture's syndrome and nephrocalcinosis].

In comparison with literary data is reported on 3 young men with a Goodpasture syndrome. Immunohistologically in all cases a glomerulonephritis of the type of basal membrane antibodies was proved, in one patient also still 14 months after having reached the terminal stage of the renal insufficeincy. In every case the disease led to the dialytic treatment without the necessity of nephrectomies for commanding haemmorrhages of the lungs. The longest survival time was 3 years. -- In 1 patient within metastatic calcifications a pronounced nephrocalcinosis developed. Massive depositions of calcium were found in the basal membranes of the glomeruli and tubuli. It is discussed on the genesis of nephrocalcinosis, in which case a secondary hyperparathyrodism, an elevation of the calcium-phosphate-product, an alkalotic metabolic position as well as the dystrophic renal parenchyma of the rapidly progressing glomerulonephritis of Goodpasture's syndrome are taken into consideration.

Adolescent↗
PubMed1976

[Histological diagnosis of glomerulonephritis].

The morphological diagnosis of glomerulonephritis is a descriptive one. On the basis of the classification after Kettler and co-workers several forms are discussed in short and certain interrelations of these forms are indicated. Data concerning floridity are mostly expected, sometimes, however, they are possible only with reservation. Only with the help of the usual paraffin histology in a part of the biopsy cylinders no clear diagnoses can be made. Above all often the exclusion of minimal glomerular changes is not possible. Newer morphological investigation methods are an essential diag-microscopy; the question of the floridity of nostic enrichment, i.e. the semi-microsection technique, the immunohistology and the electron glomerulonephritis is best to be answered immunohistologically.

Biopsy↗