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G Freundl

Publications and source records attributed to G Freundl.

59 records · Page 4Linked to original sources
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Acrosome reaction of spermatozoa with different morphology.

The acrosome reaction of human spermatozoa with different morphological disturbances was evaluated after application of the triple staining method. After triple staining, spermatozoa which display only a small or an undetectable acrosomal region in Papanicolaou-stained semen smears, turn out to be unable to perform an acrosome reaction, whereas merely post-acrosomally hyper-elongated spermatozoa show a normal ability for acrosome reaction.

Acrosome↗
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[Disintegration of human spermatozoa in flow cytometry of semen].

In some cases, the pretreatment of human spermatozoa produced desintegration of various degrees, while in others a reduced staining was observed. For the evaluation of these phenomena the following techniques were applied: papain/DTE solution at pH 5.5 and 6.4, pepsin solution and a staining procedure without any preceeding decondensation of the spermatozoa nuclei. Pathological spermatozoa from patients with severe disorders of spermatogenesis showed an extended resistance to the pretreatment procedures, the spermatozoa being stained after papain/DTE pretreatment at pH 5.5 only. Contrary to this finding, normal spermatozoa and spermatozoa with slight morphological aberrations desintegrated when papain/DTE solutions were used. In this group the abortion rate was high.

Cytological Techniques↗
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[Pepsin pretreatment of human semen as a standard procedure for flow cytometry].

The 30 minutes pepsin incubation of human semen has proved to be a standard pretreatment procedure for impuls cytophotometry (ICP). This method, in comparison to papain treatment, shows a better preservation of the material and avoids the phenomenon of "disintegration" and reduced staining property. The CV of 1C peak is small enough. Additionally, the immature spermatozoa, earlier dissociated late elongated spermatids, diploid spermatozoa, dual forms and diploid semen cells (spermatogenetic cells, leukocytes, epithelial cells are demonstrated in small peaks, a fact which can help explain the disturbances in pathological cases.

Diploidy↗