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Biomedical subjects

G He

Publications and source records attributed to G He.

At least 127 records · Page 7Linked to original sources
PubMed1993

Radioimmunoimaging of experimental arterial and venous thrombi in dogs with 99Tcm-labelled monoclonal anti-activated platelet antibody SZ-51.

In canine experimental models of femoral arterial and venous thrombosis, the feasibility of thrombus imaging with 99Tcm-labelled monoclonal antibody SZ-51 to an alpha-granule membrane protein (GMP-140) on the surface of activated platelets was investigated. Both the arterial and venous thrombi were clearly discernible at 2 to 8 h after injection of 99Tcm-SZ-51. The suitable imaging time was 4 to 6 h. These results were confirmed by ex vivo images of thrombosed arteries and veins followed by killing the dogs at 8 h postinjection of 99Tcm-SZ-51. Quantitative imaging analysis showed that the ratios between the thrombus and the opposite vessel were increased strikingly over time. The ratios of thrombus to blood or surrounding muscle were 57.09 +/- 12.24 and 177.46 +/- 9.00 for arterial thrombi and 6.43 +/- 4.70 and 83.22 +/- 68.98 for venous thrombi at the time of death. Radiolabelled antibody was cleared from the blood with T1/2 alpha of 16.25 +/- 13.39 min and T1/2 beta of 7.28 +/- 1.15 h. These results show that it is feasible to detect noninvasively thrombus formation in vivo using 99Tcm-labelled monoclonal antibody SZ-51.

Animals↗
PubMed1993

The effect of donor-recipient strain combination on rejection and graft-versus-host disease after small bowel/liver transplantation in the rat.

The initial clinical experience with simultaneous small bowel/liver transplantation (SBL) suggests that liver grafting may protect the small bowel from rejection. A pilot study of SBL in DA (RT1a) rats with Lewis (RT1l) allografts in our laboratory provided experimental support for this concept. However, the clinical applicability of the data was questioned because the transplants were performed in a low-immune-responder rat strain combination. This study examined the outcome of SBL in several rat strain combinations. Isolated small bowel transplants (SB) and SBL were performed in three groups: DA-->PVG (low immune responder), BN-->LEW (intermediate immune responder) and ACI-->LEW (high immune responder). Lewis-->Lewis isografts were used as controls. All of the rats with SB rejected their allografts, whereas all of the rats with simultaneous liver grafts had minimal or no signs of intestinal rejection. The outcome of SBL was profoundly affected by the donor-recipient strain combination. The low immune responders developed severe graft-versus-host disease. The intermediate immune responders developed mild-to-moderate GVHD and moderate liver rejection. The high immune responders developed severe liver rejection. In this study, the outcome of small bowel transplantation depended upon the strain combination used and whether or not a simultaneous liver graft was transplanted. The immune interactions that occur after multi-visceral transplantation are complex and cannot be easily predicted.

Animals↗
PubMed1992

Overexpression of the c-erbB-2/neu-encoded p185 protein in primary lung cancer.

The c-erbB-2/neu gene encodes a transmembrane protein of 185 kDa (p185) with tyrosine kinase activity and extensive sequence homology to epidermal growth factor receptor. Amplification and overexpression of the c-erbB-2/neu gene has been shown in certain human tumors and is postulated to be important in human carcinogenesis. High levels of expression of the c-erbB-2/neu gene have been reported in non-small-cell lung cancer (NSCLC) cell lines and primary tumors from the United States. Since geographical and cultural factors may contribute to the development of certain types of cancer, we examined p185 examined p185 expression in 120 tumors from Chinese patients with lung cancers of different cell types and used immunohistochemical staining to determine the extent and general significance of p185 expression in human primary lung cancer. Our results demonstrate that 58.8% of the NSCLCs expressed p185 and that expression of p185 was observed only in NSCLC and not in small-cell lung cancers. Thirty-three of 41 adenocarcinomas and 24 of 55 squamous cell carcinomas among the NSCLCs examined were found to express p185 at levels different from those of normal lung. For the squamous cell carcinomas, p185 expression was correlated with lymph node metastasis (P less than 0.01), but for the adenocarcinomas, it was not (P greater than 0.05). In addition, expression of p185 in NSCLC was significantly more frequent in patients in advanced clinical stages. Our findings indicate that p185 expression is a frequent event and a general phenomenon in NSCLC and is correlated with poor clinical prognostic indicators, suggesting that expression of p185 may be of potential prognostic importance in NSCLC.

Adenocarcinoma↗
PubMed1992

Surgical technique for combined liver/intestine transplantation in rats.

Simultaneous liver transplantation may reduce the risk of intestinal transplant rejection. We have recently developed two new models of combined liver/intestine transplantation (LIT) in the rat to study this phenomenon. Herein, we report our experience with LIT using a single donor (SD) or multiple donors (MD). Large volumes of fluid were required to prevent a drop in the mean arterial pressure during the anhepatic phase in the SD recipients. Many of the SD recipients died of intraoperative hypovolemic shock (57%). The MD recipients had a shorter anhepatic time (12 +/- 1 minutes vs. 17 +/- 2 minutes; P less than 0.01) and a shorter warm intestinal ischemia time (15 +/- 1 minutes vs. 32 +/- 2 minutes; P less than 0.01). Operative mortality rates were much lower in the MD recipients (10% vs. 68%; P less than 0.01). The long-term survival rate using MD was 71% at 1 month. Graft function was normal in the long-term survivors. LIT with MD provides a good model to study the immunological effects of multivisceral grafting.

Animals↗
PubMed1992

[Influences of naomaitong upon cerebral blood flow in dogs].

This experiment has proven that the drug naomaitong can synchronously increase the blood flow of both dog's internal carotid artery and vertebral artery and also can antagonize the decrease of blood flow of both arteries caused by pituitrin. The increase of blood flow of both internal carotid artery and vertebral artery due to administration of naomaitong (1.2 g/kg) through the dog's duodenum is approximately equal to the increase of blood flow of both arteries resulting from the intramuscular injection of papaverine (1 mg/kg). Besides, the dosage of naomaitong is positively correlated to the effect and action of the drug.

Animals↗
PubMed1992

[Isolation and identification of a strain of Pseudomonas SP. producing insoluble glucan hydrolase].

A strain, CIB871, isolated from the natural world can hydrolyze insoluble glucan(IG) produced by S. mutans. This organism has been identified to be a strain of Pseudomonas SP. It grows well at 30 degrees C. The optimum medium for producing insoluble glucan hydrolase (IGase) is composed of 0.3% peptone, 0.03% IG and M1 salt solution (NH4)2 SO4 1mg/ml, MgSO4 50 micrograms/ml, FeCl2 50 micrograms/ml, K2HPO4 0.5mg/ml). The IGase production reached maximum when cultured for 65-70 hours.

Glucans↗
PubMed1991

Simultaneous pancreaticoduodenal and renal transplantation in one diabetic patient with end-stage renal disease.

The diabetic patient with renal failure underwent simultaneous pancreaticoduodenal and renal transplantation. Duodeno-cystostomy was performed for the drainage of exocrine secretion. Immediately after grafting, the pancreatic and the renal graft regained normal function, and there was no operative complication whatever. The patient had functioning pancreatic graft and became insulin-independent for more than 3 months, although two transplanted kidneys were rejected. Immunosuppression consisted of cyclosporine A, azathioprine and prednisone. Our results suggest that hemorrheological parameters may serve as early indicators of acute rejection and be used to estimate the efficacy of anti-rejection and anti-coagulation therapy.

Acute Kidney Injury↗
PubMed1990

[On the transmission role of residual microfilaremia cases in the area with filariasis virtually eradicated].

The transmission role of the residual microfilaremia cases was studied in Pingle Village, Liangqing Township, Yongning County where bancroftian filariasis had been virtually eradicated during 1984-1988. The results indicated that after the microfilarial rate of the human population met the criterion of elimination of filariasis, namely below 1% of the total population in all the administrative villages, and the average density of microfilaria being around 5 per 60 microliters peripheral blood, subsequent control measures might be no longer instituted while the microfilaria rate and its density could continue to decline. Some residual microfilaremiae cases having higher microfilarial density turned negative gradually, so did the natural infection rate of Culex quinquefasciatus with filaria larvae and its density year by year. No filaria larvae were found in vector mosquitoes in 1987-1988. The authors deemed that the filariasis transmission in this area has been interrupted.

Animals↗