PubMed Health⌕ Search

Biomedical subjects

G Inoue

Publications and source records attributed to G Inoue.

At least 127 records · Page 7Linked to original sources
PubMed1988

The effect of amino acid infusion on partial pressure of oxygen in pelvic urine of the rat.

Renal function decreases with age, but the precise mechanism of the decrease is unknown. Recently, Brenner et al. have proposed the hypothesis that high protein intake may injury renal function in various clinical settings including the aging kidney. Since connective tissue in the renal papilla changes with age, high protein intake may have some deleterious effect on the renal papilla. In this study, the effect of amino acid infusion on partial pressure of oxygen of pelvic urine was examined in the rat. After amino acid infusion, glomerular filtration rate significantly increased and partial pressure of oxygen of pelvic urine decreased, while partial pressure of oxygen of arterial blood remained unchanged. The findings imply that high protein intake may affect the renal papilla both acutely and chronically through renal medullary hypoxia.

Amino Acids↗
PubMed1988

Locked metacarpo-phalangeal joint of the thumb.

16 patients developed locking of the M.P. joint of the thumb as the result of a hyperextension injury. Four were successfully treated by manipulation under an intra-articular injection of a local anaesthetic, while the remaining twelve required open reduction. All were examined 4 to 48 months after treatment (average 12 months). The torn palmar plate and the accessory collateral ligament, which acted as a constrictive band, were trapped on the prominence of the radial condyle of the metacarpal. Four of the twelve surgically-treated patients had a deformity which we believe was congential: a transverse groove over the radial condyle of the metacarpal. No patients had a recurrence of the locked joint.

Adolescent↗
PubMed1988

Revascularization of digits after prolonged warm ischemia.

Four cases of digit revascularization after prolonged warm ischemia are presented. Amputation injuries occurred in the summer, with the average temperature above 23 degrees C. The average warm ischemia time was 24 hr (range: 20 to 30 hr) from injury to the time of establishment of arterial inflow. Complete survival was achieved in three cases and partial survival in one case. Previously, eight to ten hours were believed an acceptable limit for warm ischemia; however, this study indicates that successful revascularization is still possible, even after a much longer period of time.

Adult↗
PubMed1988

Arterialized venous flap coverage for skin defects of the hand or foot.

This is a report on 15 patients whose skin defects of the hand or foot were covered using an arterialized venous flap, with only arterial blood flowing through the vein of the flap. Success appeared to be influenced by the donor site and size of the flap. When a small-size flap from the forearm was used, the success rate was almost 100 percent; however, there was a 50 percent failure rate when a large flap from the leg was used. This technique provides not only flap coverage for exposed bone and tendon, but also provides one-stage procedures for digits in need of revascularization and skin coverage.

Adult↗
PubMed1988

The effects of dietary amino acid levels upon lysine and methionine requirements for growing rats.

The amino acid requirement has been investigated by many laboratories since 1931. Authors considered that the requirement was influenced by the protein level of the basal diet and also by the experimental method. In light of those earlier studies, the present study investigated the effect on lysine and methionine requirements by modifying total nitrogen level or essential amino acid level. The result of our study confirmed that when the amino acid requirement for maximum growth was expressed as the dietary percentage, amino acid to total amino acid ratio, and amino acid to total nitrogen ratio, the requirement had changed according to not only essential amino acids but also to nonessential amino acids. Furthermore, we investigated the relationship between amino acid intake and body protein gain, and found that 6.2 mg lysine and 9.0 mg methionine were required for the maintenance of a rat weighing 80 g and 71.6 mg of lysine and 48.8 mg of methionine were required for one gram of body protein gain. The lysine and methionine intakes required for one gram of protein gain were equivalent to 100% and 124% of amino acid contents of body protein, respectively.

Amino Acids↗
PubMed1988

Optimal protein intake estimated by the resistance to streptococcal infection and the nutritional indices in mice.

Effects of dietary protein levels on the resistance against a bacterial infection and on the nutritional status were studied in mice to obtain basic data for the estimation of an optimal protein intake. Female DDY strain weanling mice were fed 5, 7, 10, 20, 30, or 40% casein diet. At 2 or 4 weeks on the diets, the mice were injected intraperitoneally with 5 x 10(3) or 5 x 10(4) group B streptococci/g body weight and their survival rates were observed for the following 10 days. Nutritional indices and cell numbers of thymus and spleen were also measured. The survival rate was higher in the order of 7, 10, 20, 30, 5, 40% casein diet group. Significant differences were observed between the 7% group and the 30, 5, or 40% group, and between the 10% group and the 5 or 40% group. The nutritional indices and cell numbers of the thymus and spleen were similar among the 20, 30, and 40% casein diet groups and decreased in the order of 10, 7, 5% casein diet groups. From the results, protein levels were categorized into 4 groups: severe protein deficiency with low resistance (5% casein diet), moderate protein deficiency with high resistance (7 and 10% casein diets), normal protein intake with normal resistance (20% casein diet), and high protein intake with low resistance (30 and 40% casein diets). This grouping suggests that when the relation between immunocompetence and nutritional status is considered, both high and low protein intakes are undesirable and the optimal level of dietary protein will be limited to a narrow range.

Animals↗
PubMed1988

Sipple's syndrome with liver tumors examined by iodine-131 MIBG and technetium-99m(V)-DMSA.

This case report describes the localization and categorization of tumors using 99mTc(V)-dimercaptosuccinic acid and [131I]metaiodobenzylguanidine scans in a very uncommon case of medullary thyroid carcinoma associated with pheochromocytoma (Sipple's syndrome) and hepatocellular carcinoma. Technetium-99m(V)-dimercaptosuccinic acid showed accumulation only in medullary thyroid carcinoma, but [131I]metaiodobenzylguanidine scans were positive in both medullary thyroid carcinoma and pheochromocytoma. In advanced Sipple's syndrome, combined use of [99mTc(V)]dimercaptosuccinic acid and [131I]metaiodobenzylguanidine may be useful for the categorization of tumor mass lesions and planning appropriate therapy.

3-Iodobenzylguanidine↗
PubMed1987

Nonoperative treatment of camptodactyly.

Twenty-four patients with camptodactyly of their small fingers were treated with a dynamic splint. Most of the patients could extend the proximal interphalangeal (PIP) joints within a few months by using the dynamic splint 24 hours a day. After almost full extension of the proximal interphalangeal joint was achieved, splinting for 8 hours a day produced good final results. However, after the splint was removed, contracture tended to recur. Although further studies are needed to determine the optimum termination of treatment, in our opinion it should be when the growth plate is closed, and longitudinal growth of the finger no longer occurs.

Adolescent↗
PubMed1985

Comparative effects of protein, protein hydrolysate and amino acid diets on nitrogen metabolism of normal, protein-deficient, gastrectomized or hepatectomized rats.

The effects of diets containing protein, protein hydrolysate or an amino acid mixture on nitrogen metabolism of normal and severely protein-deficient rats and on rats after gastrectomy and hepatectomy were compared. In experiment 1, rats weighing about 80 g were fed one of three experimental diets containing casein, protein hydrolysate or an amino acid mixture for 28 d. In experiment 2, rats weighing about 270 g were fed a protein-free diet for 40 d, and then one of the above three experimental diets for 14 d. In experiment 3, rats weighing about 220 g were totally gastrectomized and fed one of the three experimental diets for 21 d. In experiment 4, rats weighing about 210 g had about 70% of the liver resected, and then were fed one of the three experimental diets for 15 d. In all four experiments, daily food intake, daily body weight gain, nitrogen balance, urinary nitrogen compounds, body composition and hematologic values were measured. In normal, protein-deficient and hepatectomized rats, most results were similar for the groups fed protein, protein hydrolysate or amino acid diets, except that urinary ammonia was higher in the group fed the amino acid diet than in the other two groups. For about 10 d after total gastrectomy, food intake and growth of the animals fed the protein diet were lower than in the other two groups.

Amino Acids↗
PubMed1983

Contributions of individual body tissues to nitrogen excretion in adult rats fed protein-deficient diets.

An attempt was made to estimate the contributions of various tissues to nitrogen (N) excretion in rats fed protein-free diet or 2 and 10% lactalbumin diets for 14 to 31 days. The contributions were calculated as differences between the amounts of dietary N utilized for tissue protein and tissue growth. The amount of dietary N utilized for tissue protein was calculated from the recovery of 15N of orally administered [15N]leucine and N intake. The following results were obtained. 1) With a 10% protein diet, the contribution of tissues to N excretion was affected more by the turnover rates of individual tissues than by the size of the tissues, but with a protein-free diet the contribution was greatly affected by the size of the tissues. 2) With 2% lactalbumin diet, the contributions were not intermediate between those with protein-free diet and 10% lactalbumin diet: urinary N excretion was similar to that with protein-free diet in spite of the difference in N intake, probably mainly because the contribution of skeletal muscle was lower. 3) Differences in the dietary protein level affected the utilization of dietary protein only a little, but influenced body protein metabolism very much.

Animals↗
PubMed1983

Nitrogen requirement of amino acid mixture with maintenance energy in young men.

The effect of energy on nitrogen balance was examined in young men given amino acid mixture. The minimum amino acid nitrogen requirement for nitrogen equilibrium was determined together with the egg protein requirement. In experiment 1, the nitrogen sparing effect of energy was evaluated in four male students receiving diet containing an amino acid mixture and a constant nitrogen intake of 3.5 g N/day, which was equivalent to the nitrogen requirement with excess energy intake determined by Rose and Wixom (1). When the dietary energy supply was 45 kcal/kg, which is approximately the maintenance level, the mean nitrogen balance was negative, being -23.9 +/- 9.3 mg N/kg. However, with an excess energy intake of 55 kcal/kg, the nitrogen balance improved significantly, being -6.1 +/- 7.7 mg N/kg. In experiment 2, the nitrogen requirement of egg-pattern amino acid mixture for apparently zero balance was evaluated at maintenance energy intake in 28 Japanese young men and was compared with that of egg protein. After receiving standard diet, the subjects were given a semi-purified experimental diet containing egg-pattern amino acid mixture at nitrogen intake levels of 60, 75, 100, and 130 mg N/kg for two weeks. Then all groups except the 60 mg N/kg group were given isonitrogenous egg protein diet for another week. Energy intake was kept constant at approximately the maintenance level of 44.4 +/- 1.4 kcal/kg throughout the experiment. Nitrogen balance was not significantly different in groups given egg-pattern amino acid mixture and intact egg protein in each nitrogen intake level. From regression analysis, the nitrogen requirement for nitrogen equilibrium of the amino acid mixture was calculated to be 110.1 +/- 50.2 mg N/kg, which was not significantly different from the value of 88.4 +/- 40.6 mg N/kg of egg protein. It was concluded that the total amino acid requirement estimated by Rose and Wixom (1) was too low because they gave excess energy, and that there was no difference between the nitrogen requirement of egg protein and that of the corresponding amino acid mixture.

Adult↗
PubMed1983

Effects of protein and energy on dietary selection and protein utilization in rats with ventromedial hypothalamic lesions.

The effect of bilateral destruction of the ventromedial hypothalamus (VMH) on protein and energy intakes was examined in relation to protein utilization. In Experiment 1, rats with VMH lesions were allowed to select protein (either casein or wheat gluten) and energy from two diets containing 70% and 5% protein, respectively. In Experiment 2, they were restricted to 0, 100 and 200 mg N/day of protein and given free access to protein-free diet. In Experiment 3, they were restricted to 2 and 5 g/day of protein-free diet but given 92% protein diet ad libitum. Self-selecting rats with VMH lesions consumed about 100 kcal/day regardless of the protein source, in contrast to sham-operated controls which consumed 75 kcal/day. They showed increased non-protein energy intake with constant protein consumption resulting in normal protein retention with increased body fat. When protein intake was restricted to 200 mg N/day, energy intake was reduced in animals given diet containing wheat gluten more than in those given diet containing casein, resulting in lower protein utilization. Therefore, food intake depended upon the amount of dietary protein. Rats with VMH lesions showed an exaggerated response to the ratio of protein to energy in the diet and consumed more energy from balanced diet but showed more reduction in food intake than controls in response to diet with an inappropriate ratio of protein to energy. The present study confirmed that the VMH was related to regulation of energy intake but not protein intake. It is suggested that protein intake is regulated in relation to maintenance of body protein in rats with VMH lesions as well as in normal rats.

Animal Feed↗
PubMed1983

Efficiency of utilization of soy protein isolate in Japanese young men.

The nutritional quality of soy protein isolate (SPI) was evaluated in young men by the nitrogen balance method using fish protein as a standard. Twenty-one male university students were given SPI (Supro 620, Ralston Purina Co., St. Louis, Mo., U.S.A.), fish protein (cod fish) or a 50:50 mixture of the two proteins as the sole source of protein. SPI and fish were prepared as paste products (kamaboko). Four experimental periods were used in which each subject received 0.35, 0.45, 0.55 and 0.65 g protein/kg/day, respectively, given in random order. Each period consisted of one day on protein-free diet and ten days on the experimental diet, with an interval of three days on a free-choice (ad libitum) diet between periods. Energy intake was constant for each individual to maintain their body weight (44.6 +/- 2.4 kcal/kg/day). The linear regression equations obtained between nitrogen intake (X: mg N/kg/day) and the apparent nitrogen balance (Y: mg N/kg/day) were as follows: SPI, Y = 0.298X-35.2; fish protein, Y = 0.365X-31.8; mixed protein, Y = 0.423X-38.3. The nitrogen requirement for maintenance of nitrogen equilibrium determined from the regression equation was 118.1 +/- 15.4 mg N/kg/day for SPI, 87.1 +/- 17.2 mg N/kg/day for fish protein and 90.5 +/- 17.1 mg N/kg/day for mixed protein. The NPUs calculated at the respective maintenance nitrogen intakes were 38.9, 52.8 and 50.8 for SPI, fish protein and mixed protein, respectively. There was no significant difference between the nutritive values of mixed protein and fish protein. The nutritive value of SPI relative to fish protein was estimated as 82%, 74% and 74% by the slope-ratio method, and values for the nitrogen requirement and NPU, respectively.

Adult↗