Biomedical subjects
Publications and source records attributed to G K Popov.
The study was performed on peripheral blood eosinophils isolated on a Ficoll-Verografin continuous-density gradient. Low-intensity laser radiation (lambda=0.89 mu, 25-30 mW power, 8 min exposure) induced degranulation of peripheral blood eosinophils. The effect of low-intensity laser radiation was realized via activation of calcium channels.
Expression of vascular endothelial growth factor was studied during neoangiogenesis stimulated by exposure of ischemic tissues in the limb muscles, liver, and myocardium and normal myocardial tissue to high-intensity laser radiation. Changes in expression were related to cell reactions during the inflammatory and reparative process and did not differ in the studied tissues.
Satellite cellular populations (mast cells, blood platelets and fibroblasts) were found to be the promoters in forming the new vessels. When irradiated by laser, the mentioned cells act as the main source for the angiogenic growth factors and matrix metalloproteinases, which comprehensively stimulate the proliferation and migration of endothelial and smooth-muscle vascular cells. Neo-angiogenesis, in response to the laser impact, is a stereotype, adaptive reaction of tissues to laser damage irrespective of a tissue type.
The paper describes the action of low-intensity laser radiation (LILR) and high-intensity one (HILR) on biological tissues. LILR produces its action on cells by changing the membranous formations-receptors and ion channels. At cardiac surgery, HILR stimulates uncontractile myocardial elements, by inducing the secretion of biologically active substances and the activation of enzyme systems that ensure the implementation of neoangiogenesis in the ischemic myocardium.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
The authors' findings evidence the possibility of the blood serum, liver, spleen, and bone marrow Fe, Cu, Mn, Co, Ni, and Zn measurements by the direct atomic absorption method without their separation and extraction. The technique is simple, meets the requirements to physicochemical analyses, and may be recommended for investigations in theoretical and practical medicine. The method is not devoid of a number of drawbacks, that limit the range of its applications; this concerns, among other things, Co measurements. The studies have demonstrated significant differences in the levels of trace elements in the tested biological material, which is explained by the species and organic characteristics of their metabolism.
The arachidonic acid metabolites, lipoxin B (5,14,15-trihydroxy-6,8,10,12-eicosatetraenoate) and 5,15-dihydroxy-eicosatetraenoate were shown to possess a marked effect on platelet aggregation and procoagulant activity. Lipoxin B increases and 5,15-dihydroxy-eicosatetraenoate decreases spontaneous platelet aggregation and procoagulant activity when added at various concentrations. The drugs tested were found to have no direct action on blood coagulation.
The lipoxin B (5, 14, 15-trihydroxy-6, 8, 10, 12-eicosatetraenoate) obtained by the enzymatic peroxidation of 5, 15-dihydroxy-6, 8, 11, 13-eicosatetraenoate (5, 15-DHETE) with the homogenous rabbit reticulocytes lipoxygenase and purified by HPLC stimulates the proliferation and differentiation of granulocyte-monocyte colony formation units (CFU-GMdc) of human peripheral blood at 3.6 X 10(-9) 3.6 X 10(-8)M in diffusion chamber placed in mouse abdominal cavity. The lipoxin B precursors namely 5,15-DHETE and arachidonic acid had stimulating influence at about 10(-6) and 10(-3)M respectively. The formation of polynuclear cells under the action of the studied lipoxygenase metabolites of the arachidonic acid was shown.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
In the experiments on Wistar rats to whom heparin was injected intravenously in the tail vein in doses of 50, 100 and 250 U, there were studied the colony-forming ability of the hemopoietic cells, the precursors of the bone marrow, and the colony-stimulating activity of the plasma of the heparinized animals 1, 3, 6, 12 and 24 hours after the drug administration. Heparin was found to increase the proliferative activity of the committed myelopoietic cells-precursors on a diffusion microchamber (GM-CFUdc) stimulating the mononuclears and elevating the level of the colony-stimulating activity of the plasma of the experimental animals.
The article describes the dynamics of colony-forming units in diffusion chamber of tubular bone marrow compartments 1 and 7 days after a burn. It was found that under normal conditions the activity of the axial part of the bone marrow is twice that of the cortical part. After a burn the inductive capacity of the cortical part becomes equal to that of the axial part. A wider qualitative spectrum of colonies occurs due to the effect of short-distance humoral factors (eosinophils, mast cells).