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Biomedical subjects

G Klima

Publications and source records attributed to G Klima.

At least 109 records · Page 6Linked to original sources
PubMed1988

[Changes in zinc level in the serum, whole blood and erythrocytes in disorders of thyroid function].

The zinc level in serum and whole blood was determined in a series of 121 patients, 20 suffering from overt hyperthyroidism, 34 with manifest hypothyroidism and a control group of 67 euthyroid subjects. The zinc content per deciliter of erythrocytes was calculated from the haematocrit. The hyperthyroid group revealed a highly significantly elevated serum zinc level (p less than 0.01), but a most significantly reduced zinc content in whole blood (p less than 0.001) in comparison with the euthyroid group. On the other hand, the reverse changes were observed in the hypothyroid group, i.e. a most significantly reduced serum zinc content (p less than 0.001) and a highly significantly increased zinc content in whole blood (p less than 0.01). These results suggest that the determination of zinc levels both in serum and in whole blood may be a useful additional parameter of peripheral thyroid hormone effect.

Erythrocytes↗
PubMed1988

[The negative TRH test: new differential assessment using a sensitive TSH assay].

Determination of TSH by the IRMA technique shows improved sensitivity and higher accuracy compared to TSH RIA, so that lower detection limits of 0.1 mU TSH/1 can be easily attained. Basal TSH values (bTSH) between 0.4 and 3.5 mU/l clearly define a euthyroid metabolic state. At a bTSH lower than 0.4 mU/l a TRH test should be performed to exclude latent or overt hyperthyroidism. Stimulated TSH values (sTSH) greater than 2.5 mU/l are considered to be a positive test which rules out hyperthyroidism. A negative TRH test (sTSH less than 2.5 mU/l), on the other hand, is an abnormal result which may have several causes and thus does not per se evidence a hyperthyroid state. In a group of thyroid inpatients, a differential new assessment of the so-called negative TRH test for the exclusion of hyperthyroidism was attempted in 355 patients with blunted TSH response (sTSH less than 2.5 mU/l) by means of a methodologically improved TSH assay (IRMA principle). In overt hyperthyroidism the bTSH was lower than 0.1 mU/l in 42 out of 44 cases (95%); sTSH was in all cases below 0.5 mU/l (100%). 78% of the clinically euthyroid patients had a blunted bTSH (0.1-0.4 mU/l) and a significant (p less than 0.001) increase of sTSH up to 0.5-2.5 mU/l. Excluding the patients treated with L-thyroxine, 147 cases had sTSH less than 2.5 mU/l. Among the 65 patients with sTSH ranging between 0.5 and 2.5 mU/l, 66% were euthyroid and 34% borderline cases from a clinical point of view (mostly compensated autonomous adenomas), but there were no hyperthyroid cases.(ABSTRACT TRUNCATED AT 250 WORDS)

Diagnosis, Differential↗
PubMed1988

Hypothyroidism and the influence on human blood rheology.

Viscoelastic properties of human whole blood were measured in 338 subjects: 110 persons (75 F, 35 M) following total thyroidectomy but without substitution therapy over three weeks as well as 228 healthy control subjects (124 F, 104 M) were investigated in this study. Subjects with other variable factors were excluded. The viscous and the elastic portion of apparent whole blood viscosity -the latter subdivided into red cell aggregation and red cell rigidity were determined by using a newly developed oscillating capillary rheometer and densitymeter at the real hematocrit value and at a computed hematocrit of 40%. Serum viscosity, hematocrit (Hct), hemoglobin (Hb), mean corpuscular volume of red blood cells (MCV), free triiodothyronine (fT3), free thyroxine (fT4) and serum-thyrotropin (bTSH) were evaluated as well. In a further subgroup of 80 subjects (40 patients with hypothyroidism, 40 healthy control persons) additional plasma viscosity and sedimentation rate were determined. Red cell aggregation and blood viscosity especially at shear-rates below 10/sec increased significantly in hypothyroid patients. There was no significant difference of red cell rigidity, serum viscosity, plasma viscosity and sedimentation rate as against euthyroids. So new aspects of thyroid dysfunction could be described probably with consequences for therapy and for considerations about the development of atherosclerosis and ischemic disorders.

Adult↗
PubMed1988

[Immunohistochemical studies of the nature of connective tissue in fetal membranes].

Fetal membranes at the end of pregnancy have been examined indirectly with the help of FITC labelled antibodies for the occurrence of different collagen types (I, II, III, IV) and their initial steps as well as fibronectin and laminin. The connective tissue of amnion and chorion is composed of collagen type I and p-III. Collagen type IV occurs only rarely. Despite of the borderline reticulin fibres are not the component of these mechanical resistant fibre-mats. In the PAS positive material, seen between the cells of the trophoblast, laminin and type IV collagen were found. It can be stated that the compound of the intercellular substance is of the same kind as the basement membrane.

Amnion↗
PubMed1988

[The acute hemorheologic effect of calcium dobesilate].

In a series 40 subjects without manifest vascular disorders received 1000 mg Calcium dobesilate (Doxium). Before and 3 hours after application the viscoelasticity of whole blood as well as plasma viscosity and the hematocrit were determined. The statistical analysis was performed by Wilcoxon-test. The viscosity of whole blood and the hematocrit decreased with a significance of p less than or equal to 0.001, whereas the elasticity decreased in a significance manner of p less than or equal to 0.01. Plasma viscosity remained uncharged. The results show an effect of hemodilution, which could be very important for treatment of hyperviscosity.

Adult↗
PubMed1988

Calcium dobesilate and its effects on hemorheology and microcirculation.

In series, 50 patients suffering from cerebral (n = 33) and peripheral (n = 17) diseases were treated with 3 x 500 mg calcium dobesilate (Doxium) per day. Before onset of therapy and after 14 days the viscoelasticity of whole blood and plasma as well as hematocrit and microcirculation at the finger pad were measured. Viscoelasticity of whole blood, plasma and hematocrit decreased in a significant manner (p less than or equal to 0.001), while microcirculation increased significantly (p less than or equal to 0.001). The results justify the application of calciumdobesilate for ischemic diseases with elevated viscosity.

Adult↗
PubMed1986

[Cytologic studies of the aqueous humor to determine immune reactions].

Although corneal grafting is performed on a so-called privileged (i.e., unvascularized) tissue, it is not always successful. Depending on the degree of vascularization of the host cornea (Stages I-IV) allograft reactions occur in 3 to 70% of patients thus treated. In an animal experiment (rabbits) a graft reaction was produced in the anterior chamber of one eye following presensitization with soluble corneal proteins, after implanting corneal tissue in the anterior chamber under the protection of a viscoelastic substance. The same grafting procedure was repeated on the fellow eye without previous sensitization. In the course of the immunoreaction the cell content of both eyes was studied and, 14 days after grafting, the result of aspiration cytology was compared with findings from light-microscopic examinations of the corneal graft and host cornea. A correlation was established between the results of aqueous humor cytology and the cytological findings in the corneal graft. It follows that immunoreactions can be interpreted reliably by aqueous humor aspiration cytology.

Animals↗
PubMed1986

[Visual fixation tonometry as an aid in the early diagnosis of endocrine ophthalmopathy].

In some cases of autoimmunogenic hyperthyroidism an endocrine ophthalmopathy is found, but it is difficult to diagnose Graves' disease in early stages. Even before the occurrence of measurable morphological changes (measured by sonography and computed tomography of the orbit and eye muscles) short positional intraocular pressure changes are found in 64.5% of cases with autoimmunogenic dysthyreosis. These pressure changes are detected by positional applanation tonometry. These findings admit the conclusion that with the method described not only early detection of ocular signs associated with thyroid disease is possible, but also early differentiation between autoimmunogenic and nonimmunogenic thyroid disorders.

Eye Movements↗