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Biomedical subjects

G Lanzer

Publications and source records attributed to G Lanzer.

At least 55 records · Page 3Linked to original sources
PubMed1986

[Optical immunofluorescence detection of alloantibodies as a supplement to classical crossmatching within the scope of organ transplantation].

The importance of the indirect immunofluorescence test in evaluating an alloantigen-sensitization in organ transplantation is examined. In some antigen-antibody combinations, which are non reactive in standard complement dependent microlymphocytotoxicity assay there is seen a highly positive reaction in the indirect immunofluorescence test. Therefore this test is an important supplementation in the compatibility-definition in organ transplantation.

B-Lymphocytes↗
PubMed1986

[Cyclosporin A as a diabetogenic cofactor].

Two kidney transplanted patients are reported, who developed an insulin dependent diabetes mellitus after crossing therapeutic Cyclosporine A levels. After stabilisation of the Cyclosporine A levels the insulin dependent diabetes mellitus was completely reversible. The results are indicating Cyclosporine A as the insulin dependent diabetes mellitus initiator.

Adult↗
PubMed1986

[Therapeutic separation of blood components in clinical emergency medicine].

In this paper the clinical application of component separation by means of the cell separator is demonstrated. Patients with acute intoxications, thyroid storm, plasmatic and cellular hyperviscosity syndromes, immuno-complex- and antibody mediated diseases are shown to be successfully treated on the cell separator. It is shown that the cell separator is a practicable contribution to internal medicine in the subdisciplines of clinical immunology, hematology and intensive care medicine.

Adult↗
PubMed1985

[Immunologic and transplantation biology studies in patients with terminal renal failure].

Best possible tissue compatibility is a fundamental precondition for a successful organ transplantation. The desirable tissue compatibility is mainly defined by transplantation-antigens of the donor and the recipient and therefore--beside ABO antigens--the characteristics of HLA-system are the focal point of the preoperative immunological diagnosis. Based on the results of comperative examinations it is demonstrated, that HLA-antisera have such a widespread biological variability, that they lead sometimes to a faulty diagnosis which in turn causes the biological importance of the HLA-system to be doubted. The preoperative immunologic diagnosis should include an estimation of the risk factors in the patient. The consideration of the crossmatch between donor and recipient is a decisive factor in organ transplantation. The starting point is the preoperative antibody monitoring which checks the patients serum reactions against a panel of blood donors to see, whether the patient is a "high" or "low"-- responder to allogenetic stimuli. A positive reaction in the crossmatch is brought about by different kinds of antibodies whereby only in the presence of auto-antibody or cold-reactive B-cell antibody a transplantation may take place. The antibody characterisation in preoperative diagnosis is supported by the results of the immunologic antibody monitoring, whereby--because of the results here presented-- it can be confirmed, that through the knowledge of the antibody specifities which have been checked in a positive crossmatch transplantation on highly sensitized patients can take place with a prospect of success. Supplementing the "Eurotransplant" results the HLA-DRw6 antigen is shown not only to be an indication of risk in transplantation, but may also be an aetiopathogenetic factor. In the presentated statistics it can be shown, that in patients suffering from glomerulonephritis or pyelonephritis and requiring dialysis treatment the HLA - DRw6 antigen occur more frequently than in the control group of healthy blood donors. In glomerulonephritis patients there is additionally a significant change in distribution favourable to HLA - DRw10 shown. The determination of genetically caused risk factors is appreciably supported by characterisation of lymphocyte subpopulations and diagnosis of changes in the Complement-system. Changes in T-lymphocyte subpopulations pointing to proceedings of immunostimulation and conditions of the activated Complement-system represent warning signals in organ transplantation.

Adult↗
PubMed1983

[Primary biliary liver cirrhosis].

Primary biliary cirrhosis, or chronic destructive nonsuppurative cholangitis, is a condition of chronic cholestasis, in which small intrahepatic bile ducts in the portal zones of the liver become progressively destroyed. The etiology of primary biliary cirrhosis is unknown, but the observation of (a) mitochondrial antibody, (b) elevated serum levels of IgM and (c) circulating immune complexes and (d) impaired lymphocyte transformation (- cooperation) strongly suggest, that disordered immune responses play a major role in the initiation or progression of this chronic hepatic lesion.

Alkaline Phosphatase↗
PubMed1983

[Clinical application of alkaline phosphatase bone-isoenzyme determination with acetate foil].

Clinical application of alkaline phosphatase bone isoenzyme determination using acetate foil is investigated in patients with known underlying disease and is compared with diagnostic evaluation of 112 bone-scans. The separation of alkaline phosphatase isoenzymes proves a valuable diagnostic support of bone scintigraphy. Isoenzyme determination moreover reveals primary and secondary bone changes in early stages of the disease and also offers the possibility of quantitative follow-ups.

Adenocarcinoma↗
PubMed1982

[Diagnostic problems in monoclonal gammopathies].

This is a report on 123 patients with monoclonal gammopathies at Graz University Medical School. Approximately 75 percent of them were malignant, the rest benign or unclassified. In view of the considerable interest for differential diagnosis benign versus malignant, the criteria for both are given.

Antibodies, Monoclonal↗
PubMed1982

Defects of leukocyte locomotion in multiple myeloma.

23 patients with multiple myeloma of IgG, IgA and light chain type, one patient with benign monoclonal IgE gammopathy and 23 healthy controls were investigated for their leukocyte locomotion (LL), for the ability of their sera to generate chemotactic factors upon activation with zymosan, and for inhibitory effects of their sera upon LL. While no significant impairment of the patients' LL was observed when casein was used as cytotaxin, LL was markedly reduced in myeloma patients with zymosan activated control serum as chemoattractant. The ability of serum to generate chemotactic factors upon activation with zymosan was similar in patients and controls, but preincubation of control leukocytes with sera derived from patients with multiple myeloma caused a significant inhibition of LL (p less than 0.001). This LL-inhibitory serum effect was not correlated with the type of paraproteinemia. We conclude that the impairment of LL in multiple myeloma and the LL-inhibitory serum effect might contribute to the increased susceptibility to infections in that disorder.

Caseins↗
PubMed1981

Concanavalin A-induced suppressor cell activity and in vitro immunoglobulin secretion in rheumatoid arthritis: correlation with clinical and immunological parameters.

Concanavalin A-induced suppressor cell activity was found moderately but significantly (P less than 0.05) decreased in RA patients treated with nonsteroidal antiinflammatory drugs (31 +/- 7% suppression) as compared to patients on remission-inducing drugs, such as gold, penicillamine, or chloroquine (51 +/- 6%) or to healthy individuals (50 +/- 6%). Also, lymphocytes from patients with antibodies to collagen mediated lower suppression (33 +/- 7%) than lymphocytes from patients without evidence for these autoantibodies (61 +/- 11%). No significant difference between patients and controls or between individual groups of patients were observed in regard to IgM and IgG secretion induced by pokeweed mitogen. Thus, although no indication for a severe derangement of regulatory cells in peripheral blood of RA patients could be observed in this study, a slight deficiency of ConA-inducible suppressor cells that may be reverted by remission-inducing drugs seems to be present in RA.

Adult↗
PubMed1981

[Mechanisms of action and clinical importance of C3-nephritic-factor (author's transl)].

The C3-nephritic-factor is an autoantibody specific against C3-convertases. This IgG-immunoglobulin is particularly found in patients suffering from membranoproliferative glomerulonephritis and causes a complement-activation under elimination of physiological regulator mechanisms. The clinical importance of this factor is discussed.

Complement C3 Nephritic Factor↗
PubMed1981

[Cell hybridization: a monoclonal marker as a diagnostic help in haematology and oncology (author's transl)].

This is a communication on the introduction of the first monoclonal marker at Graz University Medical School. Human peripheral blood mononuclear cells were used for immunisation of BALB/c mice by injecting 4,5 X 10(6) cells s. c. Boosting consisted of i.p. injection of 5 X 10(6) cells 4 times in monthly intervals. Spleen cells were taken 4 days after the last boost, fused with NS-1 myeloma cells, using PEG as fusogenic agent. After growth in HAT selective medium, antibody secreting clones were identified by testing the supernatants. Cultures with activity against lymphocytes were closed on normal BALB/C peritoneal cell feeder layers. One of them secreted IgG 1 with strong activity against all lymphoid cells and was named HLy D 1. Further testing showed activity with band cells, polymorphs, eosinophils, macrophages but not with tissue sections from anaplastic undifferentiated cancers and erythroid leukaemias. Since he was named H Le D 1 and introduced for differentiating rare undifferentiated carcinomas from malignant tumors of the lymphoid system.

Animals↗