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Biomedical subjects

G P Adamenko

Publications and source records attributed to G P Adamenko.

8 recordsLinked to original sources
PubMed1979

[Replacement of the helper function of T cells by an RNA-containing antigen-specific lysis factor].

The lymph node viable cells suspension of immunized mice was centrifugated. The supernatant was chromatographed in Sephadex G-200, and fractions were deproteinized. The deproteinized third fraction (Mol wt 30000) stimulated specifically the plaque-forming cells of intact mice immunized by SRBC. It restored the capacity to antibody production in the lethally irradiated intact mice protected by the syngeneic bone marrow. The activity of this fraction disappeared following treatment with RNA-ase, but not with DNA-ase or trypsin. The first and the second deproteinized fractions of the supernatant inhibited non-specifically the viable lymph node cells of the immunized animals in the intact mice immunized with SRBC.

Animals↗
PubMed1976

[Antigen-specific lysis in vitro of lymph node cells of intact mice injected with RNA from viable lymph node cells of immunized animals].

Supernatant fluid obtained after centrifugation of the suspension of viable lymph node cells of immunized animals proved to induce in vivo in the lymph node cells of intact mice sensitivity to lysis with a specific antigen in vitro. This property was possessed after chromatography of the supernatant fluid on Sephadex G-200 by the 3rd fraction (MW about 30000 dalton). DNA-ase, trypsin or deproteinization failed to influence whereas RNA-ase inactivated this fraction in respect to the inducing properties.

Animals↗
PubMed1976

[Immunochemical characteristics of a factor causing antigen-specific lysis of thymus-dependent lymphocytes].

A factor capable of lysing in vitro, in the presence of a specific antigen, the cells of the lymph nodes and the thymus of intact mice was revealed in the supernatant obtained after the centrifugation of a suspension of viable cells of the lymph nodes and the thymus of the immunized mice. It was found by immunochemical methods that this factor had a mol. wt. of about 30000 dalton and an electrophoretic mobility in polyacrylamide gel exceeding that of mouse blood serum albumin. Besides, it was revealed by the precipitation reaction in agar that it was not an immunoglobulin or its chains.

Animals↗
PubMed1976

[Study of bacterial allergy in acute appendicitis by the method of leukocyte migration inhibition in vitro].

Under study was the effect of various bacterial allergens on the migration of blood leucocytes from capillaries in vitro in 23 patients with acute appendicitis and in 18 healthy individuals. Bacterial allergens wound suppress leucocytes migration in all patients. Most frequently, sensibilization to allergens of hemolytic staphylococcus and hemolytic streptococcus was found. In 4 healthy persons this methods indicated sensibilization to some allergens. The interaction of activity sensibilized lymphocytes and passively sensibilized polynuclears of the appendix with bacterial antigens is assumed to tigger the hyperergic reaction in acute appendicitis.

Acute Disease↗
PubMed1976

[Determining hypersensitivity to antigens by computing cells migrating from capillary tubes].

The test technique of inhibition of leukocyte and macrophage migration from the capillaries in the presence of a specific antigen is described. The method is highly sensitive and permitted to use a small amount of cells and many (up to 600 and more) capillaries. Sodium azide, phitohemagglutinin and incubation at 4 degrees C inhibited the cell migration from the capillaries. Dead cells did not pass from the capillaries into the medium. Tuberculin and BCG vaccine inhibited the migration of macrophages of guinea pigs immunized with BCG, but not with staphylococcus allergen.

Animals↗