[Antigen-specific lysis in vitro of lymph node cells of intact mice injected with RNA from viable lymph node cells of immunized animals].

G P Adamenko; D K Novikov

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PubMed · 58684

[Antigen-specific lysis in vitro of lymph node cells of intact mice injected with RNA from viable lymph node cells of immunized animals].

G P Adamenko·D K Novikov

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Abstract

Supernatant fluid obtained after centrifugation of the suspension of viable lymph node cells of immunized animals proved to induce in vivo in the lymph node cells of intact mice sensitivity to lysis with a specific antigen in vitro. This property was possessed after chromatography of the supernatant fluid on Sephadex G-200 by the 3rd fraction (MW about 30000 dalton). DNA-ase, trypsin or deproteinization failed to influence whereas RNA-ase inactivated this fraction in respect to the inducing properties.

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Animals Epitopes Female Immunization In Vitro Techniques Lymph Nodes Male Mice Mice, Inbred Strains RNA English Abstract Journal Article

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G P Adamenko, D K Novikov. 1976. [Antigen-specific lysis in vitro of lymph node cells of intact mice injected with RNA from viable lymph node cells of immunized animals].. https://pubmed.ncbi.nlm.nih.gov/58684/

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