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Biomedical subjects

G Pasternak

Publications and source records attributed to G Pasternak.

At least 37 records · Page 2Linked to original sources
PubMed1988

Raji-K562 hybrids and their use for trioma production.

Cells from the B lymphoblastoid cell line Raji (Burkitt lymphoma) and from the myeloid-erythroid cell line K562 (subline AB5), stained by two different fluorochromes, were fused in vitro by means of PEG. Hybrid cells exhibiting heterofluorescence were isolated in a cell sorter (FACS III). They were cloned and analyzed for both Raji and K562 derived antigens. Hybrid cells retained the excellent growth behaviour of AB5 cells while expressing also Raji antigens. One of the hybrids (BD7) was used for trioma production when fused with human stimulated spleen cells. Trioma cells representing a fusion product of three parental cells may be useful for studying problems of gene regulation and expression.

Antibodies, Monoclonal↗
PubMed1988

Immunoblotting of monoclonal anti-cytoskeletal antibodies with cytoskeletal extracts of HeLa and MCF-7 cells.

Cytoskeletal extracts of HeLa and MCF-7 cells were separated by SDS polyacrylamide gel electrophoresis, electrotransferred to nitrocellulose paper and exposed to five of our monoclonal antibodies (mabs) against structures of the cytoplasm as demonstrated in indirect immunofluorescence. Three of them reacted with cytokeratins according to histological evidence. All mabs tested in this study were reactive in immunoblotting. The broad-range anti-cytokeratin antibody A45-B/B3 was shown to react with cytokeratin polypeptides 8 and 18 and possibly 7, but not with cytokeratin 17.A53-B/A2 bound only to cytokeratin 19.Mab A 51-B/H4 showed a reaction with cytokeratin 18. Two mabs not yet described in detail (A45-C/H1 and A45-C/H9) reacted with a single band of 25 kD and may detect a cytoskeleton-associated protein.

Antibodies, Monoclonal↗
PubMed1987

Fulminant hepatic failure after occupational exposure to 2-nitropropane.

Two construction workers became ill after applying an epoxy resin coating containing 2-nitropropane in the confined space of an underground concrete vault. One man died 10 days later from fulminant hepatic failure. The second man recovered but has had persistently elevated serum aminotransferase activity. The serum concentration of 2-nitropropane on admission of the man who died was 13 mg/L, and was 8.5 mg/L in his coworker. The acute toxicity of 2-nitropropane has not been widely appreciated despite previous reports of death due to hepatic failure after exposure to the compound in confined spaces. These cases show the importance of effective education and protective work practices.

Adult↗
PubMed1985

[Lymphocyte reactivity against fetal antigens during the growth and regression of experimental tumors].

Sensitization of lymphocytes occurs to fetal antigens during growth of malignant tumours. Sensitization was measured by the MEM-technique. Antigenic preparations, obtained by extraction with 3 M KCl of mouse fetal tissues were used. Growing tumors after allogenic transplantation of tumour cells gave rise to cellular sensitization. The disappearance of the tumours resulted in a decrease of the sensitization status. Lymphocytic sensitization to fetal antigens was evident in mice with intradermally growing tumors, too. After ligation, followed by regression of the tumours the sensitization became no longer detectable. The results show that tumour regression is associated with reversibility of cellular sensitization to fetal antigens.

Animals↗
PubMed1985

[Lymphocyte reactivity of tumor-bearing mice to a 3M KCl extract of fetal mouse tissue: removal of diffusion chambers containing tumor cells reverses the reactivity].

Sensitization of lymphocytes to 3M KCl fetal extracts was shown by the MEM test after diffusion chambers containing tumor cells have been implanted into mice. Lymphocyte reactivity was still present 2, 4 and 6 weeks after removal of the chambers. When tested after 9 and 12 weeks the sensitization was no longer detectable. This results show the reversibility of sensitization after complete removal of tumor cells.

Animals↗
PubMed1984

Lymphocyte reactivity in normal and malignant cell proliferation against a phylogenetically conserved antigen in fetal extracts.

Lymphocyte reactivity to 3-M KCl extracts from fetuses of different species of origin was shown by the macrophage electrophoretic mobility (MEMB) and/or the leukocyte migration inhibition tests in tumor-bearing mice and humans. In chemical carcinogenesis, reactivity was detectable before tumors developed. Six weeks after sc injection of 1,000 micrograms benzo[a]pyrene [(BP) CAS: 50-32-8] into XVII/Bln mice, the MEMB test became positive. The latent period was 15 weeks after 1.0 micrograms BP, indicating a dose-response relationship of the phenomenon. Painting of mouse skin with 7,12-dimethylbenz[a]anthracene [(DMBA) CAS: 57-97-6], croton oil (CAS: 8001-28-3), or benzene (CAS: 71-43-2) had the same sensitizing effect as BP. In contrast to the strong carcinogens BP and DMBA that caused lymphocyte reactivity to persist until tumors developed, benzene and the promoter croton oil induced only a transient effect. Termination of treatment abolished reactivity within 12 weeks. Lymphocyte reactivity to fetal extract in normal cell proliferation was evident from the fact that two-thirds-hepatectomized rats and BCG-treated mice became MEMB-positive. In hepatectomized rats the effect was reversible according to the completion of liver regeneration. Lymphocytes from tumor-bearing mice reacted with mouse and human fetal extract as well as with extracts from different developmental stages of frogs and fish. Fetal extracts were assumed to contain a phylogenetically conserved antigen.

Animals↗
PubMed1983

[Cross-reactivity of 3M KCl extracts of fetal tissue from various species in the macrophage electrophoresis mobility (MEM) test. I. Extracts from fetuses of mammals and one species of fowl].

Extracts were prepared by the 3M KCl method from fetal tissues of human, mouse, cattle, pig, sheep, cat, guinea pig, and chicken origin. Lymphocytes of tumor bearing mice and tumor patients reacted specifically in the MEM-test with antigenic components of the extracts. The cellular sensitization of the tumor-bearer was not correlated with the localization and the histologic type of the tumor. The cross-reacting oncofetal antigens obviously represent phylogenetically conserved structures.

Animals↗
PubMed1983

[Detection of cellular sensitization in mice after treatment with chemical carcinogens].

Mice were treated epicutaneously (DMBA, benzene) and subcutaneously (BP, MNH) with chemical carcinogens. As detected by the MEM-test a cellular sensitization developed against fetal antigens, which are present in 3M KCl-extracts of mouse fetal tissue. The sensitization was detected before formation of tumors occurred. The conclusion was drawn that sensitization to fetal antigens is not a characteristic feature of tumor-bearing animals.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1983

[Cross-reactivity of 3M KCl-extracts of fetal tissue from various species in the macrophage electrophoresis mobility test. II. Tissue extracts from lower vertebrates in various phases of development].

Cellular sensitization was detected by the MEM-test using 3M KCl-extracts of fetuses from man and mouse and of different metamorphic stages of the water frog (tadpoles) and the carp (yolk sack-bearing stage). The sensitization demonstrated was independent on the type of tumor tested. The occurrence of active components in the extracts was correlated with certain stages of individual development of water frog and carp. The evidence of these components in species of several classes of vertebrates confirms the assumption of phylogenetically conserved structures.

Animals↗
PubMed1983

[Cellular sensitization to fetal antigens during chemical carcinogenesis].

Tumor-bearing mice show a cellular sensitization to fetal antigens as detected by the macrophage electrophoretic mobility (MEM) test. Sensitization is independent of etiological and histological tumor factors. An identical state of sensitization can be found after application of chemical carcinogens (N-methyl-N-nitrosourea, benz(a)pyrene or 7,12-dimethylbenz(a)anthracene) before the tumors have developed. The results indicate that the effect is dose-dependent.

Animals↗
PubMed1982

Reactivity in neoplasia, preneoplasia, and pregnancy of lymphocytes against fetal extracts: cross-reactions between man and mouse.

KCl extracts (3 M) from human fetuses were tested by macrophage electrophoretic mobility (MEMB), leukocyte migration inhibition, and/or leukocyte adherence inhibition techniques against lymphocytes-leukocytes from tumor-bearing humans and control persons. A positive MEMB test was found in 209 of 284 (73%) patients with various types of clinically manifest tumors. Only 15 of 134 (11%) controls gave a positive reaction by the MEMB test. Leukocyte migration was significantly inhibited in 48 of 64 (75%) tumor patients, and leukocyte adherence was significantly inhibited in 40 of 50 (80%) tumor patients versus 7 of 50 (14%) and 10 of 54 (19%) in the corresponding controls. Lymphocyte reactivity to fetal extracts is also detectable in inbred XVII/Bln, CBA/Bln, and C3H/Bln mice bearing spontaneous or transplanted tumors of different etiology. Human and mouse fetal extracts gave cross-reactions. Lymphocytes from tumor-bearing mice were reactive against fetal extracts from the cow, pig, sheep, guinea pig, cat, and chicken. Mice bearing benign skin warts induced by 7,12-dimethylbenz[a]anthracene (DMBA) as well as DMBA-treated mice without visible skin lesions gave a positive MEMB test. Likewise, pregnancy is associated with lymphocyte reactivity to fetal extracts.

Animals↗
PubMed1982

The leukocyte adherence inhibition test in tumor patients using 3M KCl extracts of fetal tissues as antigens.

The sensitization of tumor patients was evaluated with the LAI test to 3M KCl extracts of human fetuses derived from abortion in the 1st-3rd month, 5th month and 7th month of pregnancy. Positive responses were seen against preparations from fetuses at different developmental stages: reactivity was found among 12/18 (1st-3rd month), 13/15 (5th month) and 14/16 (7th month) of the tumor patients. In total, 80% (39/49) of the patients were sensitized to fetal extracts. In contrast, only 19% (10/54) of the control group reacted positively in the LAI test.

Antigens↗
PubMed1981

The present status of immunodiagnosis and immunotherapy of cancer.

A number of immunodiagnostic procedures in cancer is clinically important in the sense that the tests may confirm the results of other cancer diagnostic investigations. However, each one of the tests has its own area of application. Immunodiagnostic tests are used for the detection of relapse or metastases as well as for the histological verification of the tumor. A mass screening for tumors in the population and most probably the early tumor diagnosis by immunological means is impossible due to biological variables among the tumor bearers and to technical difficulties. Immunotherapy of tumors is still at an experimental stage. Although immunization is possible to animal tumors convincing data on similar effects in the human tumor system do not exist as yet. This conclusion is based on immunization experiments with adjuvants as well as with tumor preparations, transfer factor, thymosin, or immune RNA. The fact that tumor cytotoxic and tumor cytostatic immunological mechanisms do occur even in the human tumor system is a stimulus for future approaches.

Animals↗
PubMed1981

Cellular sensitization in man and mice during pregnancy to fetal antigens as detected by lympholine assays.

Soluble extracts were prepared by the 3 M KC1 method from human mouse fetuses at different stages of development. As shown by macrophage electrophoretic mobility and by leukocyte migration inhibition tests a high percentage of pregnant women is sensitized to components of these extracts. The testing of pregnant mice of an inbred strain gave similar results. The available data support the view that some kind of cellular sensitization to fetal antigen may occur during pregnancy.

Animals↗
PubMed1980

[Immunodiagnosis of cancer: requirements and results (author's transl)].

Theoretical aspects are considered on the requirements of immunological cancer tests. The criteria of clinical applicability are: Specificity, sensitivity, information with respect to tumour localisation. Immunodiagnostic tests cover the following areas: Early diagnosis on the basis of screening of the whole population or of risk groups, differential diagnosis, detection of relapse or metastases, prognosis. Experimental results are presented on a test for tumour detection. In a blind test study patients with clinically manifest tumours were recognized in about 80--90%, when using human fetal extract as antigen in the macrophage-electro-phoretic-mobility technique.

Antigens↗