After passing normal mouse spleen cells through columns of a weakly basic ion-exchanger, the percentage of cells with B cell-typical electrophoretic mobility (EPM) is 90% after fractionation at pH 8.0 and 10--20% at pH 5.0. However, the characterization of fractionated cells with regard to theta-antigen as well as graft-versus-host reaction shows that separation of T- and B-lymphocytes had not occurred. After incubation of the separated cells for 2 h at 37 degrees C the EPM returned to that of unseparated cells. It appears that the net charge of T- and B-lymphocytes is changeable depending on pH, temperature and separation material.

Animals↗

PubMed1978

[Detection of tumor-associated cell membrane antigens of UV-induced mouse sarcoma by the ferritin-hybrid antibody method].

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Animals↗

PubMed1978

[Leukocyte migration inhibition and leukocyte adherence inhibition (LMI- and LAI-assay) in cancer patients by using preparations from human fetuses].

The sensitization of lymphocytes from patients with different tumors was tested against a 3 M KCl-extract of fetal tissue (3.--6. month) by the leukocyte adherence inhibition assay (LAI) and by the leukocyte migration inhibition assay (LMI). Sensitization was compared with the reactivity of controls without any detectable tumor. In the LAI assay the leukocytes of 13/15 patients and 2/12 controls showed an inhibition of the adherence. In the LMI-assay 11/17 tumor-bearing patients and 6/18 controls reacted positive in the presence of the antigen preparation. The two methods demonstrated that patients bearing tumors of different histology are sensitized to fetal antigens.

Animals↗

PubMed1978

A common differentiation antigen on plaque forming cells and a subpopulation of thymus cells in mice and rats.

A non species specific lymphocyte differentiation antigen is described which can be detected on plaque forming cells and a small fraction of thymocytes of mice and rats. The antigen is absent from a BALB/c plasma cell tumor and is not detectable on Dexamethasone-resistant thymocytes. On the basis of its occurrence the term TPCA (thymus plasma cell antigen) is proposed for the antigen. A monospecific anti TPCA serum could be prepared which enables the detection of the antigen on about 10% of rat thymocytes.

Animals↗

PubMed1977

[Coronary arteriosclerosis and risk factors in coronary heart disease (author's transl)].

A large number of cases were tested for the relevance of clinical risk factors in coronary heart disease with regard to coronary arteriosclerosis (atheroma, fibrosis; five different locations). The method applied was VARIMAX-rotated factor analysis. The resulting factors were interpreted as the multiple variables structures which are in different relationships to the risk criterion, coronary heart disease, and morphological vascular diagnosis of the coronary arteries. The importance of the known risk of the vascular diagnosis was estimated and the reterance of the clinically influencing criterion was discussed. It appears that in spite of limited methodology additional morphological criteria are required in order to be able to explain heart infarction which does not involve the epicardial branches of the coronary vascular system.

Adult↗

PubMed1977

Lymphokines of T and B cells. I. Demonstration that T and B cells of tumor antigensensitised mice produce different lymphokines changing surface charge of target cells.

Lymphocytes from MC sarcoma-bearing mice were exposed to a soluble tumor antigen. The lymphokine-containing supernatant thus obtained was fractionated on Sephadex G-75. Fractions were tested for their activity to reduce the surface charge of indicator macrophages by cell electrophoresis technique. When unseparated spleen or lymph node cells were used the charge-reducing activity of the supernatant was found in three regions according to molecular weights of about 100,000, 60,000 - 35,000 and 13,000 Daltons. The appearance of activity was found to be time-dependent. As indicated by tests with separated lymphocytes both B and T cells appear to release charge-reducing activity.

Animals↗

PubMed1977

Pancreatic ascites: diagnosis of leakage site by endoscopic pancreatography.

A case with pancreatic ascites is presented in which the site of leak from a pancreatic pseudocyst was identified by oral endoscopic pancreatography. It is suggested that this procedure be done early in the clinical course of most patients with pancreatic ascites, since the information obtained will facilitate medical and surgical management.

Adult↗

PubMed1976

Cellular immunity in mammary cancer patients as measured by the leukocyte migration test (LMT). A follow-up study.

In 58 cases of mammary cancer treated by surgery the leukocyte migration test (LMT) has been applied to the study of cellular immunity using homogenate from autochthonous and homologous tumors as antigens. A positive test, i.e. inhibition of migration by antigen, was observed in 52 patients from 1 day up to 40 days after surgery. Six patients were negative. There was extensive immunological cross-reactivity among mammary cancer in that a positive test was also obtained with homogenate from homologous mammary tumors. Generally, antigens from other tumors did not react. The test was also negative when the leukocytes were derived from healthy persons. Sera from mammary cancer patients abrogated the inhibitory effect of tumor antigen. The disappearance of positivity about 40 days after surgery and its reappearance during the metastatic process indicates that the positive LMT is associated with progressive disease.

Adult↗

PubMed1976