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Biomedical subjects

G R Krueger

Publications and source records attributed to G R Krueger.

At least 55 records · Page 3Linked to original sources
PubMed1988

Diffusion of HIV-1 virus into non-habitual categories at risk in European countries.

The ECP Working Group on AIDS has evaluated the available data on seropositivity to HIV-1 supplied by research groups in 12 Eastern and Western European countries. The period covered is 1985 and 1986. A significantly elevated incidence of seropositives was observed in both females and males in heterosexual contact with members of high risk groups. In contrast heterosexuals with no such contact had an incidence below 1%. For male homosexuals from Italy, Denmark and Switzerland the trend was no detectable increase in prevalence from 1985 to 1986. Hungary and Poland now have a few per cent seropositive male homosexuals, but no seropositives were found in a group of Polish drug abusers.

Bisexuality↗
PubMed1988

Comparative evaluation of non-radioactive in situ hybridization techniques for pathologic diagnosis of viral infection.

We have compared the efficiency of biotinylated DNA probes and various visualization techniques with 35S-labeled DNA probes in routine paraffin sections from the pathology service; both autopsy and biopsy tissue were investigated. Probes included DNA genomic fragments from cytomegalovirus (CMV), Epstein-Barr-Virus (EBV) and human immunodeficiency virus (HIV). Methods of detection by Pathogene II kit (ENZO) and Blue Gene kit (BRL) with visualization by AEC, NBT/BCIP, Immunogold (Janssen) and autoradiography were used. The study shows most satisfactory results by applying the Blue Gene-NBT/BCIP combination followed by the Immunogold technique. Data obtained by these techniques compare well to those of using radioactive DNA probes and autoradiography.

Acquired Immunodeficiency Syndrome↗
PubMed1987

Persistent active Epstein-Barr virus infection and atypical lymphoproliferation. Report of two cases.

Two cases of what is apparently Epstein-Barr virus (EBV)-associated atypical lymphoproliferation which clinically suggested malignant lymphoma are discussed. Immunohistology revealed polyclonal T- and B-cell proliferation resembling progressive infectious mononucleosis. Giant cell formation and marked plasmacytosis was suggestive of Hodgkin's disease. EBV serology was initially not diagnostic, but during the course of the disease, elevated titers against early antigens and EBV-associated nuclear antigen suggested virus replication. EBV DNA was demonstrated in lymphoid cells of both cases by in situ hybridization. One case progressed to overt monoclonal B-cell malignant lymphoma. Atypical polyclonal lymphoproliferation in persistent active EBV infection may thus be considered a prelymphomatous condition.

Antigens, Viral↗
PubMed1987

Cell membrane fluidity in chronic lymphocytic leukemia (CLL) lymphocytes and its relation to membrane receptor expression.

Cell membrane fluidity (CMF), Ig receptor expression and ecto-ATPase activity were investigated in lymphocytes from patients with chronic lymphocytic leukemia (CLL) and from healthy controls. Significant differences were observed in the parameters of individual CLL cases as compared to normal controls. These findings suggest that malignant transformation of lymphocytes to CLL cells is accompanied by disturbances in cell membrane structure and function, which can be modulated to some extent by exogenous influences on the lipid composition of the cell membrane. The results also suggest, however, that CLL is apparently an inhomogeneous disease with major variations in the investigated parameters among individual cases.

Adenosine Triphosphatases↗
PubMed1986

DNA image cytometry in acquired immune deficiency syndrome (AIDS).

In nine cases with the acquired immune deficiency syndrome (AIDS), including four stage I cases, three stage II cases and two stage III cases, DNA image cytometry was performed on Feulgen-stained lymph node imprint smears. Diploidy was found in three cases, tetraploidy in three cases and octoploidy in two cases. Aneuploid DNA distribution patterns were not seen. The lymphoid cells showed an enormously increased proliferation rate. Two cases in stage I revealed characteristic intranuclear DNA inclusions in lymphoid cells. These results indicate that DNA image cytometry may be useful as an adjunct to surgical pathology in certain cases to assist in the differential diagnosis between AIDS and benign conditions of the lymphoid system as well as between AIDS and malignant lymphomas, which usually have aneuploid DNA patterns.

Acquired Immunodeficiency Syndrome↗
PubMed1985

[New effective therapy of systemic infection with Nocardia asteroides].

A combination of amoxicillin with clavulanic acid (a beta-lactamase inhibitor) and the aminoglycoside amikacin proved effective in the treatment of advanced pulmonary nocardiosis in a 49-year-old man. The result accorded with preceding in-vitro and animal experiments and justifies this combination of drugs in the treatment of nocardiosis, especially if the causative organism is N. farcinica (N. asteroides, biovariety B).

Amikacin↗
PubMed1985

Immunopathology of AIDS (acquired immune deficiency syndrome) observations in 75 patients with pre-AIDS and AIDS.

Sequential changes in the morphology of immunocompetent and other organs, in the accompanying immunocytological and immunovirological measurements are described in 75 patients with lymphadenopathy syndrome and with AIDS. The data presented demonstrate a stepwise development of the disease from a hyperimmunization syndrome to T-cell immune deficiency. Excessive antigenic stimulation by a large number of infectious organisms or by transfusion of blood and blood products account for antigenic overloading and hyperimmunization. Among such infectious organisms are certain viruses which per se interfere with cells of the immune system as for instance Epstein-Barr virus, cytomegalovirus, and HTLV3. Developing immunological incompetence will favor the persistence of these and other infectious organisms enhancing the damage of the immune reactivity and finally allow lethal infections or malignant tumors to occur.

Acquired Immunodeficiency Syndrome↗
PubMed1985

[Somatostatinoma of the liver].

A case report is given of a 45 year old patient, who had a tumor of the liver, pain in the upper abdomen and relapsing fever. Surgery was done and part of the liver was removed. On microscopic examination a somatostatinoma was found. The clinical symptoms of this very rare type of tumor, which belongs to the APUD cell system, is discussed as well as therapeutical implications.

Adenoma, Islet Cell↗
PubMed1984

[Special pathologico-anatomical aspects of AIDS--acquired immune deficiency syndrome].

Data from morphologic and immunologic follow-up studies are presented of ten patients with AIDS. The sequence of morphologic lesions in lymph nodes proceeds from an initial marked hyperplasia of T- and B-zones (follicular hyperplasia with plasmacytosis and diffuse paracortical hyperplasia) to paracortical atrophy with reactive reticulo-histiocytic hyperplasia and to a late irregular B-cell hyperplasia with effacement of lymph node structure. These changes are accompanied immunocytological by a polyclonal increase in B-Lymphocytes while T-helper cells (TH) and T-suppressor cells (TS) initially exhibit a normal 2:1 ratio, an inversion of the TH/TS ratio is observed in advanced stages. Inversion occurs at the time when paracortical atrophy becomes evident in lymph nodes. Pathogenetically, persistent virus infections and a self-sustaining disturbance of immune regulatory mechanisms are discussed.

Acquired Immunodeficiency Syndrome↗
PubMed1984

[Nodular fasciitis of the orbit].

A 46-year-old patient is described who presented with a pea-sized painless tumor in the upper eyelid. Surgical excision and histologic diagnosis revealed a proliferating spindle cell tumor with an inflammatory reaction, which was identified as nodular fasciitis. Differential diagnosis, incidence of such tumors in the orbit and treatment are discussed.

Diagnosis, Differential↗
PubMed1983

A new working formulation of non-Hodgkin's lymphomas. A retrospective study of the new NCI classification proposal in comparison to the Rappaport and Kiel classifications.

Two hundred thirty cases of malignant non-Hodgkin's lymphomas were reclassified in a retrospective study according to the New Working Formulation for Clinical Usage of the NCI as compared to the Rappaport and Kiel classifications. The reproducibility for the individual schemes this study was 81% (Rappaport), 79% (Kiel), and 85% (New Working Formulation). In keeping with the results of the NCI international study, all lymphomas were subdivided into 3 prognostic groups: (1) low-grade malignancy (6.0 years median survival); (2) intermediate-grade malignancy (3.5 years median survival); and (3) high-grade malignancy (1.4 years median survival). The NCI-proposed New Working Formulation for Clinical Usage is thus recommended as practical and unprejudicing classification scheme for general application; however, its usefulness as tool for translating one classification scheme into another appears limited.

Europe↗
PubMed1983

Quantitative light and electron microscopic changes in thymic reticular epithelial cells during Moloney-virus-induced lymphoma development.

This report describes two types of reticular epithelial cell in the thymic cortex of the BALB/c mouse, an immature and a mature form. During early stages of lymphoma development, i.e., 2-6 weeks postinfection (p.i.) with Moloney leukemia virus (M-MuLV), activation of the epithelial cells is observed. Although the percentage of these cells in the total cell population of the thymic cortex remains constant during that time, the number of mature epithelial cells is significantly increased in infected animals. Subsequently, about 6 weeks p.i., the number of immature epithelial cells starts to increase, whereas the number of mature reticular epithelial cells declines and the appearance of the mature epithelial cells changes drastically. The results of light and electron microscopic studies indicate degeneration of the mature reticular epithelial cells at the onset of lymphoma development at a time when the first deficiencies in the immunologic competence of the reticular epithelial cells are apparent.

Animals↗
PubMed1983

Differentiation block of prethymic lymphocytes during Moloney-virus-induced lymphoma development associated with a thymic epithelial defect.

Previous cytokinetic studies in Moloney-virus (M-MuLV)-induced lymphomas of BALB/c mice showed an intrathymic maturation block of prethymic lymphocytes derived from hematopoietic tissues. Thymus-cell cultures during the latent period of lymphoma development showed a proportion of nonlymphoid cells (NLC) from uninfected mice of 0.1% (3 days) to 0.002% (20 days), rising in infected mice to 1-2% after 5 weeks. Concomitantly, thymic epithelial cells exhibit progressive degenerative changes in vivo in infected mice with virus replication and in vitro a marked cellular polymorphism with nuclear atypia becomes overt. Immunofluorescence studies of thymopoietin II and serum thymus factor in epithelial cells indicate a marked decrease of these hormones in the epithelial cells from infected mice. These results suggest a functional defect of virus-infected thymic epithelial cells which causes a progressive accumulation of nondifferentiating T-cell precursors.

Animals↗