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G R Krueger

Publications and source records attributed to G R Krueger.

At least 73 records · Page 4Linked to original sources
PubMed1983

Epstein-Barr virus-related lymphocyte stimulation inhibitor: a possible prognostic tool for undifferentiated nasopharyngeal carcinoma.

It is demonstrated in this study that a serum factor, a lymphocyte stimulation inhibitor (LSI), which inhibits Epstein-Barr virus (EBV)-induced lymphocyte stimulation, is a potentially useful tool in the diagnosis and monitoring of nasopharyngeal carcinoma (NPC). In a study of 25 patients with undifferentiated NPC, 20 healthy controls, and 20 patients with other head and neck tumors, LSI was found only in the NPC patients with active disease. In a more complete study of 8 patients longitudinally followed up for at least 20 months, a comparison of LSI with antibodies to a variety of EBV antigens including viral capsid antigen, early antigen, and nuclear antigen indicated that LSI levels provided a reliable and sensitive indicator of disease activity that should be added to clinical markers currently in use as monitors of disease activity in NPC.

Adult↗
PubMed1982

Sera from patients with undifferentiated nasopharyngeal carcinoma contain a factor which abrogates specific Epstein-Barr virus antigen-induced lymphocyte response.

A unique association of Epstein-Barr virus (EBV) with the undifferentiated nasopharyngeal carcinoma (NPC) is a well acknowledged phenomenon. We report here the detection of a factor present in the sera of NPC patients which inhibits the blastogenic response of lymphocytes from EBV seropositive individuals to EB virions or soluble antigens. This lymphocyte-stimulation inhibitor (LSI) was found to be associated with the IgA fraction of the serum immunoglobulins. No inhibitory activity was detected in the sera and their immunoglobulin fractions from healthy (both EBV-seropositive and seronegative) individuals and patients with other carcinomas of the head and neck region. Interestingly, the IgA-LSI was absent in the sera of NPC patients who were successfully treated and remained in remission, while it was readily detectable in the sera of NPC patients in relapse, LSI-positive IgA fractions did not inhibit mitogenic response of lymphocytes to phytohemagglutinin. Taken together, the data presented suggest that LSI is a specific inhibitor of the response of sensitized lymphocytes to EBV antigens and that it may indeed represent a marker of great clinical significance regarding undifferentiated nasopharyngeal carcinoma, particularly for its prognosis.

Antigens, Viral↗
PubMed1982

Classification of malignant lymphomas of the mouse using morphological, immunological, and cytochemical methods: a working proposal.

We review techniques used to classify malignant lymphomas of the laboratory mouse. Besides an initial morphologic classification according to the Rappaport scheme for human lymphomas, individual tumor types were further subclassified by use of immunocytological T- and B-cell determinations as well as by histochemical procedures. The results, which allow a certain appraisal of the maturation stage of lymphoma cells, are discussed taking as examples six different experimental mouse lymphomas.

Animals↗
PubMed1982

[Lymphoepithelial carcinoma of the oropharynx: incidence and differential di].

From 1960--1980, 452 malignant tumors of the oro- and nasopharynx were observed in the Cologne university ENT-clinic. 313 tumors of the oropharynx and 119 of the nasopharynx could be reclassified using the current classifications of upper respiratory tract tumors (WHO and Cologne modification) and of malignant lymphomas (German Kiel and Rappaport classifications). The analysis showed a different distribution of histological diagnoses in the naso- and oropharynx. Whereas the squamous cell carcinoma dominates with 57% in the oropharynx it is seldom seen (only 13%) in the nasopharynx. The non-keratinizing the undifferentiated carcinomas showed an inverse distribution in both regions. Malignant lymphomas and other types of malignant tumors occur equally frequent in the naso- and in the oropharynx. Only patients with non-keratinizing and undifferentiated carcinomas of the nasopharynx showed elevated antibody titers against Epstein-Barr virus. 7 patients with tumors of the same histological type but localization in the oropharynx had no elevated antibody titers against EBV.

Adult↗
PubMed1981

T- and B-cell determination in various lymphoid tissues of mice during N-nitrosobutylurea (NBU) leukemogenesis.

T- and B-cell distribution in thymus, spleen, and lymph nodes has been investigated during chemical lymphomagenesis. N-nitrosobutylurea (0.04 g/100 ml) in drinking water was administered to 35-day-old male C57BL mice for a period of 60 days. As of week 11, 21 of 22 Nbu mice developed thymic lymphomas of lymphoblastic cell type which showed a general leukemic spread to spleen, lymph nodes, and several extralymphatic organs at week 16. Immunocytologic studies revealed the T-cell origin of these thymic type lymphomas. Alteration of the T-cell system during latency period was represented by reduced thymic weight, cell numbers, as well as absolute and relative T-cell reduction. Absolute T-cell reduction was also observed in the spleen, although the reduction of splenic lymphoid cells was mainly caused by a decrease of B cells. In lymph node subnormal variations of theta-positive cells took place, whereas B-cell distribution was not altered. An impaired T- and B-cell function was suspected from preleukemic changes of lymphoid subpopulations. The results are compared with other murine lymphomas and discussed with regard to a functional hypothesis of lymphomagenesis.

Animals↗
PubMed1981

Histological types of nasopharyngeal carcinoma as compared to EBV serology.

One hundred and thirteen cases of nasopharyngeal carcinoma (NPC) were classified histologically according to the WHO-, French and Cologne systems. The various histological tumor types were then correlated with data on Epstein-Barr virus (EBV) serology (EA, VCA, EBNA, CF, IgA-VCA). The results showed non-keratinizing carcinomas with lymphoid infiltration, and undifferentiated carcinomas to be associated with significantly elevated anti-EBV titers. These two tumor types can be easily grouped together according to the French classification scheme as "undifferentiated carcinoma of the nasopharyngeal type (UCNT)". Such a procedure, which may have therapeutic implication, simplifies the rapid diagnostic screening of NPC patients and also may enhance the reproducibility of histological tumor typing.

Antibodies, Viral↗
PubMed1980

[Sézary syndrome (author's transl)].

Morphological demonstration of the typical Sézary cell in peripheral blood confirmed the diagnosis of Sézary syndrome in a 64-year-old patient with generalized erythrodermia and typical histopathological skin changes. Enzymes and immunocytological membrane characteristics of Sézary cells changed in the course of the disease after cytostatic therapy. Polychemotherapy clinically led to transitory complete regression of skin changes. Reviewing the few published investigations it is found that the morphologically sufficiently defined Sézary cells are apparently not uniform cytochemically and immunocytologically in different patients.

Autopsy↗
PubMed1980

Immunocytological studies in a case of Bruton's disease.

Immunopathological findings in a case with infantile X-linked agamma globulinemia (Bruton's disease) are reported and compared to similar data from two cousins of the patient. Besides a severe decrease in B-lymphocytes in blood and lymph node, residual B-cells showed an almost total defect in Ig surface receptor expression. In addition, there was an unusual lymphocyte population which apparently exhibited E-rosette formation (i.e. T-cell characteristics) and phagocytosis. The data suggest that in Bruton's disease there may be a disturbance of the T-cell system besides the well known B-cell deficiency.

Agammaglobulinemia↗
PubMed1980

Sézary syndrome: immunocytological and cytochemical variability of Sézary cells.

The characteristic large cells in the blood of a patient with Sézary syndrome underwent immunocytological and cytochemical changes during polychemotherapy, which caused transient regression of skin lesions. Tartrate-resistant acid phosphatase was demonstrable in a few cells only after chemotherapy; initially, only 2% T cells and 26% B cells could be demonstrated in the blood by immunocytological methods; after cytostatic therapy, 35% T cells but no B cells were detected.

Acid Phosphatase↗
PubMed1979

[Angioimmunoblastic lymphadenopathy and persistent virus infection? Discussion of immunohistological findings on two cases (author's trsnsl)].

The presence of rubella virus antigen was demonstrated by means of immunohistological methods in two cases of angioimmunoblastic lymphadenopathy. One patient had elevated serum anti rubella titer and myocarditis. These findings support the idea that angioimmunoblastic lymphadenopathy develops as a combined effect of persistent virus infection and partial immune deficiency.

Aged↗
PubMed1979

Distribution of T- and B-cells and of immunoglobulin-producing cells in tumor tissue of patients with nasopharyngeal carcinoma.

Various subtypes of 17 European nasopharyngeal carcinomas are examined by immunocytological and immunohistological methods. The T- and B-Cells in the inflammatory infiltrate are determined quantitatively and correlated with the amount and type of intracellular immunoglobulin. This data is compared with peripheral T-Cell values and the serum anti Epstein-Barr virus (EBV) titers, and finally with the tumor type and stage. Three of the 17 patients had marked local immunoglobulin producing cells, 8 only moderate, and 6 had nearly none. The intracellular immunoglobulin was primarily IgG/kappa, and no significant increase of IgA producing cells was observed as suggested by others. No clearcut correlation was found between local T- and B-Cell infiltrates and the tumor type, stage of disease, or anti EBV titers in the patients' serum. The possible implication of this data is discussed.

Antibodies, Viral↗
PubMed1979

Veno-occlusive disease of the liver after allogeneic bone marrow transplantation: possible association with graft-versus-host disease.

Acute veno-occlusive disease of the liver developed in seven of 29 patients undergoing bone marrow transplantation for treatment of leukemia, aplastic anemia, or disseminated carcinomatosis. All seven died despite successful marrow engraftment. Hepatic failure was the principal cause of death in four and contributory in three. The veno-occlusive disease did not relate to the nature of the pretransplant immunosuppressive regimen, since it occurred in patients receiving irradiation alone, chemotherapy alone, or both. Twenty-two of the patients were autopsied. Among these, the lesion was found in seven of 11 in whom a graft-versus-host reaction developed but in none of the 11 without such a reaction who had received similar pretransplant immunosuppression (P less than 0.025). Hence, acute veno-occlusive disease of the liver appears to be a complication of allogeneic bone marrow transplantation related to the development of a graft-versus-host reaction.

Acute Disease↗
PubMed1979

Classification of animal lymphomas: the implications of applying Rappaport's classification for human lymphomas to experimental tumors.

One hundred and seventy animal lymphomas (species ranging from molluses to monkeys) were reclassified histologically according to the modified Rappaport classification for human lymphomas. The results were correlated with the etiology of the lymphomas, their clinical course, and in selected cases with their immunological type. The study stresses the value of such a procedure for comparative reasons, allowing a more adequate selection of animal models for human lymphomas.

Animals↗
PubMed1978

Sequential changes of T- and B-cells, virus antigen expression and primary histologic tumor diagnosis in virus-induced lymphomagenesis of mice.

T- and B-cell counts, estimation of Ig receptor fluidity, and expression of virus-coded antigens were correlated with histological findings during the development of virus-induced mouse lymphoma. Tested were BALB/c mice after infection with the strongly oncogenic Moloney leukemia virus (MLV), the moderately oncogenic (in BALB/c mice) Gross passage A virus (GLV-A), and the essentially non-oncogenic Gross 3T3 tissue culture virus (GLV-T). Methods included immunofluorescence microscopy with antisera against T-cells, B-cells and MLV intact virus, routine histology, and electron microscopy. Following time sequence of changes was observed in mice with oncogenic MLV- and GLV-A infection but not in GLV-T infection: Significant decrease of Ig receptor fluidity and expression of virus antigen were observed already at the initial investigation, i.e. 2 weeks post virus infection. This was followed by significant decreases in percent T-cells 5--8 weeks later, accompanied by histologic atrophy of the thymus and of thymus-dependent regions of lymphatic tissues. Another 2--8 weeks after the decrease in percent T-cells occurred, the first lymphomatous foci became obvious in the thymus. Clinically overt and generalized lymphoma was diagnosed at 20--30 weeks post virus infection. Ultrastructurally, some changes in the arrangement and quantity of cytoplasmic microfilaments were noted in proliferating lymphoblasts and in lymphoma cells. It is concluded, that the described changes were related to the oncogenic potential of mouse C-type RNA viruses and not just to virus infection per se.

AKR murine leukemia virus↗
PubMed1978

Combined immune deficiency syndromes with primary T-cell defect and partial B-cell reactive hyperactivity. Immunological and morphological analysis of two unusual cases.

Two cases of combined immunodeficiency with lymphopenia, thymic dysplasia, and defective immunoglobulin production are reported. Both show selective hypo-gammaglobulinemia (IgG and IgA respectively) and selective hyper-gammaglobulinemia (both IgE, IgA, and IgM respectively). The cases are classified, by correlation of clinical and histopathological data as a variant of Fireman's disease.

B-Lymphocytes↗