Biomedical subjects

G Sani

Publications and source records attributed to G Sani.

At least 55 records · Page 3Linked to original sources

PubMed1993

[The role of prostaglandins in fertility. Experimental research].

Prevention of postoperative adhesion is an issue that continues to elude the abdominal and reproductive surgeon. Adhesions seem to be a result of an inflammatory process and it is well known that prostaglandins play an important role in such an event. In an attempt to improve the results of microsurgery, we have tried in this study to examine the effect of local intraperitoneal application of prostaglandins (PGE2 and PGF2 alpha) on adhesion formation in the rat after traumatizing to the uterine horn. Prostaglandins applied locally were found to increase intraperitoneal adhesion formation at the injured sites, in comparison with controls. Also, we have reported on histological examination an increased accumulation of inflammatory cells in traumatized areas. However, we didn't observe a reduced fertility in rats treated with prostaglandins because these substances induce follicular rupture by activation of proteolytic enzyme located in the follicular wall. We conclude that prostaglandins play an important role in the process of adhesion formation. Using antiprostaglandins agents could improve the outcomes of reproductive surgery.

Animals↗

PubMed1992

[Microsurgery and laser surgery in conservative operations on the ovary. Experimental research].

In this study we compared two different conservative surgery techniques performed on 12 ovaries of female rabbits: microsurgery and CO2 Laser surgery. After the surgical procedure all the animals were investigated by a Laparotomy to evaluate the post-operative adhesion formation. Histological examinations were performed on 6 ovaries, to evaluate the possible damage to the ovarian parenchyma. We did not find significant differences between the two methods employed, particularly for the adherence formation and the parenchymal thermic damages: no post-operative adhesions were detected respectively in 3 ovaries operated on by microsurgery and 5 by laser surgery; slight adhesions were present in 2 ovaries treated with microsurgery and in 3 with laser surgery; 3 ovaries treated with microsurgery and 2 with laser surgery showed moderate adhesions. Only 2 ovaries treated with microsurgery presented severe adhesions.

Animals↗

PubMed1992

[Tubo-ovarian transplantation in the treatment of sterility. Experimental research].

Microsurgical transposition of fallopian tube and ovary has the potential of being an efficient therapeutic treatment in patients with tubal sterility. The Authors present their experience of microsurgical adnexal transplantation in rabbit by two different techniques: the first procedure by microvascular anastomosis of the ovarian vessels, the second one without vascular pedicle. Function is evaluated at various time after grafting by: exploratory laparotomy on day 30 to establish whether circulation to the grafts was still maintained; macroscopic and microscopic examination of ovaries and fallopian tubes. The microvascular techniques prove highly reliable in terms of immediate vascular patency rate but it is disappointing that 50% of the autografts has failed with blocked vessels by day 30. Perhaps this is due to the difficult techniques in anastomosing the ovarian vessels of small caliber. In spite of these outcomes the vascularized autografts were viable and functional after transplantation in contrast with the non-vascularized tubo-ovarian grafts which all failed. This experience encourages to believe that the microsurgical technique could be employed for homograft transplantation in woman with extensive ovarian and tubal damages.

Anastomosis, Surgical↗

PubMed1990

Increased placental production of leukotriene B4 in gestational hypertension.

Production of some lipoxygenase and cyclooxygenase derivatives of arachidonic acid was measured in placental tissue obtained from women with gestational hypertension and with normal pregnancies. The levels of leukotriene B4 were about five times higher in placentas from hypertensive women and also raised thromboxane A2 and reduced prostaglandin E2 levels were observed. Prostacyclin production was lowered only in women with more severe hypertension, in association with the highest measured levels of leukotriene B4 and thromboxane A2. It is suggested that increased placental levels of leukotriene B4 and thromboxane A2 appear already in mild gestational hypertension, while depression of prostacyclin may occur only at more severe stages of gestational hypertensive disease.

Adult↗

PubMed1990

[Post-infarction pseudoaneurysm of the left ventricle as a cause of heart failure. Presentation of a surgically treated case].

Pseudoaneurysm of the left ventricle is an unusual complication of myocardial infarction. Surgical resection is justified by the high risk of rupture. Patients may be asymptomatic, more often a clinical state of chronic heart failure is present. We report on a patient who successfully underwent urgent resection of a large pseudoaneurysm which was the cause of acute heart failure.

Emergencies↗

PubMed1990

[Effects of ovarian and peritoneal endometriosis on fertility. Experimental study].

Fertility after ovarian and peritoneal endometriosis experimentally induced has been studied. The fertility rate in the animals with ovarian endometriosis was decreased of 40% and the same reduction was found in the animals with peritoneal endometriosis. Not only the direct effect from adhesion formation, but also changes in the peritoneal fluid or its constituents are proposed as causes of endometriosis-associated infertility.

Animals↗

PubMed1989

[Ultrastructural changes in the saphenous vein removed during aorto-coronary bypass].

In order to observe ultrastructural changes of the saphenous vein before implantation during coronary artery by-pass surgery, 58 fragments from a series of 29 consecutive patients, were studied by means of scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Two fragments were taken: the first just before the distal anastomosis; the second just before the last anastomosis on the aorta. The vein graft was kept in a finger solution of 10% nitroglycerin. Forced dilatation was avoided. The EM showed areas of early loss of the endothelial layer, not time-related, deposits of fibrin and blood corpuscular elements with muscular contraction in 75% of the examined grafts. From our experience and according to the literature, loss of the endothelial layer, spasm and early platelet and fibrin aggregation, are constant features of the explanted saphenous vein. In clinical practice, we emphasize, in addition to the "no touch technique", the early use of antiplatelet drugs during coronary artery by-pass coronary surgery.

Coronary Artery Bypass↗

PubMed1989

[Morpho-functional study of the rabbit ovary after cauterization].

The authors investigated the lower incidence of post-operative adhesion formation after cauterization in comparison with microsurgical wedge resection in rabbit ovaries. Microscopic examination of cauterized ovaries did not show extensive necrosis in the depth of ovarian tissue. Electrocautery seems to be an effective alternative to ovarian wedge resection.

Animals↗

PubMed1989

[Use of prostheses made of ePTFE in anastomoses of the rat uterine horn].

ePTFE prostheses were used in the microsurgical anastomosis of the rat uterine horn. Handling characteristics of this synthetic material during the performance of anastomosis were tested. Patency rates, infections and local tissue reaction were evaluated after thirty and sixty days. Connective tissue infiltration and incomplete endothelialization by normal endometrial elements were observed on the luminal surface of the prosthesis.

Anastomosis, Surgical↗

PubMed1988