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Biomedical subjects

G Tedde

Publications and source records attributed to G Tedde.

At least 37 records · Page 2Linked to original sources
PubMed1978

Mitotic index of the Langhans' cells in the normal human placenta from the early stages of pregnancy to the term.

The mitotic index of the Langhans' cells of the normal human placenta has been evaluated from the 6th week of pregnancy until the term. The index is very high from the 6th to the 10th week, and afterwards it decreases quickly. However, some dividing cells are present also in placentas of 30-32 weeks. The high number of mitotic cells is considered to be related to the increase of the syncytial layer more than to the HCG production. This latter would be independent of the proliferative activity of the Langhans' cells and is probably affected by some humoral factors.

Chorionic Villi↗
PubMedDate not supplied

An experimental study of the effect of ischemia on testicular structure in the rat. Efficacy of intravenous inosine.

The damages caused by experimental testicular ischemia lasting 60 minutes have been examined in the rat. The histologic alterations of the germinal epithelium and of the tunica propria are studied, using semi-thin sections stained with 1% toluidine blue. The results show that a testicular ischemia of 60 minutes causes damages to the testicular structures. These damages cannot be prevented or avoided by either surface cooling or cold perfusion; however, intravenous inosine prior to ischemia has a protective effect.

Animals↗
PubMedDate not supplied

On a particular method of isolation of the human placenta trophoblastic cells.

With the purpose of demonstrating the presence of different types of cells in the syncytial and cytotrophoblast of the human placenta, a new technique of cell isolation was performed by utilizing a light enzymatic digestion and a separation on density gradients. Normal human placentas of the first trimester of pregnancy have been studied. After an accurate and light washing in saline and anticoagulant substances, whole villi have been incubated in a trypsin solution for various periods of time at 40 degrees C in a thermostatic stirrer. Detached cells have been collected, rinsed and separated by means of different density gradients of Percoll (d = 1.038 and 1.080). Three cellular layers have been collected and processed for the studies at light and electron microscopy. The first layer was mostly composed by multinucleated elements with a morphological pattern closely related to the histological characteristics of the syncytiotrophoblast; the second fraction was composed by mononucleated elements with the structural findings of the Langhans' cells; the third layer was represented almost exclusively by blood cells. The obtained results demonstrated the high utility and accuracy of the suggested method of cell isolation.

Cell Fusion↗
PubMedDate not supplied

Mechanisms of regulation of the capillary bed in the human chorionic villi.

The purpose of the present researches was to confirm the hypothesis that in the human placenta the microcirculation is subjected to humoral control, because of the absence of innervation in the chorionic villi and the ability of the capillaries to contract themselves. Chorionic villi were incubated in presence of vasoactive substances, Histamine, Serotonin, Bradykinin, Dopamine, Enkephalin Prostaglandins. After incubation, the specimens were fixed and embedded in resin. Semithin sections were submitted to a computerized analysis for the evaluation of the ratio between surface of capillaries and surface of the villus. The results confirmed that in the control of capillaries in the human placenta most of the tested substances show a specific action, dose- or time-dependent.

Capillaries↗