H Chua — Page 3 | PubMed Health

PubMed1996

Filamentous growth in activated sludge.

Bulking and foaming in activated sludge have been associated with filamentous overgrowth. Filamentous Nocardia amarae and nonfilamentous Pseudomonas auruginosa were cultured using fatty acids (C2-C24) as the sole carbon. N. amarae could utilize all acids tested for growth, whereas P. auruginosa hardly grew on acids with 12 or more carbons. Maximum specific growth rate and saturation constant of N. amarae on C24, at 0.048 h-1 and 1.520 g COD/L, respectively, were much lower than that of P. auruginosa, showing that N. amarae had a relatively stronger affinity for long-chain fatty acids. N. amarae was competitive in activated sludge processes that receive sewage containing a high proportion of long-chain fatty acids, oils, and fats.

Carbon↗

PubMed1992

Isolation of 1001 new markers from human chromosome 11, excluding the region of 11p13-p15.5, and their sublocalization by a new series of radiation-reduced somatic cell hybrids.

The determination of the physical map of human chromosome 11 will require more clones than are currently available. We have isolated an additional 1001 new markers in a bacteriophage vector from a somatic cell hybrid cell line that contains most of chromosome 11, except the middle of the short arm. These markers were localized to five different regions, 11p15-pter, 11p12-cen, 11q11-q14, 11q14-q23, and 11q23-qter, by a panel of previously characterized somatic cell hybrids. The region 11q11-14 harbors genes that have been shown to be important in breast cancer, B-cell lymphomas, centrocytic lymphomas, asthma, and multiple endocrine neoplasia, type 1 (MEN1). To determine the positions of the recombinant clones located there, we developed a new series of radiation-reduced somatic cell hybrids. These hybrids, together with those previously characterized, allowed us to map the 11q11-q14 markers into 11 separate segregation groups.

Asthma↗