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H Festenstein

Publications and source records attributed to H Festenstein.

At least 37 records · Page 2Linked to original sources
PubMed1978

Cellular typing.

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Epitopes↗
PubMed1978

Non-major histocompatibility system immunogenetic influences in rat renal allograft survival.

H-1-incompatible rat renal allograft survival rates are strongly influenced by the non-major histocompatibility system (MHS) genes. The same H-1 (MHS) incompatibility tested on four different non-MHS backgrounds gave widely differing results ranging from 11 to 60% fractional survival between the best and the poorest (P less than 0.01) at 105 days. These results can best be explained if non-H-1 and intra-MHS amplifiers and/or suppressors are invoked.

Animals↗
PubMed1978

The role of H-2 and H-2-like determinants in syngeneic and allogeneic cytostasis.

Specific sensitization against H-2 determinants was affected by immunizing allogeneic mice with spleen and lymph node cells in H-2 congenic combinations. Lymph nodes from the sensitized and non-sensitized mice were respectively cultured together with H-2 syngeneic tumour cell lines. The growth and viability of the tumour cells was subsequently measured by the amount of radiothymidine incorporation. If the tumour cells incorporated less isotope when cultured with the immune cells than with the normal cells this was termed 'cytostasis'. To identify the H-2 genes controlling the sensitization phase in the cytostasis assay, we studied the effect on different transplantable tumour target cells of lymphoid cells from mice sensitized against different congenic spleen cells. The results suggest that the cytostasis assay can can measure an in vitro specific response to H-2-incompatible sensitizing antigens, and that I--B incompatibility, together with K and/or D, is essential to produce effectors. Furthermore, H-2 allogeneic sensitization could induce cytostasis even against tumour cells syngeneic for the H-2 halotype of the responder strain. The implications of these findings are discussed.

Animals↗
PubMed1978

The role of antibody in the inhibition of the growth of Meth.A tumour in syngeneic experiments in vivo and in vitro.

An in vitro technique for detecting anti-tumour responses was studied and shown to involve non- T cells. Further examination of the effector mechanism revealed that tumour inhibition was antibody mediated, probably through complement dependent lysis; ADCC was considered unlikely. The amount of antibody involved was small, as shown by indirect immunofluorescence labelling of tumour cells, but was nevertheless effective in vitro and in causing regression of tumours in vivo. These findings may have important implications for the manipulation of the host responses to tumours.

Animals↗
PubMed1978

Cytostasis against self-antigens.

Effector cells from mice of the C57Bl/10 congenic series, alloimmunized against K end- or D end-identical I region incompatible lymphocytes, were cytostatic for tumour and macrophage targets bearing syngeneic K-end and D-end antigens. If incompatibility during immunization was restricted to the I-B subregion only, cytostasis was directed against K end syngeneic antigens; further incompatibility in the I region was required to obtain cytostasis against D-end self-antigens. Cytostasis against self-antigens was also obtained after immunization with syngeneic tumour cells; in one instance this was directed against syngeneic K-end antigens only. The implications of these findings in relation to neoplastic change and autoimmune damage are discussed.

Animals↗
PubMed1977

Generation of suppressor cells in mice immunized with M locus-incompatible lymphocytes.

Alloimmunization of mice with M locus-incompatible lymphocytes resulted in the generation of suppressor cells in the immunized host. Lymph node cells from such alloimmunized mice suppressed the in vitro cytotoxic response of normal cells to H-2 alloantigens. The suppression generated was greater than could be accounted for by dilution of the prekiller cell population with cells possibly devoid of cytotoxic potential from M locus preimmunized mice. Using M locus pseudocongenic mice, the suppressive effect was shown to be largely attributable to M locus determinants; restimulation of suppressor cells in culture with the specific M locus was required for suppression of effector cell generation. The in vivo effect of suppressor cells was tested in a graft-versus-host reaction; injection of M locus preimmunized cells into footpads of F1 hybrid mice suppressed the popliteal lymph node enlargement compared with lymph node size after injection of control preimmunized cells. Although the suppressive effect is mainly attributable to M locus determinants, incompatibility for the DBA/2 antigen may add to the suppression. The study of inhibitory effects on T cell cytotoxicity because of serologically undetectable lymphocyte-activating determinants (Mls) could lead to the better understanding of suppressive mechanisms which may allow the growth of syngeneic tumours.

Animals↗
PubMed1977

Selection of haploid spermatozoa and its application to HLA-D typing.

The expression of HLA-A, -B, and -D determinants on the surface of spermatozoa is haploid. Using cytotoxicity and appropriate HLA antisera and complement, we were able to select haploid spermatozoa. The surviving sperms behaved similarly to homozygous lymphoid cells when cultured in mixed sperm lymphocyte culture (SLC). This method is being applied to define new HLA-D specificities, to improve typing and matching for bone marrow transplantation and kidney transplantation and to study their association with various diseases.

Complement System Proteins↗
PubMed1977

Emergence of foreign H-2-like cytotoxicity and transplantation targets on vaccinia and Moloney virus-infected Meth. A tumour cells.

Using cytotoxic effector cells of different anti-H-2 specificities, new cell-mediated lympholysis targets, normally undetected on Meth. A tumour cells, were shown after passage with vaccinia or Moloney virus. The H-2d CML targets on Meth. A cells recognized by B10.BR anti-B10.D2 effector cells were presented only after simultaneous vaccinia virus passage, while passage with Moloney virus caused the emergence of H-2b targets. Small but significant killing of vaccinia virus-passaged Meth.A was also obtained by anti-H-2k effector cells. These results are discussed in relation to in vivo experiments: retardation of tumour growth was noted in mice which had received several injections of vaccinia or Moloney virus, showing that the new CML targets were probably acting as transplantation targets.

Animals↗
PubMed1977

Studies on H-2 specificities on mouse tumour cells by a new microradioassay.

Seven mouse tumour cell lines of different aetiology and origin were tested for the expression of surface alloantigens using twenty-four well defined H-2 alloantisera and anti-Thy 1.2. We used a new radioassay that involves antibody-complement treatment of the tumour target cells followed by postlabeling the surviving tumour cells with 14C-thymidine. With a relatively high frequency the anti-H-2 sera were reacting differently with the tumour cells than with respective syngeneic lymphoid cells. Thirty-six anomalous reactions out of 129 investigated were detected. Absorbtion experiments performed with H-2 antigen positive or negative lymphoid cells revealed a striking similarity between these extra-specificities and H-2 specificities of foreign haplotypes. The results are discussed in relation to the biological importance of the extra-specificities, both with regard to origin (derepression) and function (transplantation antigen properties).

Animals↗