Biomedical subjects
Publications and source records attributed to H G Scheil.
A subtyping of the haptoglobins of thirteen small Swiss populations, who live in mountainous regions of the Alps, and four larger Swiss populations has been undertaken. The impact of population and sample size on allele frequencies is discussed.
In an isolate at the Lower Rhine region (North Rhine-Westphalia, Germany) 28 haemogenetic systems have been studied. The immigrants had come mostly from the eastern Hunsrück about 250 years ago. The results have been compared with data from the Lower Rhine region, from Düsseldorf and from eastern Hunsrück (the main origin area of the "Pfälzer"). The cluster analysis shows relatively small genetic distances between these three samples; the population of the isolate is separated by distinct higher genetic distances. The results are discussed considering various population genetic factors.
A population study was carried out on Caucasians from Düsseldorf using the short tandem repeat (STR) system HumVWA (von Willebrand factor; locus 12p12-12pter; intron A). After amplification, electrophoresis and silver staining 9 alleles could be detected in the sample of 304 unrelated individuals. No deviations from the Hardy-Weinberg equilibrium could be observed. The results were compared with other population studies.
The Rhesus haplotype frequencies and their regional distribution have been studied in the administrative area of Düsseldorf, North Rhine-Westphalia. The area studied is characterised by a heterogeneous distribution of the haplotypes without clinical trends and by missing Hardy-Weinberg conditions.
Hypervariable loci within the human genome are useful tools in several disciplines: for example in forensic medicine (paternity testing and forensic identification). One of these genetical markers is located at chromosome 1 and is called D1S80 (MCT118). The genotype distribution and the allele frequency of the VNTR locus D1S80 have been studied in a population of 378 unrelated Germans living at Düsseldorf. The determination of genotypes has been carried out by using the polymerase chain reaction and subsequent analysis of the amplified products by polyacrylamide electrophoresis followed by silver staining. The data demonstrate that the locus is highly polymorphic with an observed heterozygosity of 75.66%. The frequency distribution found does not meet Hardy-Weinberg expectations. We think that this is not astonishing because we found only 80 of the possible 231 phenotypes (alleles 16-34, 36-37, without anodal and cathodal variants). So before using the D1S80 data in forensic analyses and paternity tests a larger data base has to be established. The data of the Düsseldorf sample are compared with data of studies on other populations.
A population study was carried out on 302 ethnic Germans from Düsseldorf and 273 ethnic Germans living in Northrhine Westphalia using the short tandem repeat (STR) system HumTH01 (TC11). Seven different alleles were detected. No deviations from the Hardy-Weinberg equilibrium could be observed. The results were compared with other population studies. The pooled sample from Germany can be used as database for forensic purposes.
A population study was carried out on 301 ethnic Germans from Düsseldorf (Northrhine Westphalia) using the short tandem repeat (STR) system HumF13B (Coagulation Factor XIIIB). Five different alleles were detected. No deviations from the Hardy-Weinberg equilibrium could be observed. In spite of the successfulness of the system only a few population data exist. A pooled sample from Northrhine Westphalia has to be used as preliminary database for forensic and anthropological purposes.
The Rhesus haplotype frequencies and their regional distribution have been studied in the administrative area of Cologne, North Rhine-Westphalia. The area studied is characterised by a heterogeneous distribution of the haplotypes and by missing Hardy-Weinberg conditions. There are higher CDe- and lower cde-haplotype frequencies in the south-western part. The distribution of the very rare cDue haplotype could be correlated with higher B frequencies in the administrative areas of Cologne and Düsseldorf.
This paper reports PCR-based genotype distribution and allele frequencies of the locus HumFES/FPS (human c-fes/fps proto oncogene). For this study 300 unrelated Caucasoids of German ancestry living in Düsseldorf were analyzed. Seven of the eight alleles previously described and fourteen of the corresponding thirty-six diploid genotypes were detected. The proportions of heterozygotes and homozygotes observed and expected and the parameters of forensic interest, e.g. the mean paternity exclusion chance, were calculated. No deviations from the Hardy-Weinberg equilibrium were observed. A preliminary German data base (3,238 individuals) was created by using the weighted arithmetical mean which included data from Düsseldorf.
Typing of 23 blood groups, serum protein and red cell enzyme polymorphism was performed on two samples from Baden-Württemberg, Germany. One random sample comes from the city of Ulm and its surroundings and the other from a small isolate located in Upper Swabia. While the Ulm sample displayed allele and haplotype frequencies similar to those of other German populations, the sample from the isolate turned out to be different.
The usefulness of PCR systems (D1S80, D17S30, ApoB, TC11, VWA, and SE33) is discussed. The statistical evaluation shows that these systems--together with other systems--are well suited for zygosity testing.
This paper reports on the allele and genotype frequencies for five polymerase chain reaction-based loci determined in a sample (n = 295) from Düsseldorf (Germany). The loci are Low-Density-Lipoprotein Receptor, Glycophorin A, Hemoglobin gamma G, D7S8 and Group-Specific Component. Typing was carried out by using the AmpliType PM PCR amplification and typing kit. The loci were tested for possible divergences from Hardy-Weinberg expectations (HWE). The gene frequencies found can be used in forensic analyses and paternity tests. The data from Düsseldorf were compared with six other studies having been published up today.
The AB0 allele frequencies and their regional distribution have been studied in the administrative area of Köln, North Rhine-Westphalia. The B allele shows relatively high frequencies ( > 0.0800) on both sides of the river Rhein, in the eastern parts of the area and--separated from the Köln region by a region of lower B frequencies--in the Aachen region. The possible causes for the different B frequencies are discussed.
In a sample of 265 individuals from the eastern Hunsrück (Rheinland-Pfalz; Kastellaun, Rheinböllen, Simmern region) 27 hemogenetic polymorphisms have been studied. There were significant differences in phenotype frequencies between the eastern Hunsrück and samples from Rheinland-Pfalz and Hessen in the P1-, GPT- and ACP-system, resp.; the differences are discussed.
The distribution of ABO and Rhesus blood groups in the populations of Cyprus have been studied in 47,759 individuals (about 8% of the Greek-Cypriot population). The data were classified according to the administrative areas of Cyprus and into further 41 smaller units.
The AB0 allele frequencies and their regional distribution have been studied in the administrative area of Düsseldorf, North Rhine-Westphalia. The o allele shows relatively low and the B allele relatively high frequencies in the eastern and south-eastern parts of the region. The high B frequencies are interpreted as a consequence of the immigration from north-eastern Germany and Poland at the end of the 19th and the beginning of the 20th century (labourers) and after 1945.
The hemoglobin of the aardwolf (Proteles cristatus) contains only one component. In this paper, we are presenting its primary structure. The globin chains were separated by high-performance liquid chromatography and the sequences determined by automatic liquid and gas-phase Edman degradation of the chains and their tryptic peptides. The alpha- as well as the beta-chains show 20 exchanges compared with the corresponding human chains. The difference to the masked palm civet (Paguma larvata) and the spotted hyaena (Crocuta crocuta) is marked by 16 and 4 replacements in the alpha-chains and by 10 and 1 in the beta-chains, thus supporting the hyaenid character of the aardwolf. The exchanges at contact positions are shared by other carnivoran hemoglobins.
In a sample of 262 individuals from the Lower Rhine region (Kleve, Goch, Kalkar: Germany) 27 hemogenetic polymorphisms have been studied. There were no significant differences in phenotype frequencies between the Lower Rhine and the Düsseldorf region.