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Biomedical subjects

H H Fudenberg

Publications and source records attributed to H H Fudenberg.

At least 181 records · Page 10Linked to original sources
PubMed1980

Radioimmunoassay for the P2 protein of bovine peripheral nerve myelin.

The P2 protein of bovine nerve root myelin was radiolabeled with 125I in homogeneous solution by the chloramine-T method and purified by gel filtration on Sephadex G-75 to obtain monomeric 125I-P2. Antigen-antibody complexes were isolated by the silica gel, double antibody, or polyethylene glycol techniques by using rabbit antibody to P2. As little as 1 ng/ml P2 could be detected. The RIA was used to measure the P2 content in nerve tissue and isolated myelin. The presence of P2 in spinal cord as well as in the peripheral nervous system was confirmed. Peptides isolated by CNBr digestion of the P2 protein were tested in the RIA. CN-1, comprising 80 to 90 residues from the interior of the molecule displayed complete immunologic cross-reactivity with intact P2. Neither CN-2, representing 18 amino acids from the COOH terminal, nor CN-3, representing 20 amino acids from the NH2 terminal, showed cross-reactivity. Since the major determinant for experimental allergic neuritis in the rabbit is located in peptide CN-2, our present data suggest that the major neuritogen and the major determinant(s) for humoral antibody response in this species may be at different locations within the P2 molecule.

Animals↗
PubMed1980

Binding of immunoglobulin classes to subpopulations of human red blood cells separated by density-gradient centrifugation.

Human erythrocytes (RBC) from whole blood were separated according to their specific densities by centrifugation on a polyvinyl-pyrrolidine-coated colloidal silica matrix (Percoll) into four major subpopulations. By indirect immunofluorescence assay, the most dense RBC subpopulation, with specific density greater than 1.110 g/ml (3%-5% of total RBC), was positive for membrane-bound immunoglobulin; the remaining, less dense subpopulations were negative. IgG was present on 85%-95%, IgM on 28%-32%, and IgA on 15%-20% of the RBC in the most dense population. When these immunoglobulins were eluted, radiolabeled, and used in binding studies with autologous RBC fractions subjected to thermal and/or enzymatic treatment, they reacted specifically with the less dense RBC subpopulations. These results suggest that previously cryptic antigens were revealed by the activity of neuraminidase on the plasma membranes of the treated RBC.

Binding Sites, Antibody↗
PubMed1980

Experimental model of chronic serum sickness. Relationships between proteinuria, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and glomerular and extraglomerular renal pathology.

The evolution of proteinuria in rabbits with experimentally induced chronic serum sickness was followed up longitudinally by analytical and quantitative assays. The results suggest that sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a more sensitive index of kidney malfunction than are total-protein assays or the quantitation of albumin and lysozyme. In some rabbits that showed abnormal proteinuria by SDS-PAGE, no histologic evidence of pathologic damage or of deposition of immune complexes in the kidneys was found. This suggests that SDS-PAGE may detect functional alterations at early stages of kidney damage when the lesions are either undetectable or reversible. In one rabbit that was killed after normalization of the proteinuria, immunofluorescence tests indicated deposition of C3, IgG, and fibrinogen, but there was no histologic evidence of kidney damage.

Animals↗
PubMed1980

Antigenic cross-reactivity of sperm and T lymphocytes.

Since seminal components can mediate immunosuppression in vitro, it is possible that some antigen(s) may be common to both the reproductive and immunologic systems. In a group of 70 couples with unexplained infertility, 50 "autoimmune" males and 40 "isoimmune" females had lower than normal percentages of total T cells (mean values +/- standard error of the mean 63 +/- 2% and 60 +/- 2% for immune males and females, respectively, versus 77 +/- 2% and 78 +/- 5% for 50 normal males and females, respectively; P < 0.001). Sheep red blood cell (SRBC) rosetting of lymphocytes was significantly reduced when SRBC were preincubated with sperm extracts (61 +/- 4% versus 9 +/- 2%; P < 0.001) but not when SRBC were incubated with normal serum or when lymphocytes were preincubated with sperm extracts. Antisperm antibody titers in the patients' sera (48 +/- 13) were correlated with their antithymocyte antibody titers (18 +/- 3) (P < 0.01). Moreover, antithymocyte antiserum (titer 1024) cross-reacted with sperm extract (titer 128), and vice versa. This cross-reactivity was significantly reduced by absorption of the sera with sperm cells (P < 0.001), thymocytes (P < 0.001), or white blood cells (P < 0.005). Absorption of autoimmune sperm extracts and seminal plasmas with thymocytes or sperm cells reduced the Coombs' titers, especially immunglobulin G (P < 0.01) and immunoglobulin A (P < 0.025). Similar results were obtained in passive hemagglutiation, immunofluorescence, and cytotoxicity assays. We conclude that sperm and T cells share a common antigen(s).

Absorption↗
PubMed1980

Measurements of extracellular immunoglobulins as an index of B cell function.

We have developed a method for measuring the activation or suppression of B cells in culture by determination of immunoglobulins released into the extracellular fluid, using a conventional, nonisotopic assay. To avoid the interference of human serum proteins in the assays, cultures were established in medium supplemented with fetal calf serum. This caused some B cell activation, as reflected by the release of small amounts of immunoglobulins (1.5 to 0.7 microgram/ml IgG, 0.8 to 1.4 microgram/ml IgA, 0.6 to 0.7 microgram/ml IgM on day 7), but pokeweed mitogen stimulation resulted in the release of substantially larger amounts of immunoglobulins (8.2 to 4.6 microgram/ml IgG, 3.8 to 2.9 microgram/ml IgA, 8.0 to 4.0 microgram/ml IgM on day 7). Stimulation with Staphylococcus aureus (Cowan I) also resulted in release of immunoglobulins, with somewhat higher levels of IgM, and the release was inhibited by a suppressive substance obtained from Streptococcus intermedius. Time-course curves for immunoglobulin production by cultures stimulated with pokeweed mitogen and S. aureus showed interesting individual variations, but in general the levels began to rise consistently by day 4 or 5 and maximum levels were reached by day 7. These studies show that determination of extracellular immunoglobulins can be used as an index of B cells activation in routine studies.

B-Lymphocytes↗
PubMed1979

Association between immunoglobulin allotypes and immune responses to Haemophilus influenzae and Meningococcus polysaccharides.

Serum samples were collected from 20 healthy White and 33 Black infants before and after immunisation with three doses of diphtheria-pertussis-tetanus vaccine and with one dose of Haemophilus influenzae type b polyribose phosphate vaccine and meningococcal group A and group C polysaccharide vaccines. Antibodies to these immunogens were measured and sera were allotyped for several Gm, A2m, and Km antigens. A highly significant association was found between the Km(1) allotype and the immune responses (difference between post-immunisation and pre-immunisation antibody levels) to H. influenzae and meningococcus C polysaccharides in the White children.

Antibodies, Bacterial↗
PubMed1979

Role of adherent T cells and of B cells in the immune response to purified protein derivative of tuberculin.

The role of monocytes, B cells, and adherent and nonadherent T cells in the response of purified protein derivative of tuberculin (PPD) as measured by [3H]thymidine uptake in vitro has been explored. The response to PPD was found to be highly dependent on monocytes, to approximately the same extent as previously found for the responses to concanavalin A (Con A) and phytohemagglutinin. The response to PPD was also found to be highly dependent on a population of adherent T cells different from the adherent T cell population involved in the response to Con A. In the case of PPD, the effect was additive, as opposed to the potentiating effect seen for Con A. Further, the adherent T cells involved in the response to PPD were much more sensitive to hypotonic shock than those in the response to Con A. B cells were also found to be important in the response to PPD, although only to a slight extent.

B-Lymphocytes↗
PubMed1979

Autoantibodies in healthy subjects of different age groups.

Autoantibody determinations in 1284 healthy Caucasian subjects of various age groups were made by indirect immunofluorescence for anti-nuclear, anti-gastric, and anti-thyroglobulin antibodies. A sex-dependent relationship between age and prevalence of anti-gastric and anti-thyroglobulin antibodies was found. No association was found between age and anti-nuclear antibodies.

Adult↗