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Biomedical subjects

H Hübner

Publications and source records attributed to H Hübner.

At least 19 recordsLinked to original sources
PubMed1992

Effects of calcipotriol (MC 903) and calcitriol after topical application on the skin of hairless rats. Much lower effect of calcipotriol on systemic calcium homeostasis.

Topical application of calcitriol or its analogs is a new approach for treating psoriasis, but may be limited by systemic calcitropic effects. Calcipotriol (MC 903) is a novel calcitriol analog with low calcitropic potency after systemic application. To compare the topical potency of calcitriol and calcipotriol we applied the two drugs on the right flanks of hairless rats for 10 days. In concentrations of 0.001-0.01 g/100 ml (ethanol) the two drugs are equally effective in inducing changes in the horny layer of the skin (increase in the amount of horny material that is able to be desquamated). This can be interpreted as a stimulation of terminal keratinocyte differentiation. However, only calcitriol caused a significant rise in the serum calcium concentration. In conclusion, calcitriol and calcipotriol seem to be equally effective after topical application on the skin of hairless rats, but calcipotriol has a much lower systemic calcium liability.

Administration, Topical↗
PubMed1991

Studies on commercial insecticides with the dominant lethal mutations test.

The dominant lethal test was performed on male Balb/c mice to examine three commercial forms of insecticide demeton, dichlorvos and endosulfan (Metasystox I forte, Nogos 50 EC and Thiodan 35, respectively). The assay was done by intraperitoneal treatment of animals both acutely (1 dose) and subacutely (5 daily administrations). The number of total implantations differed from the control value only after five-fold injection of dichlorvos. There was no statistically-significant decrease in the number of live implantations. The frequency of postimplantation lethality was no significantly increased over the control level.

Animals↗
PubMed1991

The effect of herbicide "Chwastox płynny 30" on the cell cycle parameters in root meristem of Vicia faba L. subsp. minor.

The duration of the cell cycle and its phases after the treatment with herbicide "Chwastox płynny 30" was calculated using 3H-thymidine labelling method. Inhibition of DNA synthesis and marked prolongation of G2 + 1/2 M phase were observed. Tested herbicide caused a significant lowering in the mitotic activity and accumulation of metaphase cells.

2-Methyl-4-chlorophenoxyacetic Acid↗
PubMed1989

[The use of ionizing radiation for the decontamination of salmonella-containing slaughtered broiler chickens and powdered eggs].

The effect of cobalt-60 gamma irradiation was investigated on the concentration of Salmonella (S.) typhimurium in artificial contaminated chicken carcasses and on the number of S. tennessee and S. agona in artificial contaminated spray-dried whole egg powder and liquid whole egg. Irradiation of carcasses and of liquid whole egg was carried out at deep frozen conditions between -10 degrees C and -18 degrees C and whole egg powder at 15-20 degrees C. The irradiation doses used where between 0.05 and 8.0 KGy. The D10-value of S. typhimurium in broiler chicks ranged between 0.57 and 0.74 and of S. agona respectively S. tennessee amounted to 0.95 and 1.07 in spray dried whole egg powder and 0.47 respectively 0.53 in whole egg content. Irradiation causes drastic reduction of physiological microflora in chicken carcasses. By radiation treatment using a dose of 4.0 KGy, germ count reductions by 3-5 decimal powers were achieved. Resistance of microbes increases in following succession: Pseudomonas spp., Salmonella spp., other Enterobacteriaceae spp., Micrococcus spp., Flavobacterium spp., yeasts, Bacillus spp., Streptomyces spp., fungi. Results of microbiological and sensorial examinations allow following conclusions: 1. Irradiation is highly effective against Salmonella. 2. Irradiation dose of 4.0 KGy is able to kill about 1 Million Salmonella bacteria per chicken. This guarantees safe elimination of natural occurring Salmonella bacteria in broiler chicken carcasses. 3. Irradiation of chicken carcasses up to 4.0 KGy causes no injury of quality, however storage longer than 4 months deteriorates sensorial qualities more than of unirradiated carcasses.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1989

[Recent developments and technics in eyelid-orbit surgery].

The advances in lid and orbital surgery are due to the improvements made in diagnostic equipment and to technical refinements. Modern imaging techniques now provide exact preoperative analyses, especially for orbital disorders. New suture and implant materials have been introduced, and the use of the operating microscope and of various types of lasers has broadened the spectrum of therapeutic possibilities. Surgical procedures have been developed that pay more attention to the specific anatomy, function and pathogenesis of the lids, especially for involutional disorders and loss of tissue.

Eyelid Diseases↗
PubMed1988

[Bullous dermatosis with conjunctival and corneal involvement: differential diagnostic scope and explanation of the "overlap syndrome" concept].

Chronic blister-forming dermatosis can lead to conjunctival and corneal involvement. Taking one such case as an example, the authors show that while disorders of this kind can be classified as a form of bullous dermatosis, the differential diagnostic classification is not unequivocal, regardless of the examination method adopted. The term "overlay syndrome" has been introduced into the dermatologic literature to cover clinical pictures of this kind.

Adolescent↗
PubMed1988

[Modified full-lid transplants ("tarsomarginal transplants") in reconstructive eyelid surgery].

The author first used modified full-thickness lid grafts (tarsomarginal grafts) in lid reconstruction in 1972. They consist of only conjunctiva, tarsus, and lid margin. The anterior lid lamella is rebuilt by a myocutaneous flap from the adjacent tissue. As compared to conventional full-thickness lid grafts, this reduces the risk of necrosis. No cosmetic or functional disturbance of donor eyelids was observed. In cases of total lid loss up to three tarsomarginal grafts can be inserted simultaneously. The use of modified full-thickness grafts is superior to other well-known techniques, especially in cases with defects of the medial half of the lower lid and upper lid defects, up to and including total upper lid loss, even if the tarsus in the grafts is subject to regressive changes and the lashes are usually lost.

Eyelid Neoplasms↗
PubMed1986

Induction of chromosomal aberrations in the Syrian hamster by insecticides tested in vivo.

The insecticides demeton, dimetoat, dichlorovos, endosulfan, trichlorofon, carbaryl, lindane, methoxychlor, propoxur and malathion were examined for their ability to induce chromosomal aberrations in the bone marrow cells of the Syrian hamster (Mesocricetus auratus) treated in vivo. Mutagenicity of commercial preparations was examined at four doses: LD50; 1/2, 1/5 and 1/10 LD50. The positive control was an IP injection of cyclophosphamide to hamsters at a dose of 40 mg/kg body wt. Demeton, dichlorovos and endosulfan gave positive results. Malathion, dimethoate and the mixture of methoxychlor and propoxur were weakly clastogenic; at some doses these compounds induced statistically significant increases in the number of aberrations. Trichlorfon and the mixture of carbaryl and lindane were negative in this test.

Animals↗
PubMed1986

Ovarian hyperstimulation for in-vitro fertilization controlled by GnRH agonist administered in combination with human menopausal gonadotrophins.

Surges of luteinizing hormone (LH) in serum that result in luteinization, but occur prematurely with respect to the diameter of the leading follicle, frustrate attempts to induce multiple follicular maturation for in-vitro fertilization (IVF) in a number of women. We examined the possibility of blocking premature LH surges by the administration of D-TRP6-LH-RH, a potent agonistic analogue of gonadotrophin-releasing hormone (GnRH). Six patients who had repeatedly shown premature LH surges were treated for 10 days, beginning between days 1 and 3 of the cycle with daily s.c. injections of 500 micrograms D-Trp6-LH-RH followed by a daily injection of 100 micrograms of the analogue until the day of administration of human chorionic gonadotrophin (HCG). When pituitary and ovarian suppression had occurred, ovarian stimulation with human menopausal gonadotrophin was started and adjusted in dose according to the ovarian response. HCG was injected when the dominant follicle had reached a diameter of at least 18 mm and oestradiol levels were above 300 pg for each follicle greater than 15 mm. Oocyte collection was performed 36 h later via laparoscopy, followed by IVF and embryo transfer. The six patients studied to date responded to therapy and treatment could be completed up to embryo transfer. Two patients became pregnant; one of the pregnancies, however, resulted in abortion. Combined treatment with GnRH analogue for suppression of pituitary gonadotrophin secretion followed by the administration of gonadotrophins thus seems to be a promising method for ovarian stimulation in patients who frequently exhibit premature LH discharges and therefore fail to complete treatment.

Adult↗