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Biomedical subjects

H Hübner

Publications and source records attributed to H Hübner.

At least 37 records · Page 2Linked to original sources
PubMed1996

Cholestane- and pregnane-type glycosides from the roots of Tribulus cistoides.

From the methanolic extract of the roots of Tribulus cistoides the cardioactive saponin-3, which is known to occur in the leaves, was isolated along with tribulosin, a pregnane-type glycoside and eight new cholestane glycosides. D-(+)-Pinitol and sucrose were major constituents. The structures were established by spectroscopic studies of the isolated compounds, their acetylated derivatives and their hydrolysation products. Chemical conversions revealed the configurations at C-22 and C-25, respectively.

Carbohydrate Sequence↗
PubMed1996

-Chemosensitivity testing in gynecologic oncology. Experiences with an ATP bioluminescence assay-.

In the present study, our first experiences with the ATP Tumour Chemosensitivity Assay (ATP-TCA), a novel method for pretherapeutic drug testing, are reported. During one year, a total of 111 samples obtained from patients suffering from different gynaecological malignancies (ovary: 53, breast: 41, others: 17) were sent for chemosensitivity testing. The quality of 104 single cell suspensions was sufficient for further processing. Evaluability rates ranged from 71% (breast cancers and non-ovarian tumours) to 83% (ovarian cancers) with an average of 77%. Evaluability of recurrences was slightly better compared to primaries. A total of 18 different antineoplastic agents and 67 drug combinations was used for in vitro testing with an average of 12.4 drugs/combinations tested per each sample (ovary: 14.8, breast: 9.7, others: 10.2). In 70 of 111 cases, the ATP-TCA was followed by chemotherapy with 44 cases treated in respect of the assay results and 29 cases receiving an antineoplastic regime, which differed from the standard treatment protocol. Compared to untreated patients, recurrences were treated more frequently based on the ATP-TCA, with a majority receiving an alternative protocol as proposed by the assay. In ovarian carcinoma, chemosensitivity testing was of particular importance for the further therapy. 41 of 53 patients were treated by chemotherapy with 29 therapies basing on the results of the assay (alternative protocol: 17). The good correlation of the ATP-TCA and the individual clinical course of the patients was demonstrated by two case reports of recurrent ovarian carcinomas, one of them responding well to platinum-based combinations and the other presenting in vitro and in vivo platinum-resistance. In conclusion, our first experiences with the ATP-TCA were promising. Our results, however, warrant confirmation by studies with a sufficient number of cases and an extended observation period.

Adenosine Triphosphate↗
PubMed1995

Spiganthine, the cardioactive principle of Spigelia anthelmia.

Spiganthine [1] was isolated as the main cardioactive principle from medicinally used extracts of Spigelia anthelmia. Its structure was established by spectroscopic methods. The biological effect of spiganthine is characterized by a delay in contraction development of the heart muscle.

Animals↗
PubMed1994

Cardioactive steroid saponins and other constituents from the aerial parts of Tribulus cistoides.

From the petrol extract of the aerial parts of Tribulus cistoides three steroid sapogenins and two N-acyltyramines were isolated, whereas the methanolic extract gave nine steroid saponins, among them the cardioactive cistocardin, saponin-3, saponin-4 and saponin-7. Furthermore, a furostanol diglycoside was isolated besides 5'-(hydroxysulphonyloxy) jasmonic acid, D-(+)-pinitol and sucrose. The structures were established by spectroscopic investigations of the isolated compounds and their hydrolysis products. 5'-(Hydroxysulphonyloxy) jasmonic acid has been prepared by partial synthesis.

Animals↗
PubMed1994

[Mutations of the rhodopsin gene and their phenotypic effects].

Molecular pathogenic cause of Retinitis pigmentosa leading from different mutations in rhodopsin gene and dependences between dominant or recessive mutations and clinical effects have been described. Importance of molecular analysis of rhodopsin in prognosis this disease have been emphasized.

Base Sequence↗
PubMed1994

[Resection of the Müller muscle. Indications, technique and results].

From 1987 to 1992, 582 operations for blepharoptosis were performed in our department. Twenty-five cases of superior tarsal muscle resection in 22 patients were reviewed retrospectively and serve as the basis of the present study. The blepharoptosis was mild to moderate (up to 4 mm) in 20/25 cases. Preoperatively, the phenylephrine eye drops test was positive in 20/25 cases. The indication for both congenital and acquired blepharoptosis was independent of the activity of the levator muscle. The resection was performed by a conjunctival approach. Three techniques of muscle stump refixation were tested without any significant difference in the results. A satisfactory eyelid elevation of more than 2 mm was achieved in 16/25 operations. A moderate effect (1-2 mm) was measured in 5/25 and an unsatisfactory effect (< 1 mm) in 4/25 cases. In contrast to the Fasanella-Servat procedure, the above-mentioned technique leaves all other structures of the upper eyelid intact, especially the accessory lacrimal glands.

Adult↗
PubMed1994

[Orbital exenteration. A preventable course in tumor treatment?!].

We reviewed the conditions leading to the mutilating procedure of orbital exenteration in 24 patients at our institution between 1980 and 1993. In 17 cases, including 16 of 17 patients with malignant tumours of the eyelid, this operation could potentially have been avoided. Three of these 17 patients first saw a doctor at an extremely advanced and otherwise untreatable stage of the tumour. The other 14 patients had at least one previous treatment attempt. Six patients with basal cell carcinoma had been primarily treated with radiation alone; two of them developed a squamous cell carcinoma subsequently. In six cases primary reconstruction was performed despite incomplete resection. In one patient a meibomian gland carcinoma was misdiagnosed as a chalazion and excised without histological examination. In another case of squamous cell carcinoma a cutaneous incisional biopsy 6 months prior to orbital exenteration revealed a papilloma. These results emphasize the necessity of careful histological examination in the treatment of lid tumours.

Adult↗
PubMed1992

Effects of calcipotriol (MC 903) and calcitriol after topical application on the skin of hairless rats. Much lower effect of calcipotriol on systemic calcium homeostasis.

Topical application of calcitriol or its analogs is a new approach for treating psoriasis, but may be limited by systemic calcitropic effects. Calcipotriol (MC 903) is a novel calcitriol analog with low calcitropic potency after systemic application. To compare the topical potency of calcitriol and calcipotriol we applied the two drugs on the right flanks of hairless rats for 10 days. In concentrations of 0.001-0.01 g/100 ml (ethanol) the two drugs are equally effective in inducing changes in the horny layer of the skin (increase in the amount of horny material that is able to be desquamated). This can be interpreted as a stimulation of terminal keratinocyte differentiation. However, only calcitriol caused a significant rise in the serum calcium concentration. In conclusion, calcitriol and calcipotriol seem to be equally effective after topical application on the skin of hairless rats, but calcipotriol has a much lower systemic calcium liability.

Administration, Topical↗
PubMed1991

Studies on commercial insecticides with the dominant lethal mutations test.

The dominant lethal test was performed on male Balb/c mice to examine three commercial forms of insecticide demeton, dichlorvos and endosulfan (Metasystox I forte, Nogos 50 EC and Thiodan 35, respectively). The assay was done by intraperitoneal treatment of animals both acutely (1 dose) and subacutely (5 daily administrations). The number of total implantations differed from the control value only after five-fold injection of dichlorvos. There was no statistically-significant decrease in the number of live implantations. The frequency of postimplantation lethality was no significantly increased over the control level.

Animals↗
PubMed1991

The effect of herbicide "Chwastox płynny 30" on the cell cycle parameters in root meristem of Vicia faba L. subsp. minor.

The duration of the cell cycle and its phases after the treatment with herbicide "Chwastox płynny 30" was calculated using 3H-thymidine labelling method. Inhibition of DNA synthesis and marked prolongation of G2 + 1/2 M phase were observed. Tested herbicide caused a significant lowering in the mitotic activity and accumulation of metaphase cells.

2-Methyl-4-chlorophenoxyacetic Acid↗
PubMed1989

[The use of ionizing radiation for the decontamination of salmonella-containing slaughtered broiler chickens and powdered eggs].

The effect of cobalt-60 gamma irradiation was investigated on the concentration of Salmonella (S.) typhimurium in artificial contaminated chicken carcasses and on the number of S. tennessee and S. agona in artificial contaminated spray-dried whole egg powder and liquid whole egg. Irradiation of carcasses and of liquid whole egg was carried out at deep frozen conditions between -10 degrees C and -18 degrees C and whole egg powder at 15-20 degrees C. The irradiation doses used where between 0.05 and 8.0 KGy. The D10-value of S. typhimurium in broiler chicks ranged between 0.57 and 0.74 and of S. agona respectively S. tennessee amounted to 0.95 and 1.07 in spray dried whole egg powder and 0.47 respectively 0.53 in whole egg content. Irradiation causes drastic reduction of physiological microflora in chicken carcasses. By radiation treatment using a dose of 4.0 KGy, germ count reductions by 3-5 decimal powers were achieved. Resistance of microbes increases in following succession: Pseudomonas spp., Salmonella spp., other Enterobacteriaceae spp., Micrococcus spp., Flavobacterium spp., yeasts, Bacillus spp., Streptomyces spp., fungi. Results of microbiological and sensorial examinations allow following conclusions: 1. Irradiation is highly effective against Salmonella. 2. Irradiation dose of 4.0 KGy is able to kill about 1 Million Salmonella bacteria per chicken. This guarantees safe elimination of natural occurring Salmonella bacteria in broiler chicken carcasses. 3. Irradiation of chicken carcasses up to 4.0 KGy causes no injury of quality, however storage longer than 4 months deteriorates sensorial qualities more than of unirradiated carcasses.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1989

[Recent developments and technics in eyelid-orbit surgery].

The advances in lid and orbital surgery are due to the improvements made in diagnostic equipment and to technical refinements. Modern imaging techniques now provide exact preoperative analyses, especially for orbital disorders. New suture and implant materials have been introduced, and the use of the operating microscope and of various types of lasers has broadened the spectrum of therapeutic possibilities. Surgical procedures have been developed that pay more attention to the specific anatomy, function and pathogenesis of the lids, especially for involutional disorders and loss of tissue.

Eyelid Diseases↗
PubMed1988

[Bullous dermatosis with conjunctival and corneal involvement: differential diagnostic scope and explanation of the "overlap syndrome" concept].

Chronic blister-forming dermatosis can lead to conjunctival and corneal involvement. Taking one such case as an example, the authors show that while disorders of this kind can be classified as a form of bullous dermatosis, the differential diagnostic classification is not unequivocal, regardless of the examination method adopted. The term "overlay syndrome" has been introduced into the dermatologic literature to cover clinical pictures of this kind.

Adolescent↗
PubMed1988

[Modified full-lid transplants ("tarsomarginal transplants") in reconstructive eyelid surgery].

The author first used modified full-thickness lid grafts (tarsomarginal grafts) in lid reconstruction in 1972. They consist of only conjunctiva, tarsus, and lid margin. The anterior lid lamella is rebuilt by a myocutaneous flap from the adjacent tissue. As compared to conventional full-thickness lid grafts, this reduces the risk of necrosis. No cosmetic or functional disturbance of donor eyelids was observed. In cases of total lid loss up to three tarsomarginal grafts can be inserted simultaneously. The use of modified full-thickness grafts is superior to other well-known techniques, especially in cases with defects of the medial half of the lower lid and upper lid defects, up to and including total upper lid loss, even if the tarsus in the grafts is subject to regressive changes and the lashes are usually lost.

Eyelid Neoplasms↗
PubMed1986

Induction of chromosomal aberrations in the Syrian hamster by insecticides tested in vivo.

The insecticides demeton, dimetoat, dichlorovos, endosulfan, trichlorofon, carbaryl, lindane, methoxychlor, propoxur and malathion were examined for their ability to induce chromosomal aberrations in the bone marrow cells of the Syrian hamster (Mesocricetus auratus) treated in vivo. Mutagenicity of commercial preparations was examined at four doses: LD50; 1/2, 1/5 and 1/10 LD50. The positive control was an IP injection of cyclophosphamide to hamsters at a dose of 40 mg/kg body wt. Demeton, dichlorovos and endosulfan gave positive results. Malathion, dimethoate and the mixture of methoxychlor and propoxur were weakly clastogenic; at some doses these compounds induced statistically significant increases in the number of aberrations. Trichlorfon and the mixture of carbaryl and lindane were negative in this test.

Animals↗