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Biomedical subjects

H Hager

Publications and source records attributed to H Hager.

At least 55 records · Page 3Linked to original sources
PubMed1987

Quantification of CD8-positive lymphocytes in lymph node follicles from HIV-infected male homosexuals and controls.

The number of CD8-positive cells in follicular centres of hyperplastic lymph nodes from 20 Danish and Swedish homosexual men with persistent generalized lymphadenopathy and 43 control patients were enumerated in frozen tissue sections immunostained with monoclonal antibody reactive with the CD8-antigen ("cytotoxic-suppressor" T-cell antigen). All the homosexuals were seropositive for HIV and histology showed changes characteristic of the early stage of HIV lymphadenitis. A significant increase (p much less than 0.001) of CD8-positive cells was demonstrated (mean 1,307 per mm2 follicular centre, SD 639) in HIV-related lymphadenopathy compared with the controls (mean 161 CD8-positive cells per mm2 follicular centre, SD 169). The results of this study show that the immunohistological demonstration of a significant increase of CD8-positive cells in the follicles of hyperplastic lymph nodes is suggestive of HIV-related lymphadenopathy.

Acquired Immunodeficiency Syndrome↗
PubMed1982

Epidemiology of an outbreak in a maternity unit of infections with an antigenic variant of Echovirus 11.

After an 8-day-old child had died with clinical signs of septicemia, 6 other newborns fell ill. Virus was isolated from various sites from all the 6 children (28 isolations). The agent was identified by cross neutralization tests as an antigenic variant of Echovirus 11. The agent could not be isolated from mothers or nursery staff (49 people). We therefore tried to trace the path of infection by isolating specific IgM and IgG antibodies. A laboratory infection by the agent isolated enabled the time pattern of the serologic immune response to be roughly determined. The data collected indicate that the infection spread through close contact between the affected newborns and nurses working in the newborn room. Rigorous hygienic and isolation measures, initiated immediately, appeared to interrupt the spread of infection.

Antibodies, Viral↗
PubMed1982

Alanine aminotransferase in bovine brain: purification and properties.

Mitochondrial and cytosolic alanine aminotransferases (EC 2.6.1.2) were partially purified (140- and 180-fold), respectively) from bovine brain cortex by means of (NH4)2SO4 precipitation, gel filtration on Sephadex G-150, and in-exchange chromatography on DEAE A-50 and characterized. The enzymes exhibited identical molecular weights (110,000 +/- 10,000) and pH optima (7.8), but were eluted from CM Sephadex C-50 at different ionic strengths. Isoelectric focusing of the enzymes indicated a pI value of 5.2 for the cytosolic enzyme and 7.2 for the mitochondrial enzyme. The Km values of the mitochondrial enzyme were 5.1 mM, 6.6 mM, 0.7 mM, and 0.4 mM and of the cytosolic isozyme were 30.3 mM, 4.3 mM, 0.7 mM, and 0.5 mM for alanine, glutamate, 2-oxoglutarate, and pyruvate, respectively. The results indicated that two forms of alanine aminotransferase exist in nerve tissue, which suggests that they may play different roles in the cellular metabolism of nerve tissue.

Alanine Transaminase↗
PubMed1981

Alanine aminotransferase and some other enzymes in different populations of free brain cortex mitochondria.

The activity of certain key enzymes involved in glutamic acid metabolism was studied in purified brain mitochondria and in mitochondrial subfractions separated in a discontinuous 1.2--1.6 mol/l sucrose gradient. Alanine aminotransferase and glutamate dehydrogenase were found to be matrix enzymes and aspartate aminotransferase to be associated with the inner mitochondrial membranes. After the purified mitochondria had been separated into 5 subfractions, aspartate aminotransferase and NAD+-dependent isocitrate dehydrogenase were found to be bound to the lighter mitochondrial subfractions settling at the 1.4--1.5 mol/l sucrose boundary while alanine aminotransferase, 4-aminobutyrate transaminase and glutamate dehydrogenase were associated with the heavier subfractions settling below 2.4 mol/l sucrose. The highest specific activity of the given enzymes was found in the subfraction settling at the 1.4--1.5 mol/l sucrose boundary, the only exception being alanine aminotransferase activity, whose maximum was found in the subfractions settling in 1.5 and 1.6 mol/l sucrose. It was concluded that alanine aminotransferase, in conjunction with glutamate dehydrogenase, is linked to NH3 binding and to the oxidation of reduced adenine nucleotides; in addition, alanine aminotransferase is presumed to have the function of transporting glutamate from the mitochondria to the extramitochondrial space.

4-Aminobutyrate Transaminase↗
PubMed1981

Immunochemical determination of CK-MB isoenzyme in human serum: a radiometric approach.

The presence of creatine kinase isoenzyme MB (CK-MB) in human serum is an indicator of myocardial injury. In this assay for CK-MB, reagent containing both rabbit antibodies to CK-MM and 125I-labeled sheep antibodies to CK-BB is added to the patient's serum and incubated for 1 h at ambient temperature. Goat anti-rabbit immunoglobulin, conjugated to a mixture of amylose and polyvinylidene fluoride floccules, is then added. After a 15-min incubation, the mixture is centrifuged, isolating the two M-subunit-containing isoenzymes as insoluble complexes. Because the B-subunit portion of MB is labeled with 125I, the radio-activity in the pellet will be proportional to the amount of MB present. The discarded supernate contains excess sheep 125I-labeled BB antibodies, free or bound to BB isoenzyme if it is present. The preparation and characterization of the isoenzymes and antibodies is explained. Concentrations of CK-MB is sera of patients with and without an acute myocardial infarction were assayed in serial specimens obtained in 103 consecutive admissions to a coronary care unit. The performance of this radiometric procedure compared well with CK-MB electrophoresis, giving a sensitivity of 100% and a specificity of 92%.

Brain↗
PubMed1980

[An epidemic in a maternity unit caused by an echo virus 11 variant (author's transl)].

In October 1978, a 6 day-old child became ill in a maternity ward and died with clinical signs of septicemia. In quick succession, 6 other children fell ill with aseptic meningitis, 5 within the first 10 days post partum and one child 4 weeks old. From these 6 children virus was isolated at different times and from various sites (CSF, pharynx, rectum). 25 isolates were obtained. By cross-neutralization an antigenic variant of Echo virus 11 was identified. Since further isolations were unsuccessful the route of infection was reconstructed from environmental investigation and clinical and serological data in 49 people. A laboratory infection with the agent isolated gave additional opportunities for detailed virological and clinical observations.

Disease Outbreaks↗
PubMed1980

[Treatment of central retinal venous and arterial occlusion by isovolemic hemodilution (author's transl)].

(1) Ten patients (mean age 71) with fully developed central retinal venous occlusion were treated by isovolemic hemodilution over a period of four to six weeks. Hematocrit was lowered in steps to about 30% to improve retinal microcirculation. For two to four days, 500 ml whole blood were exchanged daily for about 250 ml hydroxyethyl starch and 250 ml plasma. The procedure was repeated every five to ten days whenever the hematocrit exceeded 35%. Visual acuity was considered to be improved when the patient could read at least two more lines on the test card. Four to six weeks after starting hemodilution therapy visual acuity had improved in seven patients, deteriorated in one, and was unchanged in two. In the follow-up study six months after treatment visual acuity, as compared to data before treatment, had improved in four patients, deteriorated in two and was unchanged in four. Three patients developed a neovascular glaucoma and had to undergo photocagulation treatment. (2) As expected, hemodilution failed to improve visual acuity in five patients who had had a complete central retinal arterial occlusion for more than four hours. (3) This is the first time that a beneficial effect of isovolemic hemodilution on visual acuity, probably mediated by an improvement in retinal blood flow, has been demonstrated in man. However, only in retinal tissue with at least minimal blood flow (venous but not arterial occlusion) can this method be effective. Except for severe anemia or thrombocytosis there are no absolute contraindications. No adverse side-effects were observed. To prevent neovascularization it is planned to use a combination of hemodilution and photocoagulation.

Aged↗
PubMed1979

Semliki forest virus: cause of a fatal case of human encephalitis.

A fatal case of human encephalitis has been observed for which our results indicate that Semliki Forest virus (SFV) was the etiologic agent. This is surprising in view of the fact that this virus, which has been widely studied, was believed to be one of the arboviruses nonpathogenic for man. Described are the clinical course, the virological examinations performed, and the histopathological findings in the central nervous system.

Adult↗
PubMed1979

[Peripheral nerve changes in Tangier disease. Comparative light-, electron-microscopic and cytometric studies (author's transl)].

A sural nerve biopsy was performed in a 55-year-old male patient with Tangier disease (familial-lipoprotein deficiency). Light-microscopy showed an increase in the endoneural connective tissue and a loss of nerve fibers indicating a chronic peripheral neuropathy. Electron-microscopy revealed an accumulation of lipid droplets within Schwann cells of myelinated and unmyelinated nerve fibers. When compared with age-matched controls the myelinated fiber density was reduced with a relative preponderance of small myelinated fibers. In addition, distributional cytometric studies of nerve fibers in relation to the perineurium and endoneurial capillaries showed: Contrary to 4.6-7.5 micron thick nerve fibers, which accumulated in the center of the nerve fascicle, small (0.5-4.5 micron) and large (7.6-10.0 micron) fibers lay nearby the perineurium. The measured increase in small myelinated nerve fibers around endoneurial capillaries may be explained as a sign of regeneration.

Connective Tissue↗
PubMed1979

[Clinical and experimental evaluation of hydrophilic contact lenses for continuous-wear (author's transl)].

In a clinical and experimental study we tested a new contact lens for continuous-wear. The modified HEMA-lens ("Permalens", Global Vision Ltd, Southampton/GB; Cooper Laboratories, Düsseldorf) has a water content of 74% at pH 7.4. The oxygen transmissibility is, depending on the thickness of the lens, 38--67% of the oxygen in the air. In a first trial we had to eliminate 6 patients due to wrong fitting (much too steep) or dry eyes. After development of a new guide for selection of lens base curves 10 patients with monocular aphakia (10 eyes) and 12 patients with mild myopia (24 eyes) were fitted with the hydrophilic soft contact lens for permanent wear. From a total of 34 eyes in 11 cases (32%) the lenses were removed due to complications (1 conjunctivitis, 1 keratitis, 1 vascularization) or due to lack of cooperation. In 23 cases (68%) the continuous-war lenses were well tolerated without complications for 5 months. In a comparative study in vitro we tested the effect of the contact lens on the viability (swelling rate, intracellular membrane potential of the epithelium) of the isolated human cornea. A damaging effect of the lens on corneal function was not observed. The hydrophilic soft contact lens can be fitted for continuous-wear.

Adult↗
PubMed1979

[Vaginal exstirpation of uterus following gynaecological or obstetrical laparotomies (author's transl)].

Of a total of 3,213 vaginal exstirpations of the uterus, this operation was performed in 132 women following gynaecological laparotomies. In spite of this, the incidence of complications was not increased. It is concluded that the advantage of the vaginal route of operation in hysterectomies need not be refused to patients in whom laparotomies have been performed if certain conditions are observed.

Cesarean Section↗
PubMed1977

[Lasermicrosurgery of the flattened anterior chamber (author's transl)].

The method of laser-microsurgery for restoration of lost anterior chamber in aphakic pupillary block and malignant glaucoma is once more reported. The problems of the flattened anterior chamber are discussed. Principle, technique, and indication of laser-coagulation of the iris are demonstrated. A short discussion of the method, description of late observations and instructions for postoperative treatment are given. In each of the 23 eyes this dangerous postoperative complication could be abolished in a simple manner without any risk. This operation must be considered as the method of choice.

Anterior Chamber↗