Biomedical subjects
Publications and source records attributed to H Heymann.
Sucrose-lysozyme spheroplasts were prepared from two strains of Pseudomonas cepacia and tested for susceptibility to polymyxin B and benzalkonium chloride. Spheroplasts were more susceptible than whole cells to benzalkonium chloride but not to polymyxin B. Disruption of the outer membrane layer was not by itself sufficient to render P. cepacia susceptible to polymyxin B.
By combining different methods available in modern gastric surgery, we have developed a new procedure in surgical treatment of the complicated ulcer of the posterior duodenal wall. Our new technique allows the radical resectioning of the ulcer and includes all the advantages of nonresectioning operations in form- and function-preserving surgery. The technique of open anastomosis with an one-row, one-layer suture only slightly injures the gut that is to be anastomosed and thus eliminates complications in the healing process. The pylorus is completely preserved. The great advantages of avoiding gall reflux into the stomach, of preserving its total capacity, and of preserving the possibility of a portioned evacuating of the stomach can not yet be fully estimated.
The construction and use of a new cannula for the long-term cannulation of the stomach in experimental surgery is described. The major attribute of the gastric fistula is the simplicity of technical management and the advantage to avoid irritation of the animals as much as possible.
Comparison of various methods of collecting pancreatic juice in long-term experiments shows that there is no ideal procedure. The authors themselves obtained the best results by help of the duodenal cannula according to Tuckermann and Thomas, which is implanted in the duodenum opposite to the duodenal papilla. Using this cannula it is often possible with sufficient skill and patience to cannulate the duodenal papilla from the outside and to gain pancreatic juice from the awake dog in an exact manner. Among the pouch techniques the best is the method according to Herrera.
Selective or selective-proximal vagotomy has increased in preference as surgical treatment for uncomplicated duodenal ulcer disease. An unusual immediate complication of transabdominal vagotomy is the hemothorax. Few cases of this complication have been reported in literature. Our case represents a variation with simultaneous presence of a both-sided hemothorax. Although the exact etiology of this complication remains obscure, we try to explain this spontaneous hemothorax by operative injuries of subpleural vessels.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Cells of Pseudomonas aeruginosa resistant to benzalkonium chloride (BC) underwent unique ultrastructural reorganizations when they were grown in the presence of 1 mg of BC/ml. The resistant cells usually contained a single, centrally positioned pseudovacuole. The pseudovacuole was surrounded by a diffuse substance that spread irregularly throughout the cytoplasm. The presence of the pseudovacuole seemed to cause a physical compartmentalization of the cytoplasm into random pockets of ribosomes and nuclear material. Contained within the pseudovacuole was a horseshoe-shaped, electron-dense body which was bounded by a trilaminar membrane 5.2 nm in width. These bodies averaged 77 nm when measured through the long axis. The surfaces of resistant cells were covered by an additional layer not found in sensitive cells. Thin sections of sensitive cells which had been treated with 1 mg of BC/ml showed little or no lysis. The cytoplasm appeared to be deeply stained and coagulated. Ribosomes were no longer distinctly visible. Although the cell wall remained intact, the cell membrane was dissolved and fragmented. BC-grown resistant cells could not be successfully stained by standard techniques; however, details were demonstrated with the aid of a combination of 1.5% glutaraldehyde, 1% osmium tetroxide, and 1% phosphotungstic acid prepared in 0.1 m sodium dimethylarsonate buffer (pH 6.8).
The use of a combination fixative-staining procedure has allowed a detailed observation of mesosomes in thin sections of Pseudomonas aeruginosa.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.