Glucose-1-(14)C and acetylglucosamine-1-(14)C were added singly and together with equal amounts of the unlabeled reciprocal to Brain Heart Infusion and used for the culture of Streptococcus pyogenes. The labeling pattern of the rhamnose, glucosamine, and muramic acid in the cell wall supported an intermediary role for acetylglucosamine in providing the C1-C6 moiety of muramic acid. Although radioactivity in the C2-C9 portion of muramic acid suggested that some of the lactyl group (C7-C9) came from glycolytic products, there was also considerable contribution to it from noncarbohydrate sources. Using cell-free extracts, we were unable to demonstrate biosynthesis of acetylmuramic acid, either free or nucleotide-bound, while glycolysis was occurring. The formation of uridine diphosphoacetylmuramic acid has been reported by others who used uridine diphospho-N-acetyl-d-glucosamine, phosphoenolpyruvate, reduced nicotinamide adenine dinucleotide, and reduced nicotinamide adenine dinucleotide phosphate. However, we did not detect the formation of this compound.

Acids↗

PubMed1966

Preparation and antibacterial activity of some spiramycin derivatives.

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Chemistry, Organic↗

PubMedDate not supplied

[Experimental study of the local application of silver sulfadiazine, cefsulodin and povidone-iodine in burns].

Non-infected standardized burns and those contaminated experimentally with a constant number of organisms of a selected Pseudomonas aeruginosa strain underwent varying forms of treatment with silver sulfadiazine, cefsulodin cream and povidone iodine ointment. Wound healing was controlled by evaluating the wound area. In burns which had not been infected experimentally, healing was best without any treatment. Burns treated with cefsulodin cream showed delayed healing, though this was not significant. The most significant delay, however, was observed in wounds treated with povidone iodine. Wounds infected with Pseudomonas healed considerably better than the control group when treated prophylactically with silver sulfadiazine and cefsulodin. However, burns treated with povidone iodine again showed delayed healing.

Administration, Topical↗