Biomedical subjects

H Inatomi

Publications and source records attributed to H Inatomi.

40 records · Page 3Linked to original sources

PubMedDate not supplied

Suppression by coffee cherry of the growth of spontaneous mammary tumours in SHN mice.

We previously found that coffee cherry (CC), residue after removal of coffee beans, significantly suppressed the development of spontaneous mammary tumours of mice. In this paper, the effects of CC on the growth of the palpable size of this type of tumour was examined. Free access as drinking water of 0.5% solution of the hot water extract of CC for 10 days resulted in a marked inhibition of the tumour growth: The percent changes of tumour sizes were 53.8 +/- 11.7% and 13.8 +/- 10.9% in the control and the experimental groups, respectively. Associated with this, thymidylate synthetase activity in the mammary tumours was significantly lower in the experimental group than in the control. Normal and preneoplastic mammary gland growth, body weight change and weights and structures of endocrine organs were only slightly affected by the treatment. The findings indicate that CC is promising as an antitumour agent.

Adrenal Glands↗

PubMedDate not supplied

Further study on the effects of coffee cherry on spontaneous mammary tumourigenesis in mice: effects of methanol extract.

To examine the effective components in the antitumourigenic role of water extract of coffee cherry (CC), and the residue after removal of beans, the dry matter of coffee cherry was extracted repeatedly by 60% methanol and further divided into water-soluble and -insoluble fractions (CC-W and CC-M, respectively). Free access to CC-W (0.25% in tap water) markedly inhibited mammary tumour development in SHN virgin mice, while it had no effect on the growth of normal and preneoplastic mammary glands and established tumours. Associated with this, urinary excretion of some components and intrathymic T cell differentiation were stimulated by the treatment with the CC. CC-W had little affect on the endocrine system, including serum prolactin level, oestrous cycles and endocrine organs, but retarded ovarian degeneration in mice developing mammary tumours. The finding in CC-W were generally similar to those in CC. Fat emulsion of CC-M, which was only checked for mammary tumour growth owing to the limited amount available, showed no effect at the dose level used (0.25% in drinking water). Finally, the previous and the present findings taken together strongly suggest that the effective components of coffee cherry are in the water-soluble fraction.

Analysis of Variance↗

PubMedDate not supplied

Effects of aristoloside, a component of guan-mu-tong (Caulis aristolochiae manshuriensis), on normal and preneoplastic mammary gland growth in mice.

Aristoloside, an extract of Guan-mu-tong (Gmt; Caulis aristolochiae manshuriensis) showing a marked anticancer potential, was dissolved with tap water (0.05%) and given to 4 month-old SHN mice for 30 days as drinking water. The treatment induced the inhibition of preneoplastic mammary gland growth and the stimulation of the excretion of urinary component. Ovarian follicles were dominant and the oestrous stage was elongated by the treatment. Aristoloside had little affect on normal mammary gland growth and serum levels of prolactin and free fatty acid. All findings were in agreement with those obtained from studies on chronic treatment with Gmt, and aristoloside is thus strongly suggested as being one of the major components of Gmt.

Animals↗

PubMedDate not supplied

Inhibition by guanidino compounds of the growth of spontaneous mammary tumours in SHN mice.

The D-form of lombricine extracted from the earthworm was previously found to inhibit the growth of spontaneous mammary tumours of mice. In the present study, the anti-mammary tumour effects of guanidino compounds, of which structures resembled those of lombricine, i.e., guanidinesulfate (Guanidine), 2-guanidinoethanol sulfate (2-GEt), 4-guanidino-1-butanol sulfate (4-GBt), guanidinoethylphosphate (GEP), opheline (OPHE) and the L-form of lombricine (L-Lom), were investigated in relation to their structures. In Experiment I, each guanidino compound was suspended in olive oil at the concentration of 0.3 mg/0.05 ml, and was injected subcutaneously for 10 days to SHN mice bearing spontaneous mammary tumours. 2-GEt, GEP, OPHE and L-Lom markedly inhibited mammary tumour growth, but Guanidine and 4-GBt did not. In Experiment II, the the compounds examined, 2-GEt and GEP, also significantly inhibited mammary tumour growth when given in drinking water (0.003%) for 10 days, while the effects were much weaker than those of the injected compounds. These results indicate that the existence and the length of the ethylene group are important to antitumour activity and that the site of the antitumour activity in guanidino compounds is the guanidinoethyl group. These compounds are also suggested to be more effective when administered subcutaneously rather than intragastrically.

Animals↗