Biomedical subjects

H Kemona

Publications and source records attributed to H Kemona.

At least 19 recordsLinked to original sources

PubMed2005

Soluble P-selectin concentration in patients with colorectal cancer.

During platelet activation, P-selectin is translocated onto the external platelet membrane. Surface exposure of P-selectin is temporary, and the molecule undergoes endocytosis or shedding to the circulation where it appears in a soluble form as sP-selectin. The aim of the study was to assess platelet activation based on the level of soluble form of P-selectin (sP-selectin) in colorectal cancer patients. The study involved 22 surgically treated patients, divided into two groups depending on histopathological malignancy grade: group I - patients with low malignancy grade (G2), group II - patients with high malignancy grade (G3). The examinations were carried out three times: before surgery (A0) and 3 (A1) and 12 days (A2) after the surgery. Control group (C) consisted of 20 healthy subjects. The sP-selectin level was determined in the plasma using the ELISA Kit (Human sP-selectin, R&D System). In colorectal cancer patients sP-selectin concentration was statistically significantly higher (69.25 ng/ml in group I and 66.50 ng/ml in group II) as compared to healthy subjects (46.01 ng/ml) (p<0.05), irrespective of malignancy grade. The surgical procedure has a significant effect on the dynamics of changes in the level of sP-selectin. Initially, 3 days (A1) after the procedure, there is a decrease in the level of sP-selectin, but after 12 days (A2) a rise is noted again, the level being the highest in group I. It indicates that the surgical procedure does not totally eliminate the factors responsible for platelet activation and did not normalize platelet activation.

Adenocarcinoma↗

PubMed2005

Phagocytic and bactericidal activity and morphological parameters of blood platelets in patients with Trichinella spiralis infection.

PURPOSE: The production of IgE increases in parasitic invasions, triggering local or systemic inflammatory response with the involvement of blood platelets. The aim of the study was to assess the number and morphological parameters of blood platelets as well as their phagocytic and bactericidal activity in patients with Trichinella spiralis infection. It is interesting to investigate the blood platelet response following Trichinella spiralis in order to elucidate possible effects on non-specific immunity. MATERIAL AND METHODS: Twenty-six patients with Trichinella spiralis (before and after antiparasitic therapy) and forty healthy subjects were examined. The platelet count and morphological parameters were determined using a hematologic analyzer Technicon H-1 System. The platelet phagocytic activity was determined by measuring the percentage of phagocytizing cells and the phagocytic index. The bactericidal activity was assessed measuring the percentage of the bacteria killed by platelets and plasma. The strain Staphylococcus aureus ATCC 6538P was used for this purpose. RESULTS: In patients infected with T. spiralis morphological parameters do not change, except for the percentage of large platelets. In the course of trichinellosis the phagocytic index of platelets is statistically significantly decreased and platelet bactericidal activity is impaired, while the bactericidal activity of the plasma is statistically significantly increased, compared to healthy subjects. CONCLUSIONS: The present study has revealed that due to T. spiralis infection, the percentage of large, young blood platelets is decreased. The parasitic infection causes impairment of non-specific immunity through decreased bactericidal activity of blood platelets.

Adult↗

PubMed2004

Bactericidal capacity of platelets in gastric cancer patients.

The aim of this study was to evaluate bactericidal capacity of platelets in patients suffering from gastric cancer. Number of platelets and their bactericidal activity were measured in 32 cancer patients (divided into 2 groups: I--resectable cancer, II--non-resectable one) and 32 normal donors. In group I the number of platelets was 259.136+/-84.459 x 103/microl. It was increased comparing to the normal donors 193.219+/-55.493 x 103/microl. After the surgery increase in platelet number was observed (472.05+/-111.772 x 103/microl). In group II an increased number of platelets was observed (265.1+/-81.813 x 103/microl) and it was maintained in a post-operative period: 234.2+/-54.141 x 103/microl. In group I bactericidal capacity of platelets was 2.25+/-7.33%, whereas it increased significantly after the surgery--4.7+/-7.46%. In group II, it was 8.6+/-17.61% before and 4.72+/-4.76% after the surgery. In normal donors this ability was 21.66+/-16.66. In gastric cancer patients increased platelet number was observed. Significant increase in platelets number occurred after a radical tumor removal. Decreased bactericidal activity of platelets was noticed in gastric cancer patients. After surgical removal of the tumor, platelets partly reclaimed bactericidal capacity. In patients presenting disseminated gastric cancer, bactericidal capacity of platelets could be permanently impaired.

Aged↗

PubMed2003

Effect of tumor stage and nephrectomy on CD62P expression and sP-selectin concentration in renal cancer.

The CD62P receptor and its soluble form sP-selectin is a marker of platelet (PLT) activation, and constitutes a ligand for CD24 antigen of neoplastic cells and tumor stroma components. The aim of the study was to evaluate the relationship dynamics of the percentage of CD62P+ platelets, the level of the receptor expression and the concentration of soluble form of sP-selectin in renal cancer. Examinations were performed before and after nephrectomy in patients with renal cancer (group A - 25, T2N0M0; group B - 27, T2N1-2M0) and in control group (C - 24 subjects). The two groups A and B showed an increased subpopulation of CD62P+ platelets (p<0.01) and elevated sP-selectin concentration (p<0.001) before and after nephrectomy. Although following nephrectomy sP-selectin concentration decreased markedly, it was still higher 3 months after the procedure compared to control group (p<0.05). Following nephrectomy, however, no statistically significant differences were found in the % of CD62P+ platelets and the receptor expression. Greater dynamics of changes before and after nephrectomy in the percentage of CD62P+ platelets (B1:B2 p<0.05) and the receptor expression (B1:B3 p<0.001) was observed in patients with local lymph node involvement (group B) while sP-selectin concentration was similar in both groups. Nephrectomy did not normalize intravascular activation of PLT and TNM had no significant effect on the expression of CD62P and concentration of sP-selectin.

Adult↗

PubMed2002

Does renal carcinoma affect the expression of P-selectin on platelets?

We studied changes in the expression of P-selectin on the blood platelet plasma membrane. The aim of the study was to determine the influence of renal carcinoma on P-selectin expression associated with changes in platelet morphology. Venous blood was collected from 30 patients with renal carcinoma and from 24 control subjects for cytometric analysis and to evaluate platelet morphology. P-selectin being the CD62P receptor on blood platelets was marked by anti-CD61/62P MoAb, and the results were presented as the percentage of CD62P-positive cells. Changes in the expression of the CD62P on the platelet plasma membrane during activation were investigated by flow cytometry in a comparative study of in vivo activation and in vitro platelet reactivity. Platelet activation reflected by P-selectin expression was higher in the group of patients (4.45 +/-1.96), compared to control (2.48 +/-1.66) (p < 0.05). However, adenosine diphosphate [ADP] -stimulated platelet reactivity in renal cancer patients increased only by 0.24% (p > 0.05), while following activation by thrombin by 0.54% (p < 0.05). Moreover, a higher (4.72 +/-2.02), statistically significant percentage of platelets with P-selectin expression was found in patients with disseminated neoplastic changes in renal parenchyma, compared to patients with a single localized neoplastic lesion (4.17 +/-1.89) (p < 0.05). A statistically significant difference was noted in the platelet count and anisocytosis in renal cancer patients. Renal cancer enhances P-selectin expression. It is due to the presence of intensified thrombinogenesis and other platelet agonists in the blood.

Adult↗

PubMed2001

Does parasitic infection affect platelet factor 4 concentration?

Blood platelets actively participate in defense mechanisms of the organism, e.g. in antiparasitic immunity. Platelet stimulation may be a result of direct contact with parasite, increased IgG and/or IgE concentrations, the presence of complement, CRP or lymphokins. The aim of the study was to find out whether parasitic infection induces platelet activation. The study involved 35 patients (30 patients were infected with Giardia intestinalis and 5 patients were infected with Echinococcus granulosus). Blood for analysis was collected twice, before treatment (A1) and after it (A2). The concentrations of platelet factor 4 were assayed using a set of ASSERACHROM according to the immunoenzymatic method with labelled antibodies. The mean concentration of platelet factor 4 (PF4) was 20.3 +/- 9.4 IU/ml in the patients prior to the antiparasitic therapy and being reduced to 6.0 +/- 3.0 IU/ml after treatment. In the control group the mean PF4 concentration was 2.27 +/- 0.08 IU/ml. The differences between the values in control group and the two groups tested were statistically significant. Although platelets do not get into a direct contact with the parasite, an increase is observed in the concentration of platelet factor 4, which may indicate the involvement of platelets in the parasitic disease.

Adult↗

PubMed2001

beta-Thromboglobulin in patients with acute myocardial infarction according to the treatment.

Many studies report on the increased activity of platelets in patients with heart disease, especially with acute myocardial infarction (MI). The aim of the present study was to evaluate dynamics of beta-thromboglobulin (beta-TG) concentration in patients with MI according to the disease duration and type of treatment. We investigated 29 patients, who were divided into two groups according to the type of treatment. beta-TG concentration was determined using the immunoenzymatic method (ELISA). Blood was taken 1st, 3rd, 5th, 8th and 11th day of infarction. Our results indicate that in the course of myocardial infarction there is a change in platelet activation. The treatment applied affects beta-TG concentration, and the thrombolytic therapy inhibits platelet activation.

Aspirin↗

PubMed2001

[Evaluation of functional state of blood platelets in patients infected with Echinococcus granulosus].

Blood platelets actively participate in defense mechanisms of the organism. eg. in antiparasitic immunology. The study involved 17 patients infected with Echinococcus granulosus. Blood for analysis was collected twice, before treatment (E1) and after it (E2). Control group consisted of 30 healthy people. The concentrations of beta-thromboglobulin and platelet factor 4 were assayed using a set of ASSERACHROM (Boehringer Mannheim) according to the immunoenzymatic method with labelled antibodies. The level of platelet factor 4 (before and after the antiparasitic treatment) indicates platelet activation by parasite.

Adolescent↗

PubMed2000

alpha1-microglobulin as a marker of proximal tubular damage in urinary tract infection in children.

The urinary alpha1-microglobulin (alpha1-M) as a marker of proximal tubular damage was measured in 86 children, aged 3/12 to 12 years, with a diagnosis of urinary tract infection (UTI) and fever. All patients had normal glomerular filtration rates (GFR). They were divided into 2 groups: A: with UTI and etiological factor E. coli, B: with UTI and etiological factor Proteus sp. Similar measurements of alpha1-M were obtained for a control group of healthy children. An increased mean level serum alpha1-M was observed in patients with UTI and fever compared to control group (p < 0.001). Urinary alpha1-M as the alpha1-microglobulin/creatinine ratio was higher in both tested group of patients with UTI and fever. Those found in group A1 and B1 before treatment were the highest and statistically significantly elevated after treatment (group A2 and B2: p < 0.001). Our results indicate the usefulness of the urinary alpha-microglobulin/creatinine ratio as a marker of proximal kidney tubule damage in children with E. coli and Proteus sp. infections. Additionally, it seems to be associated with the humoral and cellular immune response.

Alpha-Globulins↗

PubMed2000

[Phagocytic activity of blood platelets before and after renal artery embolization in patients with renal cancer].

Embolization consists in the occlusion of renal artery and its ramifications, which induces acute renal infarction. Renal artery embolization, causing necrosis, stimulates cellular reactions within and around the neoplasm. The interaction of neoplastic cells with blood platelets is of great importance in the development of neoplastic disease. Neoplastic cells exert a stimulatory effect on blood platelets. During activation, platelets change in shape and secrete certain substances. We conducted this study measuring the phagocytic activity of blood platelets. 45 patients suffering from renal cancer were examined (22 women and 23 men) before and after embolization. Diagnosis was established basing on the patients' history, clinical picture, and ultrasonographic, urographic and angiographic examinations. The study demonstrated that embolization led to an increase in platelet count and decrease of phagocytic index.

Adult↗

PubMed2000

Flow cytometric analysis of CD 62P expression in patients with acute myocardial infarction.

Much attention has been paid to the role of platelets in the pathogenesis of heart diseases, e.g. ischaemic disease and myocardial infarction. During platelet activation changes appear both in the internal structure and in the membrane. The release reaction is associated with the appearance of CD 62P (P-selectin). The aim of the present study was evaluate CD 62P expression in patients with acute myocardial infarction according to the disease duration and the type of treatment. We study 29 patients with acute myocardial infarction (MI). The patients were divided into two groups: group A--15 patients treated with heparin and aspirin and group B--14 patients treated with streptokinase, heparin and aspirin. The control group consisted of 21 healthy subjects. We indicated that P-selectin expression is influenced by infarction duration and type of treatment. Our study demonstrates that thrombolytic therapy inhibits platelet activation. Patients receiving streptokinase showed lower CD 62P expression than those treated only with heparin and aspirin, both before and after platelet activation with thrombin.

Aspirin↗

PubMed2000

Platelet factor 4 as a marker of platelet activation in patients with acute myocardial infarction.

The aim of the present study was evaluate dynamics of platelet factor 4, as a marker of platelet activation, in patients with acute myocardial infarction according to the disease duration and type of treatment. In the recent years much attention has been paid to the role of platelets in the pathogenesis of ischaemic disease and myocardial infarction. Rupture or splitting of atheroma and increased platelet activation are a direct cause of acute thrombotic process in coronary vessels. During platelet activation alpha granules release proteins, e.g. platelet factor 4 (PF 4). We investigated 29 patients with acute myocardial infarction (MI); the patients were divided into two groups: group A--15 patients treated with heparin and aspirin; group B--14 patients treated with streptokinase, heparin and aspirin. Control group (C) consisted of 21 healthy subjects. PF 4 concentration was determined on the 1st, 3rd, 5th, 8th, 11th day of MI using the immunoenzymatic method. Our results indicate that in the course of myocardial infarction there is a change in the platelet factor 4 and that thrombolytic therapy inhibits platelet activation.

Aspirin↗

PubMed1999

Morphological parameters of blood platelets before and after renal artery embolization in patients with renal cancer.

Embolization consists in the occlusion of renal artery and its ramifications, which induces acute renal infarction. Renal artery embolization, causing necrosis, stimulates cellular reactions within and around the neoplasm. The interaction of neoplastic cells with blood platelets is of great importance in the development of neoplastic disease. Neoplastic cells exert a stimulatory effect on blood platelets. During activation, platelets change in shape and secrete certain substances. The aim of the present study was to evaluate morphological parameters of blood platelets in renal cancer patients before and after renal artery embolization. 45 patients suffering from renal cancer were examined (22 women and 23 men) before and after embolization. Diagnosis was established basing on the patients' history, clinical picture, and ultrasonographic, urographic and angiographic examinations. Tumour advancement was defined as T2 and T3. The study has demonstrated that embolization leads to an increase in platelet count and in the percentage of "megathrombocytes". The changes observed in the study may indirectly testify to the involvement of the thrombopoietic system in the course of neoplastic disease.

Adult↗

PubMed1999

Phagocytic activity of platelets in patients with myocardial infarction.

The aim of our present study was to analyse the dynamics of changes in the phagocytic activity of platelets with myocardial infarction according to the treatment applied. Twenty patients with acute myocardial infarction and thirty healthy subjects were examined. Platelet count, phagocytic activity of platelets and the phagocytic index were determined. We observed reduced phagocytic activity of platelets in the first 24 hours of infarction, which may be due to exhaustion of most active platelets in a thrombus and microaggregates. A transitory increase in the phagocytosis activity of platelets observed on the third day of infarction may be caused by the release of large, more active platelets from splenic pool. On the eight day of infarction, the phagocytic activity of platelets became normalized in both experimental groups. Our study indicates that together with platelet activation the phagocytic activity gets reduced being a likely response to the thrombocytopenic factor. This can be confirmed by the increased number of platelets on the 8th day. Our results suggest a slightly attenuated phagocytic ability of platelets, irrespective of the treatment applied. The reduction seems to result from the effect of platelets exhaustion on the procoagulative activity in patients with myocardial infarction.

Adult↗

PubMed1998

[Phagocytosis and killing of Staphylococcus aureus by mouse granulocytes after interleukin-3 (IL-3) injection].

Interleukin-3 is a multipotential hematopoietic growth factor, which like other colony stimulating factors (CSFs) is effective "in vitro" stimulation of the mature cells function. It was found that IL-3 synergistically with GM-CSF and G-CSF stimulated the proliferation of the granulocytes. Therefore the purpose of this investigation was the evaluation "in vivo" of the influence of IL-3 on the phagocytosis, bactericidal activity, and enzyme activities of granulocytes. IL-3 was injected into mice subcutaneously during 5 days in dose 1 microgram/kg/d. The examination of the percent of cells phagocytizing bacteria (Staphylococcus aureus), NBT test and bactericidal activity, were performed every day and evident increase of the tested parameters was found. Additionally the enzyme activities in primary granules were measured and showed on increase of acid phosphatase and peroxidase activity.

Acid Phosphatase↗

PubMed1998

Embolization and serum lysozyme activity in renal cancer.

The aim of the study was to determine the effect of renal tumor embolization on nonspecific immunity by evaluating lysozyme activity and leucocytosis in 45 patients and 40 healthy people. Lysozyme activity was assessed in the non-diluted serum (A1) and in the tenfold diluted serum (B1) prior to embolization and after embolization (A2, B2) and in control group. Prior to embolization, lysozyme activity was lower in the experimental group (A1 and B1), compared to the control groups, the differences being statistically significant (p < 0.05). After embolization, the activity became normalized (A2), reaching the control value and even exceeding it (C) in the diluted serum (B2). Leucocytosis prior to embolization (L1) resembled that of control group, increasing slightly after embolization (L2). The differences observed in the changes in lysozyme activity and leucocytosis were statistically significant (p < 0.05). Our findings indicate an inhibitory effect of the neoplastic process on nonspecific immunity. Embolization causes ischemic necrosis of tumor and products of neoplastic tissue disintegration exert a stimulating effect on granulopoiesis, by increasing the turnover of neutrophilic granulocytes. Granulocytic-monocytic infiltrations in tumor stroma are the source of lysozyme, enhancing not only local but also systemic immunity, which is manifested in the increased lysozyme activity in blood serum.

Adult↗

PubMed1998

Estimation of platelet function state in the course of trichinella spiralis infection.

Platelets are part of body defence system, especially the antiparasite immunologic response. Platelets manifest their functions only after their activation. Thrombin activates platelets inducing change of their shape and causing secretion of certain substances. This study was designed to estimate blood platelets morphology as an indicator of their activation and effectiveness of antiparasite therapy. The study was conducted in group of 21 patients infected with T.spiralis before treatment (T1) and group of 19 patients after two weeks of treatment (T2). The diagnosis of trichinellosis was established on the bases of epidemiologic history, clinical picture and immunologic investigations. In the course of trichinellosis and after antiparasitic treatment increase of blood platelet count was observed. The decreased mean platelets volume observed in trichinellosis may be connected with their activation and releasing platelet factor 4 and beta-thromboglobulin. We suggest that T.spiralis activates platelets and the degree of their activation determines their morphologic parameters.

Adolescent↗

PubMed1998

The activity of neutrophil myeloperoxidase in patients with renal cancer before and after embolization.

The activity of granulocytic myeloperoxidase (MPO) was investigated in 45 patients with renal cancer before and 14 days after renal artery embolization in comparison with control group of 40 healthy people. The group examined showed lower MPO activity before embolization, as compared to controls. The differences being statistical insignificant (p > 0.05). After embolization the activity MPO was normalized, reaching the mean value of control group. The findings revealed reduced granulocytic MPO activity in patients with renal cancer.

Adult↗