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H Kirchhoff

Publications and source records attributed to H Kirchhoff.

At least 73 records · Page 4Linked to original sources
PubMed1985

Application of the indirect enzyme immunoassay for the detection of antibodies against Erysipelothrix rhusiopathiae.

Sera from swine and rats experimentally infected with Erysipelothrix rhusiopathiae and field sera from swine were investigated for antibodies against E. rhusiopathiae using the microtiter enzyme immunoassay (EIA) and, for comparison, the growth test (GT) and the agglutination test (AT). In principle there was a good correspondence between the results of EIA and those of the two other methods, but EIA and GT were more sensitive than AT. On the basis of the evaluation pattern of GT and AT on swine sera, EIA titers of 1/320 were considered as "chronic erysipelas titers". Compared with GT and AT, the EIA has some advantages: it is not influenced by contamination of the test sera, it takes only a few hours and using the microtiter system it is easy and economical to perform.

Agglutination Tests↗
PubMed1984

Antigenic cross-reactions between Mycoplasma arthritidis and rat tissues.

An antigenic relationship between Mycoplasma arthritidis and rat tissues could be demonstrated using the enzyme-immune assay and the agar gel double diffusion test. Cross-reactions were observed with joint homogenates as well as with muscle, lung, liver, kidney, spleen and brain tissue from rats. In addition, antibodies against joint homogenate were found in the sera of rats infected with M. arthritidis ISR 1 using the indirect hemagglutination test. The significance of common antigens between M. arthritidis and rat tissues for the pathogenesis of the M. arthritidis polyarthritis in rats is discussed.

Animals↗
PubMed1984

Isolation of a motile mycoplasma from fish.

For the first time a mycoplasma has been isolated from fish. The organism, designated strain 163K, was isolated on modified Hayflick medium under aerobic conditions at 25 degrees C from the gills of a tench (Tinca tinca L.). It showed the characteristic features of mycoplasmas. In addition it was flask-shaped with a distinct head-like structure and was able to attach to inert surfaces and living cells. The most interesting property of the organism was its ability to show fast gliding motion. Movement was only in the direction of the head-like structure and was not interrupted by resting periods.

Animals↗
PubMed1984

Flask-shaped mycoplasmas: properties and pathogenicity for man and animals.

In spite of the variations in cell form and cell size, some mycoplasmas show several consistent and peculiar structural features: a flask- or club-shaped cell form and a more or less defined terminal structure. Organisms with these features can be observed within the species Mycoplasma pneumoniae, M. genitalium, M. pulmonis, M. gallisepticum, M. alvi, M. sualvi and Mycoplasma sp. strain 163 K. Ultrastructural peculiarities of some flask-shaped mycoplasmas are a surface nap and a cytoskeleton. With the exception of M. alvi and M. sualvi, for which detailed investigations are lacking, the flask-shaped mycoplasmas differ from the other mycoplasmas by their gliding motility and adherence properties. All of the flask-shaped mycoplasmas ferment glucose, but there are differences in the other biochemical properties investigated and in the guanine + cytosine ratio. The question remains whether the flask shape, the adherence and the motility are associated with pathogenicity, since three mycoplasmas with these properties are established pathogens. No data are available at present on the pathogenicity of the remaining four species, but several criteria suggest that at least two of them may have an etiological role in disease.

Adhesiveness↗
PubMed1983

Studies of polyarthritis caused by Mycoplasma arthritidis in rats. I. Detection of the persisting Mycoplasma antigen by the enzyme immune assay (EIA) and conventional culture technique.

Investigations on Mycoplasma (M.) arthritidis polyarthritis of the rat produced by intravenous inoculation of M. arthritidis ISR 1 with special reference to the persistence of the inoculated mycoplasma antigen were performed in a total of 145 rats. The mycoplasmas were demonstrated by the conventional culture technique as well as by an enzyme immune assay (EIA). M. arthritidis was cultured from liver, kidney, spleen, and thymus up to 4 days, from trachea and uterus up to 4 weeks, and from lung, heart, brain, and lymph nodes up to 2 months after infection. It could be recovered from the joints in 70-80% of the animals up to 28 days and in the remaining 20-30% up to 200 days after infection. Using the EIA the mycoplasmal antigen could be demonstrated generally, also in the joints of infected rats which had no longer been positive by culture (10-20 weeks after inoculation). The investigations using EIA also showed a positive reaction between antiserum against M. arthritidis and joint homogenates of control rats, indicating the presence of common antigens to M. arthritidis and to joint tissues of the rat.

Animals↗
PubMed1983

Studies of polyarthritis caused by Mycoplasma arthritidis in rats. II. Serological investigation of rats experimentally infected with M. arthritidis ISR 1.

In sera of rats which developed severe polyarthritis after infection with 10(9) CFU of Mycoplasma arthritidis ISR 1, antibodies against M. arthritidis could be detected by the complement fixation test (CFT), enzyme immune assay (EIA), indirect hemagglutination (IHA), and metabolic inhibition test (MIT). Peak titers (CFT - 1:4096, EIA - 1:2560, IHA - 1:640 and MIT - 1:64) were reached between 2 and 12 weeks after infection. The antibodies persisted for at least 9 months, but started to decrease gradually 5 to 7 months after infection. No antibodies against M. arthritidis could be detected in the growth inhibition test. Immune complexes were found 4 days after infection. They decreased rapidly during the first two months after infection after which they increased again. At no time, rheumatoid factors could be detected. Rats which had recovered from the polyarthritis induced by intravenous injection of M. arthritidis ISR 1 were resistant to a subsequent infection.

Animals↗
PubMed1983

Studies of polyarthritis caused by mycoplasma arthritidis in rats. III. Histopathological findings.

Mycoplasma arthritidis induced polyarthritis in the rat is characterized histologically by four distinct phases. Firstly, two days after infection, early lesions such as vascular changes and alterations in the lining cell layer of synovium are observed. This is followed four days after inoculation by an acute exudative phase with the deposit of fibrin and infiltration by polymorphonuclear granulocytes together with first signs of joint destruction by pannus-like granulation tissue. From about the third week onwards, lymphocytes and plasma cells predominate in the subsynovium, indicating subacute inflammation. In the fourth phase which lasts until the end of the one year observation period, chronic arthritis with destruction of cartilage and bone, ankylosis, and chronic inflammation, sometimes with acute recurrences, were observed in about 25% of the joints. Both the marked tendency to pannus formation and the longevity of the arthritis, make this an interesting, experimental model of chronic polyarthritis, particularly as it has been induced by a peptidoglycan-free microorganism.

Animals↗
PubMed1982

Serological investigation of horse sera for antibodies against mycoplasmas and acholeplasmas.

Sera from horses with respiratory disease (RD) have been investigated using the complement fixation test, indirect hemagglutination test, enzyme immune assay, and the metabolic inhibition test, and sera from mares after abortion, using the complement fixation test, indirect hemagglutination test and enzyme immune assay, for antibodies against Mycoplasma equirhinis, M subdolum, M. equigenitalium, M. pulmonis, M. felis, Acholeplasma laidlawii, A. hippikon and A. equifetale. Antibodies were found against all mycoplasma and acholeplasma species tested, more often against acholeplasmas. The antibody pattern was quite similar for horses with RD and for mares after abortion. The results of the four serological tests performed showed only a limited correlation and the percentage of sera with antibodies detected by the four tests used differed widely.

Abortion, Veterinary↗
PubMed1982

Isolation of acholeplasmas from horse feces.

Acholeplasmas were detected in five of 96 feces samples from clinically normal horses. Three of the five strains isolated were identified as A. equifetale, one as A. hippikon, and one was serologically identical with the Acholeplasma strain 881.

Abortion, Spontaneous↗