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Biomedical subjects

H Kirchhoff

Publications and source records attributed to H Kirchhoff.

At least 91 records · Page 5Linked to original sources
PubMed1981

Pathogenicity of Spiroplasma sp. strain SMCA in Syrian hamsters: clinical, microbiological, and histological aspects.

The intracerebral inoculation of newborn Syrian hamsters with pure cultures of Spiroplasma sp. strain SMCA caused severe, prolonged disease involving the central nervous system, culminating in death. The disease was characterized by spasms, muscular tremors, disturbances in motor control, inability to feed, dramatic loss of weight, and runting. The effect ws dose related, with the largest numbers of viable spiroplasmas producing the highest incidence of disease and death in the shortest period of time. Severe hemorrhaging developed throughout the brain, liver, and spleen, and spiroplasmas were readily recovered from these organs, indicating that the agent disseminated from the initial site of infection to distant host tissues. Newborn animals were susceptible, but adults were resistant; these findings are similar to those reported for newborn mice and rats. Unlike mice and rats, hamsters did not develop cataracts visible to the unaided eye. The histopathological features of eye disease in hamsters were different from those in rats and were characterized by microophthalmia (especially in runted hamsters) and abnormal proliferation, disorientation, and disorganization of corneal, lens, and retinal tissues. The significance of these findings is discussed.

Animals↗
PubMed1981

Pathogenicity of Spiroplasma sp. strain SMCA in rabbits: clinical, microbiological, and histological aspects.

Newborn rabbits inoculated intracerebrally with early-passaged broth cultures of Spiroplasma strain SMCA (suckling mouse cataract agent) either died or developed eye disease. Death occurred 4 to 12 days after infection. Rabbits which died showed hemorrhages throughout the brain, especially in areas leading to the optic nerve, and hemorrhages in the liver. SMCA could be reisolated from brain, liver, and eyes. Rabbits with eye disease did not open their eyes during an observation time of 3 to 4 months. Pathological examination revealed marked microophthalmia and severe cataract formation characterized by complete degeneration of lens fibers and liquefaction and mineralization of the lens. Other ocular changes were chronic panophthalmitis with complete destruction of the retina. Neither eye disease nor death could be induced by inoculating SMCA subcutaneously into newborn rabbits. In adult rabbits, no disease occurred after intravenous or subcutaneous injection or after inoculation into the conjunctival sac.

Aging↗
PubMed1980

[Mycoplasmas isolated from the genital tract of mares (author's transl)].

Mycoplasmas were isolated from 11 (=#6,8%) of 161 cervix swabs from infertile mares. A total of 17 strains was isolated and characterized by indirect immunofluorescent test and metabolic inhibition test as Mycoplasma equigenitalium (11 strains), Mycoplasma subdolum (2 strains), Acholeplasma laidlawii (3 strains) and Acholeplasma equifetale (1 strain). In addition cervix swabs of the mares were investigated for bacteria. There was no specific correlation between presence of mycoplasmas and bacteria (table 1). In clinical investigations 5 of the 11 mares which harboured mycoplasmas showed a pneumovagina (table 1). The isolated mycoplasmas were tested for senstivity to antibiotics (table 2). All of the isolates were sensitive to Chloramphenicol (10 microgram), Tetracyclin (10 microgram), Tylosin (30 microgram) and Gentamycin (10 microgram) and resistant to Penicillin (6 IU) and Polmyxin B (300 IU). Against Erythromycin (15 microgram) all of the Acholeplasma-isolates were sensitive and all of the Mycoplasma-isolates resistant. Against Streptomycin (10 microgram) the two M. subdolum strains showed resistance, whereas M. equigenitalium and the Acholeplasma-isolates were sensitive. It is not yet possible to elucidate the significance of acholeplasmas and mycoplasmas in the genital organs of mares.

Acholeplasma↗
PubMed1980

[Investigation of mare sera for antibodies against acholeplasmas and mycoplasmas with ther enzyme linked immunosorbent assay (ELISA) (author's transl)].

After abortion sera were taken from 58 thoroughbred and other mares of the northwestern part of Germany and investigated by ELISA (enzyme linked immuno-sorbent assay) for antibodies against Mycoplasma equirhinis, M. subdolum, M. equigenitalium, M. pulmonis, M. felis, Acholeplasma laidlawii, A. hippikon, and A. equifetale. Reactions at serum dilutions of 1:32 and higher were considered as positive. At serum dilution 1:32 no antibodies were found in 11 sera. The remaining sera showed antibodies against one or more of the mycoplasma antigens investigated. The number of multiple reactions decreased with an increasing dilution of the sera. Titers were found between 1:32 and 1:256. In one case a titer of 1:2048 against M. equigenitalium antigen was found. Most often antibodies against A. laidlawii were observed i.e. in 37 sera. These antibodies also showed the highest titers. Only 3 sera contained antibodies against A. hippikon. Antibodies against M. felis and A. equifetale were found in 26 sera. Between 10 and 15 sera showed antibodies against the remaining mycoplasma species.

Abortion, Veterinary↗