[Studies on the sensitivity of Mycoplasma hyorhinis strains against various antibiotics and against nitrofurazone].
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Biomedical subjects
Publications and source records attributed to H Kirchhoff.
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Breech delivery is still attended by considerably higher perinatal mortality and morbidity than is the normal presentation. Additional diagnostic methods are, therefore, needed for the safe conduct of breech deliveries. Roentgenograms in the lateral projection are a simple, but too rarely used, means that, in conjunction with the clinical findings, will provide essential information regarding the obstetric technique to be adopted. The objections to "X-ray diagnosis during pregnancy" are totally unfounded it Guthmann's single exposure technique is used. A retrospective analysis of 221 roentgenograms of breech presentation was correlated with the course of delivery. The results were highly informative in respect of both the pelvic abnormality and, especially, the therapeutic consequences. The X-ray evidence made it possible to reduce perinatal mortality (uncorrected) to 1.8 percent (generally stated to be 10--15 percent) and corrected mortality to nil.
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Mycoplasms isolated from heart valves (A. laidlawii (A 42 Hzbtl)) and pericardium (M. canis (A 56 Hzkl)) of dogs were investigated for infectivity of rats, mice and gerbils (Meriones unguiculatus). In mice the species M. spumans (PG 13), M. maculosum (PG 15), M. edwardii (PG 24) and M. molare (H 542) were investigated too. The mycoplasmas were inoculated by oral, subcutaneous, intravenous and intraperitoneal route. Intraperitoneally the mycoplasmas were given without and with complete and incomplete Freund adjuvants. The injected Mycoplasmas did not produce any disease and could not be reisolated from rats and gerbils. From mice A. laidlawii (A 42 Hzbtl), M. canis (A 56 Hzkl) and M. edwardii (PG 24) could be reisolated after intraperitoneal administration in emulsion with complete and incomplete Freund adjuvants. Whereas M. canis (A 56 Hzkl) and M. edwardii (PG 24) could be reisolated from lung, liver and spleen only, A. laidlawii (A 42 Hzbtl) was present in heart muscle too. In histological investigations the mice which got A. laidlawii (A 42 Hzbtl) intraperitoneally with complete Freund adjuvants showed focal lymphocytic infiltrations in the myocardium.
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Proteins extracted with phenol-acetic acid-water (2:1:0.5, w/v/v) from Acholeplasma laidlawii (PG 8), A. granularum (BTS-39), A. oculi (19L), A. modicum (PG 49), A. axanthum (S743) and the Acholeplasma strains C1 and C112 (which were isolated from aborted horse foetuses) were compared by electrophoresis in horizontal acidic polyacylamide flat gel using the electrophoresis equipment LKB Multiphor 2117. In this system the gels are not prepared in the electrophoresis chamber but between glas plates. For electrophoresis they are applied onto a special cooling plate. This makes it possible to produce a number of identical gels (from the same gel mixture and polymerized under the same conditions) what can be important for comparing investigations. The gels can be stored for more than 4 weeks in the refrigerator at +4 degrees C. Marked differences were observed between the electrophoretic patterns of each of the established species and the horse strains C1 and C112. The results are in agreement with those obtained in serological investigations in which the strains C1 and C112 were different from the established Acholeplasma species.
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The modern examination methods for the recognition and supervision of high risk births in the area of perinatology have led to a real decrease in newborn morbity and mortality. Since, however, optimal conditions have not been reached at present, there is an urgent need for a wide reaching intensification of prospective diagnostic measures. The early recognition of disturbances in the mechanism of labor is the most important. The author shows on the basis of the most recent literature and his own experience that, even today, factors involved with the mechanics of labor play a chief role in disturbances during the course of labor which have unfavorable effects on the infant as well as the mother. The various irregularities were discussed in detail and supported with statistics. The importance of pelvic roentgenographic diagnosis was discussed in a separate section. The tremendous gains for mother and infant resulting from clarification were compared with the cited, but not confirmed, damage caused by these measures.
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