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Biomedical subjects

H M Khalil

Publications and source records attributed to H M Khalil.

At least 37 records · Page 2Linked to original sources
PubMed1990

Specificity of crude and purified Fasciola antigens in immunodiagnosis of human fascioliasis.

A study has been done to find the effect of purification of crude antigens extracted from adult F. gigantica and F. hepatica on the cross reactions encountered in C.I.E.P., I.H.A. and E.L.I.S.A. serological techniques with sera o other parasitic and non-parasitic diseases. It was performed on 75 patients and 20 healthy controls. It was found that sera of many diseases may cross-react with crude Fasciola antigen in serological diagnosis. These diseases include schistosomiasis, hydatidosis, amoebic liver abscess, heterophyiasis, trichinosis, non parasitic liver diseases (liver neoplasms, pyogenic liver abscess, viral hepatitis and acute leukaemic) and rheumatoid arthritis. Partial purification of crude Fasciola antigens is a suitable method to avoid cross reactivity when using C.I.E.P. or to diminish them when using E.L.I.S.A. No need for this purification when using I.H.A. So using partially purified adult Fasciola antigens C.I.E.P. was the most specific test (100%) of followed by C.L.I.S.A. then I.H.A.

Animals↗
PubMed1990

Specific immunoglobulins in schistosomiasis.

Specific and total immunoglobulins antibodies levels to Schistosoma antigens were determined in 44 patients with S. mansoni infection. No Significant increase was observed in parasitic specific IgG, IgM, and IgA levels in both acute and chronic infections. On the other hand, Significant increased specific IgE antibodies was demonstrated in both acute and chronic infections of bilharziasis. The results revealed that increased immunoglobulins was not Specific to Schistosomiasis infection. Also, No correlation was observed between clinical course of the infection and immunoglobulins levels.

Animals↗
PubMed1990

Immunodiagnostics of Toxocara canis in suspected ocular and visceral manifestations.

Sera from patients with ocular and visceral manifestations of toxocariasis were tested by precipitin absorption (PAT), counter-immunoelectrophoresis (CIEP), indirect fluorescent antibody (IFAT) and ELISA. The ocular cases revealed a percentage of positivity of 36% by PAT, 0% by CIEP, 32% and 28% by IFAT with embryonated egg (EE) and frozen section antigens (FS) respectively and 40% by ELISA. The visceral cases revealed 44% positively by PAT, 24% by CIEP 52% and 48% by IFAT with EE and FS respectively and 52% by ELISA. Statistical evaluation was done to interpret the results.

Adult↗
PubMed1990

Evaluation of some direct diagnostic methods in intestinal parasitic infections.

Stool specimens of 100 cases attending the outpatient clinic of Cairo Medical Center Hospital suffering from gastro-intestinal disturbances were examined for intestinal parasites by direct smear, trichrome stained smear and formol ethyl acetate concentration methods. Although formol ethyl acetate concentration method gave the best results (45%), followed by trichrome stain (37%) then direct smear (32%) yet, for a reliable diagnosis a combination of several techniques is required.

Evaluation Studies as Topic↗
PubMed1989

The relation between the two forms of COPT and levels of total IgG and IgM in acute and chronic schistosomiasis mansoni.

COPT was performed on 55 acute and 48 chronic male patients with schistosomiasis mansoni as well as on 20 control normal subject. The percentage of the segmented and the bleb reactions were determined. The total levels of IgG, IgM and IgA were measured. There was no distinct evidence that COPT shows only bleb precipitations in acute cases and only segmented reactions in chronic cases. The total levels of IgG were higher in patients who showed segmented that in those who showed bleb reactions especially in the segmented but not the bleb reactions especially in chronic cases. IgM may be responsible for the bleb reactions in acute cases.

Animals↗
PubMed1989

The immunodiagnosis of Schistosoma mansoni infection in the different clinico-parasitological stages.

A total of 52 bilharzial mansoni patients were examined, 20 patients with early intestinal infection with living ova in stools, 20 patients with hepatosplenomegaly but without ascites, also with viable ova in stools, and 12 patients with hepatosplenomegaly and ascites without ova in stools; but in the rectal snip. Also 20 normal persons were examined. An IDT test was performed by S. mansoni adult worm antigen. S. mansoni adult worm and egg antigen extracts were used for performing CIEP, IHAT and ELISA tests showed statistically the same high sensitivity. In cases with hepatosplenomegaly whatever the clinical (with or without ascites) or the parasitological status (passing or not passing ova in stools) of the patients, the ELISA test with egg antigen is found to be a highly sensitive and a specific test.

Animals↗
PubMed1989

The application of S. mansoni adult and soluble egg antigens for the serodiagnosis of schistosomiasis by CIEP, IHA and ELISA.

The sero-reactivity of 82 schistosomiasis infected patients were studied by the use of the CIEP, IHA and ELISA tests using S. mansoni adult worm antigen (AWA) and soluble egg antigen (SEA). CIEP with (AWA) showed a progressive sensitivity decreased with advance of S. mansoni infection, and increased in sensitivity by (SEA). The test was more sensitive in the early cases of S. haematobium infection by (AWA), while by (SEA) it was more sensitive in the late cases. There was no difference in sensitivity of the test with either antigens in mixed bilharzial infection. IHAT with (AWA) showed high sensitivity in the different stages of S. mansoni infection, but sensitivity decreased with the progress of clinical status of S. mansoni if (SEA) was utilized. The test with (AWA) was slightly more sensitive in the early cases of S. haematobium than the late cases, while the same high sensitivity was given by the test with (SEA) in both groups. Also IHAT with (AWA) was more sensitive for the diagnosis of mixed bilharzial infection than with (SEA). ELISA test with (SEA) showed a progressive increase in sensitivity with the advance of S. mansoni. Also the test with (SEA) was more sensitive than with (AWA); and for the diagnosis of cases of mixed bilharzial infection, while S. haematobium infected cases showed a lower sensitivity than S. mansoni or mixed bilharzial infection cases, if the ELISA test is performed with either antigens.

Animals↗
PubMed1989

Immunodiagnostics of visceral toxocariasis.

Twenty five suspected cases of visceral toxocariasis and 25 apparently healthy controls, were tested in four serological tests, namely precipitin absorption test (PAT), counter immunoelectrophoresis (CIEP), indirect fluorescent antibody test (IFAT), and enzyme linked immunosorbent assay (ELISA). Adult Toxocara worm antigen was used in PAT & CIEP as saline extract and as frozen sections (FS) in IFAT while the embryonated eggs (EE) were used as saline extract in ELISA and as a whole in IFAT. The percentages of positivity reported were 44% by PAT, 24% with CIEP, 52% with IFAT (EE), 48% with IFAT (FS), and 52% in the ELISA. In the apparently healthy control group, only one subject gave positive result with PAT, and ELISA at titres of 1/16 and 1/64 respectively. The EE antigen proved to be more sensitive than adult worm antigen, where it was used in IFAT & ELISA and gave the highest percentage of positivity and the highest titre (1/256 & 1/4096 respectively). It was noticed that the children below the age of 10 years, were statistically significant (P less than 0.05) by all tests except CIEP.

Adolescent↗
PubMed1989

Sensitivity of crude and purified Fasciola antigens in immunological diagnosis of human fascioliasis.

A study has been done to find the effect of purification of crude antigens, extracted from adult Fasciola gigantica and Fasciola hepatica worms on the sensitivity of some serological techniques in diagnosis of fascioliasis. Using C.I.E.P. test, the partially purified antigens of both Fasciola species were less sensitive than their corresponding crude ones. Using I.H.A., the difference between results of crude and partially purified antigens for the two species were statistically insignificant, both (P greater than 0.05). So in applying this test, there is no need for the partially purification of antigen. On using E.L.I.S.A. the partially purified antigens showed higher sensitivities for detection of fascioliasis than their corresponding crude ones. Partially purified F. gigantica and F. hepatica antigens gave nearly the same results whether using C.I.E.P., I.H.A. or E.L.I.S.A. However, E.L.I.S.A. was the most sensitive technique followed by I.H.A. then C.I.E.P.

Animals↗
PubMed1989

Relationship between schistosomiasis and arthropathy.

This study was carried out to suggest criteria for diagnosing arthritis associated with schistosomiasis. 180 cases were classified into three clinical groups, 120 schistosomal arthritic group (I), 20 schistosomal non-arthritic (II), 20 arthritic non-schistosomal (III) and 20 controls (IV). Four tests were done to exclude other causes of arthritis namely, Erythrocyte sedimentation rate (ESR), Rose-Waaler test (RW) for rheumatoid factor (RF), antinuclear antibody test (ANA) and determination of serum uric acid (SUA) level. A history, clinical examination, urine and stool examination, intradermal test (IDT), indirect haemagglutination test (IHAT), circumoval precipitin test (COPT) and complete blood picture, were performed for all groups. 20 patients were selected randomly from group I and received praziquantel to be followed up 6 months later. Bilharzial ova were found in the excreta of group I and II. The percentage of positive IDT, IHAT and COPT in I & II was 90.8%, 90%, 88.3% and 85% respectively. Blood picture showed mild anaemia and from low to moderate eosinophilia. ESR was moderately raised. RF positivity was 6.6%, 10%, 65% and 5% in the four groups respectively. ANA positivity was 1.6%, 0%, 50% and 0% respectively. There was a marked improvement of arthritic manifestations after praziquantel in 90% of cases.

Arthritis, Infectious↗