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Biomedical subjects

H Pusch

Publications and source records attributed to H Pusch.

At least 37 records · Page 2Linked to original sources
PubMed1986

[Pregnancy anamnesis and delivery in females with abnormalities of the internal genitalia].

The ability to deliver a living infant was examined in 65 women with genital malformations, in a retrospective clinical study. 87 deliveries resulted, the overall abortion rate was 35%. 44% of pregnancies proceeded without any complications, in 46% the first pregnancy led to the birth of a living child. Classification of genital malformations displayed a dominancy of severe forms like uterus duplex, bicornis and septus. Comparison of severe and mild uterovaginal anomalies revealed no significant differences concerning course and duration of pregnancy, birth weight and perinatal outcome of the newborn, whereas frequency of operative deliveries raised significantly in the group of severe defects, Apgar scores after one minute appeared significantly lower. A remarkable accumulation of breech presentations (50%) in combination with increased obstetric complications led to 82% of operative deliveries. The birth of 58% of boys caused a shifting of the sex ratio. The perinatal offspring was encouraging; 73% of the newborn could leave the clinic together with their mothers.

Abortion, Habitual↗
PubMed1986

[Status of the childless couple].

Data of 150 couples were evaluated in a retrospective study at the Infertility Outpatient Clinic of the Department of Gynaecology and Obstetrics of the University of Graz. Investigations were based on a standardised andrological schedule of inquiry, consisting of 163 items. 23 of them concerned the couple itself, 111 items referred to the man, 29 to the woman. Obtaining general data on the situation of the couple was a special aim of the study. The age of the patients, duration of marriage and the desire to have children, contraception and sexual habits were assessed. Furthermore, special andrological problems, such as previous diseases of the genital organs, results of palpation and spermatograms, were points of interest. Anamnestic data on the evaluation of the female partner have also been an important subject of investigation in the present study. The average duration of an infertile marriage was 5.1 +/- 3.3 years, the average time of desire of children was 3.8 +/- 2.7 years. 36% of the couples had practised contraception for a mean time of 1.0 +/- 1.7 years. 33% of the men had suffered from a disease of the genital organs. Pathological palpatory findings occurred in 49%. Only 10% of the patients had a normal spermiogram. In 21% of women an attempt had been made to evaluate sterility via invasive diagnostic methods without sufficient evaluation of fertility in the male partner. An overall pregnancy rate of 24.7% was achieved.

Adult↗
PubMed1985

Serum magnesium in insulin-dependent diabetics and healthy subjects in relation to insulin secretion and glycemia during glucose-glucagon test.

UNLABELLED: In order to investigate the influence of insulin secretion on serum magnesium concentrations 33 insulin-dependent diabetics and 10 control subjects were studied. The residual insulin secretion (RIS) was investigated by measurement of human C-peptide (HCP) before and after stimulation during on OGTT (1.75 mg/kg)-glucagon (i.v. 0.1 mg glucagon/kg)-test. RESULTS: Certain RIS existed in 11 insulin-dependent diabetics, 12 were without any RIS (uncertain RIS in 10 patients). Glucose tolerance and daily glycemia differed significantly among the two groups. However, all diabetics were far from euglycemia, (3.3-9.3 mmol/l): Fasting plasma glucose 12.0 +/- 0.9 (certain RIS), 15.0 +/- 0.8 (no RIS), 13.9 +/- 1.8 (uncertain RIS). Serum magnesium was significantly lower in all diabetics, both before and during the test. There was no change during the OGTT-glucagon-test and no difference among the three groups of insulin-dependent diabetics. So, we conclude that a small RIS in our longterm insulin-dependent diabetics has no influence on the behaviour of serum magnesium. But, magnesium depletion can influence coronary blood flow, blood clotting, and atherogenesis. Therefore, it should be necessary to pay more attention to the hypomagnesemia in insulin-dependent diabetics.

Adult↗
PubMed1985

[Fetomaternal blood transfusion as a cause of severe obstetrical complications].

A Caesarean section was performed in a healthy secundipara of 26 years of age in the 39th week of gestation, because of severe alterations of foetal heart-rate patterns, signalling foetal distress. Amount and colour of the amniotic fluid appeared normal. A highly anaemic male infant was delivered (BW 2920 g, BL: 49.5 cm, RBC: 770 000/mm3, PVC: 11%). Since perinatal posttraumatic bleeding was excluded by the normal appearance of the umbilical cord and the placenta, transplacental foeto-maternal macrotransfusion was suggested immediately and diagnosis confirmed by demonstrating foetal red cells in the maternal circulation (79%) using a modified Kleihauer technique. Other causes of anaemia in the newborn such as haemolysis, coagulopathies and failure of red cell production were excluded later. Foetal erythrocytes can be demonstrated in the maternal circulation up to 80 days after delivery, provided the blood groups of mother and foetus are compatible. If this is not the case, there will be a rapid elimination of foetal red cells. Hence, it appears advisable to conduct a Kleihauer test directly if foeto-maternal transfusion is suspected. It may well be that some of the cases of unexplained foetal death are due to this phenomenon.

Adult↗
PubMed1984

On the temperature dependence of the Na pump in sheep Purkinje fibres.

The temperature dependence of cardiac active Na transport is studied in voltage clamped sheep Purkinje fibres by means of simultaneous measurements of the membrane current (I) and the intracellular Na activity (alpha iNa). During activation of the Na pump a transient outward current (delta I) and alpha iNa decline exponentially with an identical time constant (tau). The transient outward current and the decline in alpha iNa are blocked by 10(-4) M dihydroouabain (DHO). Lowering the temperature from 42 degrees C to 17 degrees C prolongs tau. The electrogenic fraction (e.f.) of the active Na efflux remains unaffected. The Q10 value of the active Na transport derived from the changes of tau varies within the temperature range studied. The Q10 amounts to approximately 1.2 between 42 degrees C and 35 degrees C, to approximately 2.4 between 35 degrees C and 22 degrees C and to approximately 2.1 between 35 degrees C and 17 degrees C. Correspondingly the activation energy of the active Na transport is not constant between 42 degrees C and 17 degrees C. It is calculated to be 3.4 kcal/mol between 42 degrees C and 35 degrees C, 15.9 kcal/mol between 35 degrees C and 22 degrees C and 12.4 kcal/mol between 35 degrees C and 17 degrees C. Variations in temperature change the maximal rate constant of the active Na transport, whereas the sensitivity of the Na pump towards the extracellular K concentration (Ko) is little affected.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1983

[Liquefaction disorders of the ejaculate--diagnosis and therapy].

We are introducing a simple and reliable method for evaluation of liquefaction disturbances of human ejaculate. This technique requires to try to draw threads out of the ejaculate by using plastic hootes. If this can still be achieved after a period of 20 minutes after ejaculation, liquefaction is positively disturbed. The therapeutical possibilities have been investigated in a clinical study. 37 patients of the andrological department of the university hospital for gynecology and obstetrics in Graz have been treated with Ichthyol-medicaments for six weeks. 18 patients had a sit-bath therapy with Ichthobad three times a week, 19 patients were using Ichtopur suppositoria two times daily. In both variants of treatment a significant decrease of the liquefaction time occurred, 3 pregnancies resulted. This new diagnostic method and the encouraging results after treatment with Ichthyol improve the therapeutical possibilities for the andrologist in the complex field of seminal liquefaction disturbances.

Adult↗
PubMed1983

[Psychosocial background in sterility patients].

150 childless couples from the infertility clinic of the department of gynecology and obstetrics, University of Graz, received a questionnaire. Psychosocial factors and special problems of childless marriage represented the background at which the investigation was aimed. Of special interest were the reactions of the male partner regarding the andrological investigation within the gynecological department. The patients were confronted by a total of 41 questions. 15 of them were equal for both partners, whereas the male partner had to answer 11 additional questions. The questionnaire concerned problems such as interactions of the couple, motivations for the desire of children, psychosomatics, andrological investigation within the gynecological department and the organization of the andrological outpatient section. 72% of the questionnaires were returned. More than 50% of the sterile couples preferred to attend the infertility clinic together. 26% felt restrictions in their sexual behaviour due to the unrealized desire of children, 48% expected an improvement in their partnership if they could have children. 98% considered their childless marriage as a problem of both partners. The desire to set up a family ranks first on the list of motivations. The evaluation of the questionnaire yielded valuable results for the infertility clinic and especially for the andrological department. 72% of the andrological patients did not feel embarrassed by being evaluated and treated in a women's department. 63% preferred to attend the andrological department jointly with their wife.

Female↗
PubMed1982

An identification of the K activated Na pump current in sheep Purkinje fibres.

The cell membrane of sheep Purkinje fibers hyperpolarizes transiently on returning to K containing media after several minutes in K free solution. To analyse this 'K activated response' voltage clamp experiments and measurements of the internal Na activity (aiNa) are performed in fibres bathed in solution containing 0.2-2 mM BaCl2. Compared to the response in Tyrode solution the transient hyperpolarization beyond the resting potential is increased in Ba containing media. The response is blocked by 10(-4) M dihydroouabain. During the response, in Ba treated fibres, aiNa and a transient outward current decline with the same time constant at all clamp potentials tested. The transient outward current is most probably due to a temporary increase in electrogenic Na pumping. Both the amplitude and the time constant of the pump current show little voltage dependence. The electrogenic fraction of the active Na efflux is estimated to be about 39% and is independent of aiNa. Ba ions facilitate the analysis of the pump current in voltage clamped fibres because K depletion is reduced and changes of the I-V relationship by K depletion are minimized. It is concluded that activation of the electrogenic Na pump is mainly responsible for the K activated response of fibres in Ba containing media.

Animals↗
PubMed1981

Activation of active Na transport in sheep Purkinje fibres by external K or Rb ions.

The intracellular Na activity (aiNa) of sheep Purkinje fibres bathed in solutions with and without K (Rb) is studied by means of Na sensitive microelectrodes. Perfusion with K (Pb) free media increases aiNa. Upon reapplication of K (Pb) containing solutions aiNa decreases and cell membrane hyperpolarizes transiently. After an initial delay attributed to K (Rb) equilibration in the extracellular space, the decline of aiNa and membrane potential towards their respective resting values is approximately monoexponential and displays the same time constant (tau). The tau values and aiNa in the steady state vary with the extracellular K (Rb) concentration ([K]0, [Rb]0). According to a simple model the activation of the Na pump by external K (Rb) can be estimated from the time constants or the aiNa steady state values at various [K]0 ([Rb]0). Half maximal activation occurs at 1.6-3.7 mM K (Rb). External K and Rb ions are equipotent activators of the Na pump in sheep Purkinje fibres.

Animals↗
PubMed1979

A kinetic model for the muscarinic action of acetylcholine.

The timecourse of the membrane hyperpolarization evoked by stimulation of postganglionic parasympathetic nerve endings in isolated atria from the guinea-pig heart is mainly governed by two exponentials, one describing most of the rising phase (rate constant k alpha = 2.88+/-1.135 s-1) and one which completely describes the decline of the response (k beta = 0.58+/-0.31 s-1). An exact description of the muscarinic receptor mediated potential change, which also allows for its apparent latency and its s-shaped beginning, is found if two additional faster exponentials are introduced. In agreement with earlier results a model of four consecutive reactions is presented. It is concluded that during muscarinic cholinergic transmission reactions subsequent to binding of the ACh-molecules to the receptor are rate-limiting.

Acetylcholine↗
PubMed1976

Effects of Na and K ions on the active Na transport in guinea-pig auricles.

1. The effect of Na and K ions on active Na transport was studied in guinea-pig auricles by means of flame photometry. 2. The Na influx into preparations rewarmed in Tyrode's solution after cooling was estimated to be about 1.05 mmole/l fibre water - min (l.f.w.-min) or c. 8 pmole/cm2 - s. Intracellular Na ions enhanced the active Na efflux over a wide range of concentrations. A decrease in the extracellular Na concentration ([Na]o) had no major effect on the active Na efflux. 3. Extracellular K ions initiated an active Na efflux from rewarmed auricles with an elevated [Na[i over a narrow range of K concentrations ([K]o). 4. Assuming Michaelis-Menten kinetics the maximal active Na efflux activated by internal Na ions was calculated to be about 4 mmole/l.f.w. - min (30 pmole/cm2 - s). Half maximal Na efflux occurred at about 22 mmole/l.f.w. [Na]i. The maximal K-activated active - min (28 pmole/cm2 - s) and was half maximal at a [K]o of about 0.2 mM. 5. It is tentatively concluded that the maximal active Na efflux from guinea-pig atria is 3--4 times larger than the physiological flux. Under normal conditions active Na efflux in heart is mainly regulated by variations of [Na]i.

Animals↗