PubMed Health⌕ Search

Biomedical subjects

H Rahman

Publications and source records attributed to H Rahman.

At least 19 recordsLinked to original sources
PubMed2006

Gross morphometry of human placenta in eclampsia.

The study was done to see the gross morphologic changes of placenta in human normal pregnancy and eclapmsia, with the aim that it would be able to increase the general body of knowledge regarding the gross morphology of normal placenta and the changes that occur in eclampsia in our population. Total of 45 (n=45) placenta, 25 (n=25) from eclampsia and 20 (n=20) from normal pregnant cases were collected from Gynaecology and Obstetric Department of Mymensingh Medical College & Hospital (MMCH). Study was done in Anatomy Department of Mymensingh Medical College (MMC). Macroscopic study of the formol saline fixed placentas revealed that, compared to the controls there was trends of lower weights and volumes of placentas in eclamptic group. Statistical significance of difference between two groups was calculated by using Students "t" test. A difference between the two groups was considered to be significant when p<0.005.

Adolescent↗
PubMed2006

A morphological and morphometric study of the right ventricular papillary muscles of autopsied heart of Bangladeshi people.

Fifty formalin-fixed hearts from thirty nine male and eleven female Bangladeshis aged 20 to 70 years and without any known cardiac disorder were used in the present study. In right ventricle, there was a single anterior papillary muscle in 92% cases. The posterior papillary muscle was double in 32% and single in 28% cases. The septal papillary muscle was single in 46% cases while in 30% cases it was absent. The different between males and females in the total number of papillary muscles was statistically non significant. In the right ventricle, the anterior papillary muscles were the longest and the septal papillary muscles were the shortest.

Adult↗
PubMed2006

Morphological study of recurrent laryngeal nerve in relation to thyroid gland.

The study was done to see the relationship of recurrent laryngeal nerves with corresponding inferior thyroid artery with the aim to increase the knowledge regarding variational anatomy in our population. Sixty samples were collected as block dissection of thyroid gland along with the surrounding structures containing the supplying vessels and adjacent nerves from 48 male and 12 female cadavers of different age groups and fixed in 10% formol-saline solution. Fine dissections were carried out to study the toporgaphic relationship of recurrent laryngeal nerves with corresponding inferior thyroid artery. The findings were compared between different age groups and also with the findings of western researchers. In this study, in majority of the cases the recurrent laryngeal nerves were found in their usual location that is posterior to the terminal branches of inferior thyroid artery on the left side and anterior to that artery on the right side. It was important to note that, on both sides recurrent laryngeal nerves were also frequently found to pass in between the terminal branches of inferior thyroid artery.

Adolescent↗
PubMed2006

Prevalence & phenotypic expression of sopB gene among clinical isolates of Salmonella enterica.

BACKGROUND & OBJECTIVE: Salmonella induced enteritis is a complex phenomenon involving a number of pathogenic factors. Type III secretions play a central role in the development of Salmonella induced enteritis. One such Type III secretion protein is Salmonella outer proteinB (SopB). Reports on the prevalence of this gene among different serovars of Salmonella of Indian origin appears to be lacking. The present investigation reports on the prevalence of sopB gene and its phenotypic expression (SopB protein) among different serovars of Salmonella enterica isolated from man and animals. METHODS: A total of 50 isolates of S. enterica belonging to 11 serovars isolated from cases of enteric infection in man, birds and animals were tested for the presence of sopB gene by polymerase chain reaction (PCR) using its specific primers. The in vitro phenotypic expression of SopB protein was detected by dot-ELISA using anti-SopB serum. RESULTS: All the 50 isolates of S. enterica belonging to 11 were found to carry sopB gene irrespective of their serovars like Typhimurium, Enteritidis, Gallinarum, Choleraesuis, Virchow, etc., and source of isolation. Of these, 41 isolates were found to express sopB gene phenotypically as detected by dot- ELISA using anti-SopB serum. Of the different serovars, all but Gallinarum expressed Sop B protein phenotypically in vitro. INTERPRETATION & CONCLUSION: Our findings indicated that sopB gene was wildely distributed and conserved among Salmonella irrespective of their serovars and source of isolation. Further work need to be done to study the factors associated with the phenotypic expression of this gene.

Animals↗
PubMed2005

Seroprevalence of brucellosis in mithuns (Bos frontalis) in India.

Studies conducted on the seroprevalence of brucellosis in 98 mithuns maintained at the National Research Centre on Mithun, Nagaland, India revealed that the number of animals found positive for brucellosis in avidin-biotin enzyme-linked-immunosorbent assay, standard tube-agglutination test and Rose-Bengal plate test were 34, 20 and 11%, respectively. Highest prevalence of brucellosis was observed in the Mizoram mithun strain. The relative sensitivity and specificity of the standard tube-agglutination test were 61 and 100%, respectively; the corresponding values of the Rose-Bengal plate test were 33 and 100%, respectively.

Animals↗
PubMed2005

Occurrence of sopE gene and its phenotypic expression among different serovars of Salmonella enterica isolated from man and animals.

Salmonella pathogenesis is a complex phenomenon and a Type III secretion system plays a central role in the development of Salmonella-induced enteritis. One such Type III secretion protein is Salmonella outer protein E (SopE). Prevalence of sopE gene and its phenotypic expression (SopE protein) among different serovars of Salmonella enterica isolated from man and animals were investigated. Of 305 strains of S. enterica belonging to 11 serovars tested for the presence of sopE, 130 strains belonging to three serovars viz., Enteritidis, Gallinarum and Virchow were found to carry sopE gene irrespective of their source of isolation when tested by PCR amplification technique using its specific primers. Of these 130 strains, 112 strains were found to express SopE protein phenotypically as detected by Dot-ELISA using SopE antibody. Among the different serovars tested only serovars Gallinarum, Enteritidis and Virchow expressed SopE protein phenotypically in vitro. Role of SopE protein in pathogenesis of salmonellosis has been discussed.

Animals↗
PubMed2005

Morphometric study of atrioventricular orifices of postmortem hearts of adult Bangladeshi people.

To study the circumference of atrioventricular orifices of the Bangladeshi adult people, fifty postmortem hearts were collected, fixed in 10% formol saline and examined. In the present paper, the findings were compared between male and female values and also with the findings of Western and Bangladeshi researchers. Among fifty hearts, thirty-six hearts were collected from male and fourteen hearts were collected from female cadavers. The findings suggest that the atrioventricular orifices of the hearts examined were lower in circumferences as compared to those described in the available texts. The female values were slightly lower than the male values but on statistical analyses, they showed no significant difference (P > 0.05).

Adult↗
PubMed2005

Isolation, phage typing and antibiogram of Salmonella from man and animals in northeastern India.

BACKGROUND AND OBJECTIVE: Salmonella is an important zoonotic pathogen and its prevalence in the animals acts as a continuous threat to man. The present study was carried out to report the isolation along with the serotypes, phage types and antibiogram pattern of Salmonella among man, livestock and poultry in the northeastern India. METHODS: A total of 654 samples from diarrhoeic livestock and humans were processed for the isolation of Salmonella. All the isolates were subjected to antibiogram studies against 15 antimicrobials. Representative isolates of S. Typhimurium and S. Enteritidis were phage typed. RESULTS: Ninety five isolates of Salmonella enterica belonging to 5 serotypes- S. Typhimurium, S. Enteritidis, S. Gallinarum, S. Paratyphi B and S. Bareilly were obtained with an overall prevalence rate of 14.40 per cent. S. Typhimurium isolates were distributed among four phages- DT003, DT004, DT096 and DT193 and all the S. Enteritidis isolates belonged to a single phage type, PT13a/7. Interspecies sharing of the phages was observed. Norfloxacin, enrofloxacin, gentamycin and ciprofloxacin were most effective, whereas, doxycycline, ampicillin, amoxycillin and tetracycline were relatively less effective. INTERPRETATION AND CONCLUSION: Our findings showed that three of the five serovars as well as some of the phage types of these serovars were shared by animals and humans indicating the zoonotic potential of the organism. Thus, it is imperative that salmonellosis control measures adopted for humans should give adequate importance to its control in the animals particularly their products.

Animals↗
PubMed2005

Delayed post-operative wound infections due to non-tuberculous Mycobacterium.

BACKGROUND & OBJECTIVE: The non-tuberculous mycobacteria (NTM) have emerged as important opportunistic pathogens of human beings in the recent years. The NTM are rapid growing mycobacteria (RGM), which include Mycobacterium fortuitum and M. chelonae and grouped as M. fortuitum-chelonae complex. Though there are reports on isolation of NTM from various parts of India, information on its occurrence in northeastern India is lacking. We therefore undertook this preliminary investigation to report on the occurrence of NTM-associated with non-healing postoperative wound infections that did not respond to antibiotics used for pyogenic infections and having sterile routine aerobic cultures in patients from northeastern part of India. METHODS: Pus/discharge from 25 patients with delayed onset of post-operative wound infections not responding to antibiotics used for pyogenic infections were collected and examined for isolation and identification of the causative agents. RESULTS: Of the 25 pus/discharge specimens examined, 20 revealed growth of non-tuberculous Mycobacterium spp. All the isolates were identified as M. fortuitum-chelonae complex. Of these only 10 samples revealed acid-fast bacilli (AFB) on direct examination of Ziehl-Neelsen stained smears from the specimens. All cases where direct smear was positive for AFB were also positive for Mycobacterium culture. INTERPRETATION & CONCLUSION: The results of the present study indicated that non-tuberculous mycobacterial post-operative wound infection was fairly common in northeastern India. Thus, mycobacterial infections should be considered in wounds that show delayed healing and do not respond to antibiotics used for acute pyogenic infections. Further, 80 per cent of the specimens yielded the growth of AFB in cultures as against only 40 per cent positive in the ZN stained direct smears. This indicates the possibility of missing a mycobacterial wound infection if only direct smears are taken for diagnosis.

Adult↗
PubMed2005

Salmonellosis in pigmy hogs (Sus salvanius)--a critically endangered species of mammal.

The pigmy hog (Sus salvanius) is the smallest and the rarest wild suid in the world. This species is on the verge of extinction and the World Conservation Union has rated it among the most endangered of all mammals. This paper reports the investigation into an outbreak of salmonellosis among captive pigmy hogs at the Research and Breeding Centre of the pigmy hog conservation programme, Guwahati, Assam, India. Of 75 pigmy hogs (38 males and 37 females) maintained at the Centre, seven (9.3%) died within five days. The causative organism associated with the outbreak was identified as Salmonella Typhimurium (syn. S. enterica serovar Typhimurium). All the isolates of S. Typhimurium belonged to phage type DT193. The isolates harboured multiple plasmids. Five isolates harboured four (65.0 MDa, 4.2 MDa, 3.0 MDa, 1.3 MDa), while two isolates carried three plasmids (65.0 MDa, 4.2 MDa, 3.0 MDa). All strains showed resistance to amikacin, ampicillin, streptomycin and sulfamerazin; five strains were resistant to oxytetracycline and trimethoprim. All the strains were sensitive to chloramphenicol, ciprofloxacin, enrofloxacin and gentamicin. All seven isolates of S. Typhimurium were found to harbour stn, sopB and pefA genes. However, none of them was found to carry sefC and sopE genes.

Animals↗
PubMed2005

Effect rHuEpo on predialysis CRF patients: study of 45 cases.

Forty five (24 male & 21 female) moderate to severe degree of predialysis CRF patients were prospectively studied over a period of 6 months (July- December, 2004) to see the effect of Recombinant Human Erythropoietin (rHuEpo/EPO) therapy on renal anaemia, progression of renal excretory function & quality of life at 3 and 6 months intervals from the starting of EPO therapy. Mean +/- SD age of the patients was 56 +/- 12 (30-77 yrs) and causes of CRF were Diabetic Nephropathy (DN)=15 (33%), Chronic Glomerulonephritis (CGN) =14(31%), Hypertension (HTN)=11(21%), Chronic Pyelonephritis (CPN)=03 (6.5%) and Obstructive Uropathy (OU)=02 (4.5%). Doses of rHuEpo was 80-100 IU/k week subcutaneously (SC) until the target Hb 11gm% & Hct 30% were achieved; there after the dose was titrated as appropriate. Serum Iron & Ferritin levels were also kept within normal reference level by iron therapy during the study period. Mean +/- SD base line (before starting EPO therapy) level of haemoblobin were 8.4 +/- 0.81(gm%), Hct 27.86 +/- 1.6 (%), blood urea 21.72 +/- 10.5 (mmol/L), S. creatinine 431.93 +/- 228.79 (mmol/L) & Ccr. 21.25 +/- 10 mum respectively. The results showed that significant improvement of haemoglobin level occurred (gm%) from 8.4 +/- 0.81 (gm%) to 9.51 +/- 1.02 (p<0.001) at 3 months and 8.4 +/- 0.81 to 11.10 +/- 1.4, (p<0.001) at 6 months interval. Haematocrit (Hct%) value also significantly increased from 27.86 +/- 1.5 to 30.57 +/- 3.62, (p<0.001) at 3 months and 27.86 +/- 1.5 to 32.81 +/- 3.92 (p<0.001) at 6 months of EPO therapy. Mean blood urea and S. creatinine levels decreased from base line level during the study period but did not show any statistical significance. There was no significant side-effects like uncontrolled hypertension, seizure or hyperviscosity syndrome in any of the study population. The quality of life in terms of improvement of physical ability and sense of well being were also improved in all the study patients. In conclusion, this study showed that the effect of rHuEpo therapy is beneficial for the correction of renal anaemia, can delay the progression of renal failure and improvement of overall quality of life in predialysis CRF patients.

Adult↗
PubMed2004

Seroprevalence of infectious bovine rhinotracheitis in mithun (Bos frontalis) in India.

A preliminary study on the seroprevalence of infectious bovine rhinotracheitis (IBR) in different strains of mithun maintained atthe National Research Centre on Mithun, Nagaland, India, revealed that the overall prevalence of IBR in these mithun was 19%. This paper examines the results of that preliminary study. To the knowledge of the authors, this is the first report on the seroprevalence of IBR in mithun in India. The highest prevalence of IBR was observed in mithun found in Arunachal Pradesh (38.46%), followed by those found in Mizoram (18.18%) and Nagaland (15.15%). None of the animals from Manipur were found to test positive for antibodies against IBR. The sex of the animal had no influence on IBR prevalence. The prevalence was found to be highest (27.03%) in mithun above three years of age and lowest (7.69%) in mithun aged between six months and one year. The prevalence of IBR was found to be 88.9% in breeding mithun bulls. In this paper, the authors briefly discuss the possible roles of feral fauna and domestic livestock in the transmission of this disease to mithun and vice versa. Various measures that may help in the prevention and control of IBR in mithun are also surveyed. However, it should be emphasised that this study is a preliminary one, and the authors are currently engaged in further research.

Age Factors↗
PubMed2004

Presence of sopE gene & its phenotypic expression among different serovars of Salmonella isolated from man & animals.

BACKGROUND & OBJECTIVES: Salmonellae cause a spectrum of diseases in man and animals but their virulence factors responsible for induction of gastroenteritis and/or systematic infection are still poorly understood. Also, the different subspecies and serovars of Salmonella differ considerably in their virulence for man and animals. There is increasing evidence that Salmonella possesses a dedicated protein secretion system denoted type III secretion system (TTSS) that is involved in the early stage of Salmonella infection. One such TTSS is Salmonella outer protein E (SopE) that helps in the invasion of Salmonella by stimulating membrane ruffling. In the present study the presence of sopE gene and its phenotypic expression (SopE protein) among different serovars of Salmonella enterica isolated from man and animals in India was investigated. METHODS: A total of 50 isolates of S. enterica belonging to 11 serovars were tested for the presence of sopE gene by polymerase chain reaction. The in vitro phenotypic expression of SopE protein was detected by Western blotting using anti-SopE serum. RESULTS: Of the 50 isolates of S. enterica belonging to 11 serovars tested for the presence of sopE,14 belonging to three serovars viz., Enteritidis, Gallinarum and Virchow were found to carry the sopE gene. Similarly, 13 isolates belonging to same three serovars were found to express SopE protein phenotypically as detected by Western blotting using anti-SopE serum. INTERPRETATION & CONCLUSION: The results indicated that sopE gene appeared to be distributed and conserved among only a few serovars of Salmonella (Enteritidis, Gallinarum and Virchow) irrespective of their source of isolation. The presence of sopE gene in Salmonella provides an important pathogenic means to invade epithelial cells.

Animals↗
PubMed2003

Distribution of virulence genes in Salmonella serovars isolated from man & animals.

BACKGROUND & OBJECTIVES: Pathogenesis of Salmonellosis depends upon a large number of factors controlled by an array of genes that synergise into the actual virulence of Salmonella. A study was undertaken to observe the distribution of three such genes, namely, Salmonella enterotoxin (stn), Salmonella Enteritidis fimbrial (sef and plasmid encoded fimbrial (pef genes, among different serovars of Salmonella enterica isolated from man and animals. METHODS: A total of 95 isolates belonging to S. Typhimurium (51), S. Enteritidis (36), S. Bareilly (3), and S. Paratyphi B (5) serovars were subjected to polymerase chain reaction (PCR) assay for the detection of stnl ssf and pef genes using their specific primers and the PCR products were analysed by 1 per cent agarose gel electrophoresis for the presence of the respective genes. RESULTS: Varying distribution pattern of these genes was observed amongst the isolates. While, stn was found in all the 95 strains, sef was found only among the S. Enteritidis isolates. The pef gene was found to be absent in 10 isolates including the three S. Bareilly isolates. INTERPRETATION & CONCLUSION: Findings indicated that the stn gene is widely distributed among Salmonella irrespective of the serovars and source of isolation. However, the sef gene appears to be serovar specific. Since the stn gene is found in all the isolates, it can be a viable target gene to explore the possibility of direct detection of Salmonella from samples from biological sources.

Adhesins, Bacterial↗
PubMed2002

Microsatellite DNA and RAPD fingerprinting, identification and genetic relationships of hybrid poplar (Populus x canadensis) cultivars.

Microsatellite DNA markers of ten SSR loci and 248 RAPD loci (resolved by 26 RAPD primers) were used for DNA fingerprinting and differentiation of 17 widely grown Populus x canadensis syn. Populus x euramericana (interspecific Populus deltoides x Populus nigra hybrids) cultivars ("Baden 431", "Blanc du Poitou", "Canada Blanc", "Dorskamp 925", "Eugenei", "Gelrica", "Grandis", "Heidemij", "I-55/56", "I-132/56", "I-214", "Jacometti", "Ostia", "Regenerata", "Robusta", "Steckby" and "Zurich 03/3"), and determination of their genetic interrelationships. Informativeness of microsatellite and RAPD markers was also evaluated in comparison with allozyme markers for clone/cultivar identification in P. x canadensis. High microsatellite DNA and RAPD genetic diversity was observed in the sampled cultivars. All of the 17 P. x canadensis cultivars could be differentiated by their multilocus genotypes at four SSR loci, and were heterozygous for their parental species-specific alleles at the PTR6 SSR locus. Except for "Canada Blanc" and "Ostia", which had identical RAPD patterns, all cultivars could also be differentiated by RAPD fingerprints produced by each of the two RAPD primers, OPA07 and OPB15. For microsatellites, the mean number of alleles, polymorphic information content, observed heterozygosity, observed number of genotypes and the number of cultivars with unique genotypes per locus was 5.2, 0.64, 0.67, 5.7 and 2.2, respectively. For RAPD markers, the number of haplotypes per locus, and the number of cultivars with unique RAPD profiles per locus were 1.06 and 0.72, respectively. Overall, microsatellite DNA markers were the most informative for DNA fingerprinting of P. x canadensis cultivars. On the per locus basis, microsatellites were about six-times more informative than RAPD markers and about nine-times more informative than allozyme markers. However, on the per primer basis, RAPD markers were more informative. The UPGMA cluster plots separated the 17 cultivars into two major groups based on their microsatellite genotypic similarities, and into three major groups based on their RAPD fragment similarities. Both the microsatellite and RAPD data suggest that the cultivars "Baden 431", "Heidemij", "Robusta" and "Steckby" are genetically closely related. The inter-cultivar genetic relationships from microsatellite DNA and RAPD markers were consistent with those observed from allozyme markers, and were in general agreement with their speculated origin. Microsatellite DNA and RAPD markers could be used for clone and cultivar identification, varietal control and registration, and stock handling in P. x canadensis.

Alleles↗
PubMed2002

Some aspects of molecular epidemiology & characterisation of Salmonella Typhimurium isolated from man & animals.

BACKGROUND & OBJECTIVES: Salmonellae are widely distributed in nature and cause a wide spectrum of diseases in man and animals. Among the different serovars of Salmonella enterica, S. Typhimurium is most commonly associated with enteric infection in man and animals. The present paper reports on the phage typing, plasmid profile, antimicrobial resistance and genetic diversity of Indian strains of S. Typhimurium isolated from man and animals. METHODS: A total of 17 strains of S. Typhimurium were subjected to phage typing and tested for plasmid and antimicrobial resistance. Genetic variability of the strains was studied by cleaving the whole cell DNA with restriction endonuclease, XbaI and detecting the restriction fragments by pulse-field gel electrophoresis (PFGE). RESULTS: All the 17 strains were typeable and belonged to 4 phage types (DT003, DT004, DT096 and DT193). Three plasmid profiles were observed. Multiresistance was common. Strains belonged to DT193 were highly resistant to most of the antimicrobial agents tested and harboured 3-4 plasmids. Strains belonged to DT003, DT004 and DT096 were found to be less resistant to antibiotics and harboured only 1 plasmid. A genetic variability was observed among the strains irrespective of the source of isolation. Twelve restriction patterns observed among the 17 strains as analyzed by PFGE indicated a considerable genetic heterogeneity among human and animal isolates. INTERPRETATION & CONCLUSION: A relationship was observed between resistance patterns, plasmid profiles and phage typing. Restriction analysis obtained by PFGE was found to be more discriminating in the differentiation of strains than the other methods used, and thus provides a valuable alternative tool for molecular characterisation of S. Typhimurium isolated from man and animals.

Animals↗