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Biomedical subjects

H Sano

Publications and source records attributed to H Sano.

At least 649 records · Page 36Linked to original sources
PubMed1984

Identification of sulfur-containing metabolites of m-dichlorobenzene and their disposition and relationship with glutathione in rats.

The sulfur-containing metabolites of m-dichlorobenzene (m-DCB) were identified by using gas chromatography-mass spectrometry and disposition of these metabolites was studied. In the blood, urine and feces of rats dosed with m-DCB, 2,4- and 3,5-dichlorophenyl methyl sulfoxides and 3,5- and 2,4-dichlorophenyl methyl sulfones emerged, while their possible precursors, 3,5- and 2,4-dichlorophenyl methyl sulfides (Me' and Mf', respectively), were not detected in the blood, urine and feces. However, after heating the alkalinized urine and feces, the methyl sulfides appeared. Each cumulative amount of the methyl sulfoxides and methyl sulfones excreted in both the urine and feces for 120 h was less than 0.3% of the dose, while the amounts of Me' and Mf' excreted were about 0.9 and 0.2%, respectively. The amounts of 2,4- and 3,5-dichlorophenyl mercapturic acids excreted in the urine were 2.3 and 1.2%, respectively. However, the former was increased to 27.3% and the latter to 4.3% after heating the acidified urine. The cystein conjugates were not detected. The administration of m-DCB decreased the glutathione level in liver of fasted rats but it was not further reduced by diethyl maleate (DEM)-pretreatment. The kidney glutathione level was not altered by the administration of m-DCB. The blood level of methyl sulfoxides and methyl sulfones and their excretion in urine were markedly decreased in DEM-pretreated rats, while the mercapturic acids in acid-treated urine of DEM-pretreated rats were not significantly decreased. These results suggest that the methyl sulfoxides and methyl sulfones were derived from glutathione in liver and the formation process of mercapturic acids was somewhat what different from that of methyl sulfoxides and methyl sulfones.

Acetylcysteine↗
PubMed1984

Role of surgery in hypertensive intracerebral hematoma. A comparative study of 305 nonsurgical and 154 surgical cases.

The indications for surgery in hypertensive intracerebral hematoma are still controversial. The reason for this may be: 1) lack of adequate and comparable data in conservative and surgical therapy from the same institution; 2) lack of adequate close follow-up monitoring over an extended period of time; or 3) lack of proper classification of hematomas for comparison of results from different institutions. The authors have treated 459 cases of hypertensive intracerebral hematoma between October, 1975, and July, 1983. The hematomas have been classified according to their mode of extension on computerized tomography. The long-term outcome was assessed on the basis of activity of daily living. Putaminal hematomas were classified as mild, moderate, severe, and very severe. In general, there was no significant difference in outcome between the surgical and nonsurgical cases; however, the outcome in the moderate and severe hematomas was found to be a little better for the surgical cases in some restricted areas. Thalamic and pontine hemorrhages were classified as mild, moderate, or severe. If the hematoma is localized to the thalamus or pons, and if it extends to the midbrain, there is no indication for surgery; however, in patients with moderate hematomas, the prognosis showed a variable outcome, and the indications for surgery were questionable. In cerebellar hematomas, the authors propose that even a hematoma with a diameter greater than 3 cm might show a good outcome with nonsurgical therapy.

Activities of Daily Living↗
PubMed1984

Chemical modification of spiramycins. III. Synthesis and antibacterial activities of 4''-sulfonates and 4''-alkylethers of spiramycin I.

Among the derivatives protected with t-butyldimethylsilylether of spiramycin I, 2'-O-acetylspiramycin I 3,18-(O-t-butyldimethylsilyl)acetal was found to be a suitable intermediate for 4''-modification of spiramycin I. Seven 4''-sulfonates and four 4''-alkylethers were synthesized, which were more active against bacteria in vitro than spiramycin I. 4''-Substituted derivatives with relatively small sulfonyl and alkyl groups were comparable in therapeutic effect to spiramycin I.

Animals↗
PubMed1984

Chemical modification of spiramycins. IV. Synthesis and in vitro and in vivo activities of 3'',4''-diacylates and 3,3'',4''-triacylates of spriamycin I.

3'',4''-Diacylates and 3,3'',4''-triacylates of spiramycin I were synthesized and evaluated by the four parameters, MIC against bacteria, affinity to ribosomes, retention time in HPLC and therapeutic effect. Among them, 3,3'',4''-tri-O-propionyl and 3,4''-di-O-acetyl-3''-O-butyryl-spiramycin I were the most active in vivo, which were superior to acetylspiramycin.

Animals↗
PubMed1984

[Indication of minimized regional operation for primary liver cancer].

In the cirrhotic patient with inadequate liver functional reserve, curative standard resection due to anatomic basis often lead to liver failure postoperatively. Therefore, prognosis and resectability remained unsatisfactory for these patients. Definition of minimized regional operation which is performed to the patient with decreased functional reserve of the liver is not clearly determined. In our department, definition of minimized regional operation is made as follows. 1. The liver is impaired to more than moderate degree. (Child B and C or decreased liver functional reserve) 2. Maximum tumor diameter is less than 5 cm. 3. The extent of resection is less than subsegmentectomy. Study of 23 patients undergoing this minimized regional operation was made and the result is as follows. In relation of extent of resection to tumor diameter, in patients with less than 3 cm tumor recurrence developed only in one (7.6%) of thirteen patients undergoing minimized regional operation and none of four undergoing conventional standard resection. While in patients with more than 3 cm tumor, recurrence was found in two (25%) of eight patients with minimized regional operation and three (60%) of five patients with resection of one segment. In relation of the extent of resection to tumor capsule, in patients with less than 3 cm tumor with capsule, minimized regional operation and three standard resections were performed and in none of which recurrence developed. In patients with less than 3 cm tumor without capsule, recurrence developed in one of four patients undergoing minimized regional operation. While in patients with more than 3 cm tumor, recurrence developed in two (25%) of eight patients with tumor possessing capsule and three (60%) of five patients without capsule. As a result, in patients with less than 3 cm tumor diameter, minimized regional operation is acceptable with little recurrence but in patients with more than 3 cm tumor with or without capsule, anatomic lobe resection is necessary and resection of more than two segments is mandatory if functional reserve of the liver allows. In our department, extent of resection is determined by the functional reserve of the liver which is graded into three degree. For subsegmentectomy, following laboratory date has to be met: ICG-Rmax greater than 0.4, sigma IRI/sigma BS greater than 0.2, Ch-E greater than 500 IU/1, Total Bil. less than 2.5 mg/dl, S-Alb greater than 3.0 g/dl, GPT less than 300 IU and HPT greater than 50%.(ABSTRACT TRUNCATED AT 400 WORDS)

Chronic Disease↗
PubMed1984

Nuclear DNA degradation in lymphocytes of patients with systemic lupus erythematosus.

The degradation of nuclear DNA in lymphocytes of patients with systemic lupus erythematosus (SLE) was analyzed. A low m.w. DNA fragment was identified in phytohemagglutinin (PHA)-stimulated lymphocytes from both healthy controls and SLE patients. The rate of appearance of low m.w. DNA was at least fourfold higher in lymphocytes from SLE patients, suggesting that DNA from SLE cells are more easily degraded than that from normal cells. PHA-stimulated lymphocytes from SLE patients were also shown to be more sensitive to UV irradiation than those of controls. These results suggest that DNA metabolism is abnormal in T cells of SLE patients.

Adult↗
PubMed1983

Characterization of DNA methyltransferase from bovine thymus cells.

A DNA methyltransferase was partially purified from bovine thymus heavy cells. The enzyme has Mr 130 000, and introduces methyl groups from S-adenosylmethionine into the 5 position of cytosines in DNA. Sequence specificity analysis revealed that about 60% of the total methylation occurred in the 5'd(C-G)3' doublet. Single-stranded and hemi-methylated DNAs were methylated at an elevated rate by the enzyme. The kinetic analysis showed that the reaction obeys a random sequential mechanism. These results suggest that the enzyme serves primarily as a maintenance DNA methyltransferase.

Animals↗
PubMed1983

Turnover and oxidation rates of blood glucose and heat production in sheep exposed to heat.

Five shorn sheep were used to study effects of heat exposure (30 degrees C, relative humidity 70%) on metabolism of blood glucose and heat production. The isotope dilution method was applied for determination of metabolism of blood glucose in a thermoneutral environment (20 degrees C, relative humidity 70%) and on the 4th and 10th days of heat exposure. Concentrations of free fatty acids and thyroxine of plasma decreased during heat exposure with no reduction in feed intake. At 20 degrees C, glucose turnover rate, percentage of glucose oxidized, and contribution of exhaled carbon dioxide derived from glucose to the total carbon dioxide production were 6.2 mg/kg.75 per min, 34%, and 11%. Glucose turnover rate decreased significantly during heat exposure, but the proportion of glucose oxidized was unchanged. Heat production decreased almost parallel to the glucose turnover rate during heat exposure, resulting in an unchanged contribution of carbon dioxide derived from glucose to the total production of carbon dioxide. Blood glucose metabolism and heat production of sheep decrease, but the contribution of blood glucose to heat production remains unchanged during exposure to 30 degrees C.

Animals↗
PubMed1983

[Experimental and clinical evaluation of cefpiramide in pediatrics].

Fundamental and clinical studies on cefpiramide (CPM), a new semisynthetic cephalosporin, were made and the following results were obtained. The antibacterial activities of CPM against clinical isolates were almost similar to those of conventional cephems except for Pseudomonas aeruginosa. The antibacterial activity of CPM against P. aeruginosa was excellent and superior than those of the others. Ten or twenty mg/kg of CPM was given intravenously at one shot to 11 cases. The mean serum levels of CPM reached 231 micrograms/ml at 15 minutes, 119 micrograms/ml at 30 minutes, 88 micrograms/ml at 1 hour, 65 micrograms/ml at 2 hours and 33 micrograms/ml at 6 hours after administration at a single dose of 10 mg/kg, respectively with the half-life of 3.42 hours. In case of 20 mg/kg, the mean serum levels attained 306 micrograms/ml at 15 minutes, 245 micrograms/ml at 30 minutes, 160 micrograms/ml at 1 hour, 118 micrograms/ml at 2 hours and 66 micrograms/ml at 6 hours respectively after administration with the half-life of 5.20 hours. CPM was given intravenously to 12 patients with various bacterial infections. The clinical effects were excellent in 5 cases, good in 6 cases and poor in 1 case and the effective rate was 92%. No side effect was observed in all cases.

Adolescent↗