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Biomedical subjects

H Seiler

Publications and source records attributed to H Seiler.

At least 19 recordsLinked to original sources
PubMed2005

Posttraumatic anterior-inferior instability of the shoulder: arthroscopic findings and clinical correlations.

INTRODUCTION: The main purpose of our study was to evaluate intra-articular lesions in glenohumeral-instability with arthroscopy and correlate them with clinical findings as well as history of instability. MATERIAL AND METHODS: In this prospective multi-centre study, we evaluated arthroscopic findings in 303 patients with posttraumatic anterior-inferior instability of the shoulder. The study cohort was divided into 2 groups: patients with a history of one dislocation (Group 1, n=61, 20.1%) and patients with a history of more than one dislocation (Group 2, n=242, 79.9%). RESULTS: In Group 1, 37 patients had an IGHL-lesion, 31 a MGHL-lesion and 41 a Hill-Sachs lesion. In Group 2, 182 patients had an IGHL-lesion, 172 a MGHL-lesion and 203 a Hill-Sachs lesion. The percentage of lesions in Group 2 (IGHL-75.2%, MGHL-71.1%, Hill-Sachs-83.9%) was significantly higher than in Group 1 (IGHL-60.7%, MGHL-50.8%, Hill-Sachs-67.2%, P=0.0233, P=0.0026, and P=0.0033, respectively). Within Group 2 we found significantly more Hill-Sachs-lesions with a history of an increasing number of recurrences (P=0.0436). We also found an increase of IGHL- and MGHL-lesions with an increasing number of recurrences, but this difference was not significant. The distribution of lesion types of the anterior labrum-ligament complex showed no significant difference between the two groups, apart from a higher incidence of ALPSA-lesions within Group 2 (34.7% versus 18.0% in Group 1). The results of this study show that recurrences after primary posttraumatic anterior-inferior shoulder dislocation cause increasing ligamental damage as well as increasing Hill-Sachs lesions within the gleno-humeral joint. CONCLUSION: Thus we conclude that early surgical stabilization after posttraumatic anterior-inferior shoulder dislocation is necessary to prevent increasing damage within the shoulder joint.

Adolescent↗
PubMed2005

Allergic sensitisation and allergic rhinitis are associated with n-3 polyunsaturated fatty acids in the diet and in red blood cell membranes.

OBJECTIVE: Due to inconsistent results based on dietary intake data, unsaturated fatty acids in red blood cell (RBC) membranes and diet were used to investigate their association with allergic sensitisation and allergic rhinitis. DESIGN: Cross-sectional, population-based study. SETTING: Bavarian Nutrition Survey II (2002-03), Germany. SUBJECTS: A total of 568 adult participants, 325 women and 243 men. METHODS: By means of logistic regression models, the relation of fatty acids to (i) allergic sensitisation as defined by means of specific serum immunoglobulin E analysis (CAPSX1 class > or = 2), and (ii) self-reported allergic rhinitis was examined. RESULTS: A high cell membrane level of eicosapentaenoic acid (EPA, 20:5 n-3) was inversely associated with allergic sensitisation, the adjusted odds ratio (OR) and 95% confidence interval (95% CI) were 0.52 (0.30-0.90) for the highest (vs lowest) quartile. A similar effect was observed for allergic rhinitis with an OR (95% CI) of 0.50 (0.24-1.03; P = 0.027 for trend). A higher dietary intake of alpha-linolenic acid (ALA, 18:3 n-3) was associated with a decreased risk of allergic sensitisation and allergic rhinitis with ORs (95% CIs) of 0.51 (0.28-0.93) and 0.43 (0.20-0.93), respectively, in the highest quartiles. No other dietary or cell membrane unsaturated fatty acid was significantly associated with the outcome variables, nor was the n-6/n-3 ratio. The strongest effects were observed among subjects under the age of 40 y. CONCLUSIONS: In this cross-sectional study among adults, a high content of n-3 fatty acids in RBC membranes (EPA) or in the diet (ALA) is associated with a decreased risk of allergic sensitisation and allergic rhinitis.

Adult↗
PubMed2000

Polyphasic identification of wild yeast strains isolated from Greek sourdoughs.

A total of forty-five wild yeast strains were isolated from five traditional Greek wheat sourdoughs. Strains were identified using the classical identification technique along with the sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole cell proteins (SDS-PAGE), Fourier transform-infrared spectroscopy (FT-IR) and the randomly amplified polymorphic DNA-polymerase chain reaction analysis (RAPD-PCR). The latter methods confirmed the classical identification. According to the results obtained, fourteen strains were identified as Saccharomyces cerevisiae strains, twenty-five as Pichia membranaefaciens strains and six as Yarrowia lipolytica.

Bread↗
PubMed2000

Quantification of micro-organisms in binary mixed populations by Fourier transform infrared (FT-IR) spectroscopy.

Fourier Transform Infrared (FT-IR) spectroscopy was used for the first time to determine the ratios of different microorganisms in mixtures. Exemplarily, systems composed of two food-associated yeast species (Saccharomyces cerevisiae/Hanseniaspora uvarum) and two yoghurt lactic acid bacteria (Lactobacillus acidophilus/Streptococcus salivarius ssp. thermophilus) were investigated. Determination of the cell number ratio in the lactic acid bacteria system was possible with a minimal prediction accuracy of +/- 16 ratio percentage points while the minimum accuracy of prediction in the yeast two-component system was +/- 4% (both at a 95% confidence level). These results show that FT-IR spectroscopy is potentially a rapid method for the quantification of cell ratios in mixtures of two different microorganisms, provided that the cell ratio does not drop below a certain, system-specific threshold.

Bacteria↗
PubMed1999

[The upper ankle joint. Biomechanics and functional anatomy].

Dorsoplantar motion in the upper ankle joint is around one "transverse" rotational axis. In relation to the lower leg this axis is oblique joining the tips of the malleoli. Talocrural motion is highly precise compared to other human joints. Most of the stability and undisturbed motion in the loaded joint is afforded by the intimate osteocartilagineous contact. The rule of the collateral ligaments is secondary stabilisation, buffering of abnormal stresses and centering the talus in the extremes of motion. The posterior talofibular ligament is the universal lateral stabilizer, the deep portion of the deltoid ligament is the predominant medial ligament structure. Ligament tension without strain and under valgusabduction load as a rule makes only a difference in a quantitative way. Under varusadduction-stress load patterns are usually changed. Fibula and the distal syndesmotic ligaments, in the presence of valgusabduction- and external rotation stress, predominate over the medial joints structures. Remaining joint steps, especially in Volkmanns triangle (posterior malleolus) are prearthritic deformities. Nevertheless primary traumatic lesion of the cartilage has a major prognostic meaning. In cadaver joints as in vivo permanent lesions of only a singular fasciculus of the deltoid or lateral collateral ligament result in clearly detectable motion abnormalities. Concerning clinical therapy at present as in the past complete reconstruction of all that damaged singular structures must be claimed for. There is generally only a small tolerance against instability and malposition in the upper ankle joint. Even after one hundred years of research today factors defining the individual breadth of tolerance are not fully understood. In the presence of treatment regimes, that stood the test of time, it is therefore difficult to recommend treatment alternatives, relaying on individual biomechanical tolerance.

Ankle Injuries↗
PubMed1998

Rapid and reliable identification of food-borne yeasts by Fourier-transform infrared spectroscopy.

Computer-based Fourier-transform infrared spectroscopy (FT-IR) was used to identify food-borne, predominantly fermentative yeasts. Dried yeast suspensions provided the films suitable for FT-IR measurement. Informative windows in the spectrum were selected and combined to achieve optimal results. A reference spectrum library was assembled, based on 332 defined yeast strains from international yeast collections and our own isolates. All strains were identified with conventional methods using physiological and morphological characteristics. In order to assess identification quality, another 722 unknown yeast isolates not included in the reference spectrum library were identified both by classical methods and by comparison of their FT-IR spectra with those of the reference spectrum library. Ninety-seven and one-half percent of these isolates were identified correctly by FT-IR. Easy handling, rapid identification within 24 h when starting from a single colony, and a high differentiation capacity thus render FT-IR technology clearly superior to other routine methods for the identification of yeasts.

Cluster Analysis↗
PubMed1997

[Spinal stabilization in extradural metastatic disease. Indications and follow-up of 32 cases].

Over 4.5 years, 32 patients with spinal epidural metastases were decompressed and stabilized. Median survival was 9.5 months. Myelopathy was the predominant indication (41%) for the operation, intractable pain (microinstability) the second most important. The type of tumor spreading and biomechanics necessitated ventral decompression and stabilization in 65%. Corporectomy or extensive laminectomy was always combined with internal fixation and bone cement. With the exception of six patients (5 early deaths), all patients were able to walk after surgery. The Karnofsky index was improved significantly from 35 to 66%. The longest survival time was found in breast carcinomas and myelomas. Preoperative radiological embolization was a keystone in the treatment. Indication for surgery in spinal metastases is critical and needs an interdisciplinary approach. When the patient is suffering from higher degrees of paresis or even paralysis, he/she is no longer an ideal candidate for the operation. The same applies in the presence of uncontrolled primary tumors and neoplastic disease of the GI tract and the bronchus.

Adult↗
PubMed1997

[Replantation of a leg after hip para-articular amputation].

Of all macroreplantations, reattachment of a leg amputated at the proximal thigh presents the biggest difficulty. A patient with no other injuries was treated in this way. A critical discussion of indications in general, the technique in the acute setting, and reconstructive procedures, including the ilizarov distraction technique, is presented. The outcome after 2.5 years is gratifying. The results obtainable in proximal thigh replantation can justify the risk of potentially lethal general complications.

Amputation, Traumatic↗
PubMed1997

Identification of yeasts and coryneform bacteria from the surface microflora of brick cheeses.

Coryneform bacteria and yeasts of 21 brick cheeses from six German dairies, produced by using undefined ripening cultures, were identified. Arthrobacter nicotianae, Brevibacterium linens, Corynebacterium ammoniagenes, Corynebacterium variabilis and Rhodococcus fascians were found in significant numbers. Out of 148 coryneform isolates 36 could not be identified at the species level. With the exception of a large rennet cheese, the coryneform microflora of rennet and acid cured cheeses were similar, but the cheeses had clearly different yeast populations. Debaryomyces hansenii and Galactomyces geotrichum prevailed in rennet cheeses while Kluyveromyces marxianus and Pichia membranaefaciens were the main species found in acid cured cheese. The dominance of Yarrowia lipolytica probably indicates an improper yeast population, resulting in poor cheese quality. Some of the species identified are potential candidates for designing a defined ripening culture for rennet red smear cheese.

Actinomycetales↗
PubMed1996

Isoelectric focusing of Tamm-Horsfall glycoproteins: a simple tool for recognizing recurrent calcium oxalate renal stone formers.

Tamm-Horsfall glycoproteins (THPs) from healthy probands and a majority of recurrent calcium oxalate renal stone formers reveal different physicochemical properties when analyzed using isoelectric focusing (IEF). The pI values of THPs from healthy probands are approximately 3.5 while THPs from recurrent renal stone formers have pI values of between 4.5 and 6. The two groups of THPs exhibit completely different protein patterns. The differences in IEF analysis allow differentiation between THPs from healthy probands and recurrent calcium oxalate stone formers and may possibly be used as a simple diagnostic method for the recognition of recurrent calcium oxalate renal stone formers.

Adjuvants, Immunologic↗
PubMed1996

Potassium induces multiple steroidogenic enzymes in cultured rat zona glomerulosa cells.

The effects of the extracellular potassium concentration on the expression of four steroidogenic cytochromes P-450 (CYP11B2, CYP11B1, CYP11A1, and CYP21A1) in cultured rat adrenal zona glomerulosa cells were studied by Northern blot analysis. At a potassium concentration of 6.4 mM, the CYP11B2 messenger RNA (mRNA) level and aldosterone production were undetectable. Upon increasing the potassium concentration to 18 mM, CYP11B2 mRNA and aldosterone production became measurable and reached a plateau within 4 days. The high potassium concentration also caused distinct increases in the levels of CYP11B1, CYP11A1, and CYP21A1 mRNA, comparable to those induced by ACTH. ACTH did not affect the CYP11B2 mRNA level at the lower potassium concentration. In zona fasciculata cells, CYP11B2 expression was not inducible by potassium; CYP11B1, CYP11A1, and CYP21A1 mRNA levels increased in response to ACTH, but not in response to a high potassium concentration. According to these results, potassium initiates CYP11B2 biosynthesis most likely at the level of transcription or by increasing mRNA stability. Potassium also has a zona glomerulosa-specific inductive effect on three other steroidogenic enzymes.

Adrenal Cortex Hormones↗
PubMed1994

Anatomy and clinical use of posterior interosseous island forearm flap.

Posterior interosseous island flap of the forearm is still not widely known. It deserves more attention as a useful means of reconstruction on the dorsal side of the hand up to the PIP joints of the fingers. The technique is relatively easy, and the anatomy is quite consistent, as described. Tactile sensation can be re-established, and infection cavities can be filled up. Two cases are illustrated as examples.

Adolescent↗
PubMed1994

Tamm-Horsfall glycoprotein: role in inhibition and promotion of renal calcium oxalate stone formation studied with Fourier-transform infrared spectroscopy.

Tamm-Horsfall glycoprotein (THP) from healthy probands inhibits the precipitation of calcium oxalate, whereas THP from individuals who repeatedly develop calcium oxalate stones has no effect or even promotes precipitation. Using Fourier-transform infrared spectroscopy, we found a structural differentiation between these functionally different THPs: a decisive difference in sialic acid content. Quantitative analysis for sialic acid showed the same results. THP from healthy probands had a high sialic acid content (51 +/- 9 g/kg), whereas THP from recurrent stone formers had a decreased sialic acid content (21 +/- 4 g/kg). This explains the dual role of THP in the precipitation of calcium oxalate and the formation of renal stones and shows the importance of glycosylation in the function of this glycoprotein.

Calcium Oxalate↗
PubMed1994

[Diaphragmatic ruptures: diagnosis--therapy--results, experiences with 64 patients].

From 1963 to 1993, 64 Patients with traumatic lesions of the diaphragm were treated. 58 Patients were admitted after blunt trauma, 6 Patients after a penetrating trauma. 87.5% of the patients were male with an average age of 37 years. In about 80% of cases other severe traumatic lesions were noted in association. With 64% extremities, 50% abdomen, 47% thoracic cavity and 38% head injuries were concomitant lesions. Due to the association with pelvic fractures (45%) a special diagnostic emphasis on diaphragmatic ruptures is recommended. In 83% the left diaphragm is involved. Over the last years the right-sided rupture of the diaphragm were found in an increasing extent from 3.7% up to 26%. Conventional radiography with gastrographin swallow is still the essential diagnostic tool. Hospital mortality (12%) was mainly influenced by complications of concomitant injuries, i.e. cardio-pulmonary insufficiency, Shock and multi-organ-failure.

Abdominal Injuries↗
PubMed1993

[Suture of the anterior cruciate ligament--what is the real value of this method?].

After suture of the anterior cruciate ligament without anatomical augmentation, used as a routine method, 88 patients were followed up after a minimum of 2 years using the Lysholm score and the activity scale. Residual laxity was checked clinically and with the KT-1000. For comparison, 20 patients with chronic symptomatic anterolateral instability were followed up who had been operated on using the Eriksson technique during the same time period. Postoperative treatment was a limited functional approach. The peripheral ligament structures were treated following the Hughston and Müller principles. In two-thirds of the knees lateral tenodesis was added. The results of the suture method were disappointing and inferior to the results obtained by the Eriksson technique (trend). The Lysholm score was 77 and 82, respectively. The activity scale was 4.4 (recreational sports) for both groups. The Lachmann sign (KT-1000) in side-to-side comparison (89 N) showed a residual laxity of 1.9 mm and 0.8 mm (average), respectively. In no series was tractopexy of demonstrable advantage. The final conclusion must be that the suture method (with iliotibial tract tenodesis) is not superior to guided conservative treatment. Despite an unfavorable situation preoperatively, the (abandoned) technique of ligament substitution is superior to the suture method (trend). In isokinetic testing dominant knees fare better. In good results, a high hamstrings-quadriceps ratio is typical. The reasons for this are not well-trained hamstrings, but the relative insufficiency of the quadriceps mechanism.

Adolescent↗
PubMed1993

[Fractures of the upper cervical spine--indications for treatment].

Over 2 years, 16 patients with a total of 23 lesions of the craniovertebral junction and the upper cervical spine were treated. Clinically important aspects of diagnosis and classification are outlined. The conditions treated included fractures of the occipital condyle and atlas, dens-axis, and hanged-man fractures. Standard operative treatment, which has to be modified in cases of combined injuries, is presented. Surgery is frequently indicated, especially in elderly and polytraumatized patients. In addition, 2 cases of atlanto-occipital dislocation are described: 1 of these patients survived for 5 days after early atlanto-occipital fusion.

Adult↗
PubMed1992

[Timing of osteosynthesis in pelvic girdle injuries. Advantages and disadvantages of early surgical management].

Eighty-five percent of the patients with pelvic injuries have multiple injuries; the mortality averages 40%. The frequency of laparotomy is about 35%, predominantly because of rupture of the spleen and liver and less often because of rupture of the bladder. New osteosynthesis techniques were developed in an attempt to find better means of local tamponade. After acute cheilotomy, a simple means of ventral internal fixation should always be used. Dorsal stabilization techniques, if required, should seldom be done in the acute setting. Slätis-type external fixation as an isolated mode of stabilization is insufficient in unstable injuries.

Bone Plates↗