PubMed Health⌕ Search

Biomedical subjects

H Seiler

Publications and source records attributed to H Seiler.

At least 37 records · Page 2Linked to original sources
PubMed1990

The yeasts of cheese brines.

A total of 365 yeasts were isolated from the brines of soft, semihard and hard cheeses from different manufacturers. Identification was based on 131 characteristics, primarily employing a method with microtitration plates. Most brines exhibited a characteristic yeast flora. The predominant strains proved to be mainly Debaryomyces hansenii and Candida versatilis. In a few brines Trichosporon beigelii, C. rugosa, C. intermedia, Kluyveromyces marxianus, Saccharomyces sp. and C. tenuis/polymorpha were predominant. Also of importance were C. tropicalis, C. parapsilosis, C. zeylanoides, Issatchenkia orientalis and Geotrichum klebahnii. Not all strains could be clearly identified. Lists of characters are provided for subdividing D. hansenii and T. beigelii. The specificity of the yeast flora of brines is assumed to contribute to the sensory variety of cheeses.

Animals↗
PubMed1989

[Biochemical differentiation of Listeria from cheese].

Listeria spp. isolated from cheese were tested for biochemical characteristics together with reference strains from culture collections. Microtitration plates were used for testing the fermentation patterns. The results were subjected to a numerical cluster analysis based on linkage maps. The variation of the group structures calculating the characteristics with and without the hemolysin reactions is demonstrated. The pathogenic species L. monocytogenes could only be separated from the avirulent species L. innocua by the hemolysin tests. Most of the cheese isolates were identified as L. innocua, some as L. monocytogenes and L. seeligeri. There is a need for an inexpensive commercial test kit to identify the serovars or virulence factors of Listeria spp. in the quality assessment of food. The present study sets up doubts for a sufficiently ensured separation of L. innocua from L. monocytogenes.

Animals↗
PubMed1988

[The Graner capitate interposition arthroplasty in lunate malacia].

In capitate interpositional arthroplasty (Graner II) the necrotic lunate bone is removed and the congruity of the proximal carpal row is restored by interposition of the proximal half of the capitate. The carpal bones are fused except for the scaphotrapezial joint. In this fashion carpal instability can be safely treated. Good results are achieved for both grip strength and relief of pain. Motion is restricted but with a useful range of motion remaining. The arc of remaining motion can be shifted into extension through the use of an opening wedge osteotomy of the capitate. Capitate interpositional arthroplasty without an intercarpal arthrodesis has poor result because of remaining carpal instability.

Adult↗
PubMed1988

[Bicondylar fractures].

Late results after operative treatment of 59 bicondylar fractures were excellent in 38% and fair or poor in 35%. Classic Y- and T-types are rare (30.5%), while comminution and soft tissue lesions (50.7%) are frequent and worsen the prognosis. In about three-fourths of fractures functional rehabilitation is possible after internal fixation according to AO principles. With advanced age and poor bone stock early functional nonoperative treatment is advisable, even though nonunion must be expected to follow in such patients. Closed pinning may be a reasonable alternative in similar situations, and less frequently after open reduction and attempted stable internal fixation. Complications were frequent in this series (25%), deep infection occurring in 4.8%, nonunion in 4.8%, secondary ulnar nerve palsy in 3.5%, and restricted range of motion, in some cases requiring arthrolysis, in 7.1%.

Adult↗
PubMed1987

Stone formation in human kidney.

Microscopic crystals were found by SEM analysis in kidneys of stone forming patients as well as in kidneys of non-stone formers. In most samples calcifications were intratubular in the collecting ducts near the papillary tip. 1-2 micron sized particles lay in a gelatinous substance on the epithelium. The only difference in crystal composition is the higher phosphate content in the kidneys of the non-stone forming group. A correlation between the element concentration of the tissue and the occurrence of microliths is given by the cadmium content of the kidney samples. Both groups showed higher cadmium values in the cortex or medulla when crystals were found in the corresponding papillary samples. Urine supersaturation with respect to calcium oxalate was higher in the stone forming group caused by a higher calcium and oxalate but a lower magnesium excretion.

Calcinosis↗
PubMed1987

[Results of the surgical treatment of distal femoral fractures].

In a follow-up of 97 operated distal femoral fractures good and satisfactory results were found in 64% of the cases. 22% unsatisfactory and 14% poor findings attribute mainly to the comminuted supracondylar fracture types. More than half of the patients were multiple injured. In 83% of the cases the internal fixation was obtained by the use of the condyle buttress plate.

Adult↗
PubMed1987

Transmembrane cation movements during infection of Lactobacillus lactis by bacteriophage LL-H.

Phage LL-H-induced cation (K+, Na+, Mg2+, Ca2+, Cd2+) movements in Lactobacillus lactis bacteria have been studied. The effects of the m.o.i. and external cation concentration have been quantified. LL-H-induced effluxes showed cation specificity: K+ but practically no Mg2+ was lost during LL-H infection at low and moderate m.o.i. (up to about 100). Simultaneously to K+ efflux, divalent cation influxes were observed. These were dependent on the m.o.i. and on concentrations of external divalent cations and were concomitant with phage DNA transport, as concluded from the timing of the first phage-promoted biochemical changes in host cell metabolism and from electron microscopical observations. Host energy was not mobilized with phage-induced divalent cation influx. Several features of divalent cation influxes support the view that divalent cations have to be cotransported into the cell as counterions of LL-H DNA. Phage DNA associated with divalent cations may be the basic feature of the divalent cation dependence of LL-H infection.

Adenosine Triphosphate↗
PubMed1986

[Macro- and microreplantations of the lower leg and foot].

Amputations suitable for replantation in the distal lower leg and foot are seldom. There is no indication if shortening will exceed 5 cm or loss of sensitivity is to be expected. Forefood and toe replantations are to be seen predominantly under aesthetic points of view. Satisfactory results in 10 own cases could be achieved if indications for lower leg replantations were limited to guillotine-type amputations, children and some bilateral lesions. Polytrauma as a rule is not a good indication.

Amputation, Traumatic↗
PubMed1986

Selective medium for isolation of saprophytic coryneform bacteria.

Out of 64 test chemicals for which the toxicity was analysed on pure cultures of Gram-positive and Gram-negative bacteria, nalidixic acid, polymyxin B sulfate, colistin, methyl red, cefotaxim, amikacin, 2-chlorphenol, metronidazole and potassium tellurite were shown to perform best for the selective isolation of coryneform microorganisms; sodium azide, potassium thiocyanate and lithium chloride were not suitable. A new selective agar medium (NPC) for coryneform bacteria containing standard agar, plate count agar or columbia agar together with nalidixic acid (20 mg/l), polymyxin B sulfate (40,000 IU/l) and cycloheximide (100 mg/l) was tested with samples of milk, surface water, waste water and soil. The NPC agar was not inhibitory to micrococci, probably due to their close relatedness with coryneforms. Use of sugar-free nutrient agar (standard agar) restricted growth of Streptococcus faecalis. Pseudomonads, flavobacteria and chromobacteria, which often accounted for 1-5% of the NPC agar-resistant Gram-negative rods, could be distinguished from the Gram-positive bacteria due to differences in colony morphology, since on this medium colony shape is not masked by color reactions. The NPC medium was more effective in eliminating Gram-negative bacteria, bacilli, moulds and yeasts than beta-phenethyl alcohol media and potassium tellurite media.

Actinomycetales↗
PubMed1986

The potassium content of heart muscle following cyclosporine.

The potassium content of cardiac muscle was examined after 9 or 22 days of cyclosporine A administration in normal rats and rats exposed to either renovascular, Goldblatt hypertension or renal ischemia. Cyclosporine A did not result in any alteration in cardiac muscle potassium compared to untreated rats in either group, even though the animals which had been subjected to renal ischemia and cyclosporine A plasma potassium was raised compared to animals subjected just to renal ischemia. The potassium content of cardiac muscle, however, was found to be raised in the hypertrophic hearts of the Goldblatt, renovascular hypertensive animals, both with and without cyclosporine.

Animals↗
PubMed1986

[Biomechanics of the upper ankle joint].

In most cases the ankle joint represents a simple hinge, the unimpaired function of which is dependent on normal play of the distal tibiofibular joint. During flexion and extension of the ankle joint, bending of the fibula is responsible for a substantial part of the mediolateral translational movement of the distal tibiofibular syndesmosis. In comparison with articular body forms under typical stress conditions, ligamentous restraints are of secondary importance. Tensile behaviour of fibular collateral ligaments seems to allow restricted, functional follow-up treatment. In the injury pattern of the ankle joint, the role of the interosseous membrane needs further evaluation. The clinical problems associated with tibiofibular subluxation and synostosis can be explained naturally by the impaired biomechanics of the ankle joint.

Ankle Joint↗
PubMed1985

Fluoridation of drinking water: effects on kidney stone formation.

The influence of fluoride in drinking water on stone formation was studied in animals and in "in vitro" crystallization experiments. In male Wistar rats fluoride inhibits ethylenglycol induced calcification of the kidneys and stone formation. The in vitro results performed in synthetic urine exhibited a dose-dependent delay of crystal growth.

Animals↗
PubMed1985

The oxalate-tolerance-value: a whole urine method to discriminate between calcium oxalate-stoneformers and others.

In undiluted urine portions the oxalate-concentration required for precipitation (= oxalate-tolerance) can be obtained by titration with sodium-oxalate and turbidimetric detection. The corresponding urinary calcium-concentration was measured and the two values plotted against each other. With regard to a standard-curve (measurements in synthetic urine) different behaviour between urines of stone forming patients and others can be observed.

Calcium Oxalate↗
PubMed1985

Renal mercury content in HgCl2-induced acute renal failure in furosemide/saline-protected and nonprotected rats.

Renal mercury content, urinary mercury excretion and renal function were studied in rats with acute renal failure (ARF) induced by subcutaneous injection of 2, 3, 6, or 10 mg/kg HgCl2. Similarly poisoned rats were protected against ARF by continuous intravenous infusion of furosemide and saline. Excellent protection was obtained in rats receiving 2,3, and 6 mg/kg HgCl2, whilst some animals developed moderate azotemia after 10 mg/kg HgCl2. Renal mercury content 48 h after HgCl2 injection did not differ appreciably between protected and nonprotected groups of rats and showed no relation to the dose of HgCl2 injected or to the degree of renal failure. Urinary Hg excretion was variable during the first 24 h after HgCl2 injection and tended to be higher with higher dosage unless the animals became anuric early on. Hg excretion during the second 24 h was independent of dosage, but was comparatively high in functionally well protected rats and low in oliguric animals with severe renal failure. Attempts at detoxication with the potent chelating agent complexon I after 6 mg/kg HgCl2 failed completely: Renal mercury content was similar to that in the other groups of rats and every single rat so treated developed severe anuric renal failure. Although dose-dependent functional injury after HgCl2 may be related to the amount of Hg reaching the kidney during the initial phase, we have to conclude that HgCl2 toxicity is unrelated to the amount of Hg found in renal tissue at 48 h. Furthermore, furosemide/saline protection does not act through increasing urinary Hg excretion or decreasing the amount of toxin accumulating in renal tissue.

Acute Kidney Injury↗
PubMed1985

[Experiences in the use of pedicled muscle flaps in reconstructive accident surgery].

The one year-experience with muscle and musculocutaneous flaps in 16 patients with 18 flaps in different regions of the body is represented. The indications were open fractures with extensive loss of soft tissue, infectious complications after joint-replacement or osteomyelitis, defects after tumor resection or irradiation therapy and decubital ulcers. In 11 patients there was a satisfactory result. Complications with partial loss of the flap resulted from primary transposition in open fractures with marked contusion of the muscle, neglection of the specific vascularisation pattern or irradical necrectomy. Viewing the relatively simple operative techniques with muscle and musculocutaneous transposition or island flaps, free microvascular tissue transfer may be unnecessary in many situations.

Amputation, Traumatic↗