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Biomedical subjects

H Tchórzewski

Publications and source records attributed to H Tchórzewski.

167 records · Page 10Linked to original sources
PubMedDate not supplied

[Mitogenic activity in vitro of lysosomal proteins of peripheral blood granulocytes in patients with multiple sclerosis].

Incorporation of 3H thymidine was investigated in cultures of lymphocytes obtained from peripheral blood of 33 multiple sclerosis patients and 20 healthy controls in presence of PHA or lysosomal proteins from granulocytes. The number of T lymphocytes was determined also in the peripheral blood of these patients. A rise was observed in the mitogenic activity of proteins obtained from lysosomal granules of granulocytes of multiple sclerosis patients at the time of disease exacerbation with a fall of this activity at the time of remission. At the same time a fall of T-lymphocyte count was observed in the blood of multiple sclerosis patients. The correlations between 3H-thymidine incorporation into lymphocytes in vitro, and the mitogenic activity of proteins obtained from lysosomal granules of granulocytes and the count of T lymphocytes are discussed.

Acute Disease↗
PubMedDate not supplied

The effect of chronic exposure to formaldehyde, phenol and organic chlorohydrocarbons on peripheral blood cells and the immune system in humans.

The aim of this study was to evaluate immunological, hematological and biochemical parameters in subjects chronically exposed to inhaled formaldehyde (F), phenol (Ph) and isomers of organic chlorohydrocarbons (Chc) released from Ksylamit. Twenty-two office workers had been exposed for 6 months to vapors of Ksylamit, used for protection of felt plates inside the office building. The concentration of Ksylamit vapor was measured at the end of the 6-month period and the level of Ph and F in the air inside the building was 1.3 mg/m3 and 0.8 mg/m3, respectively. Most of the workers had ailments due to the irritant effect of inhaled Ksylamit, but no remarkable increase in morbidity was found during the 6 months of exposure and in a 3-year follow-up. Morphological parameters of blood and the number of natural killer (NK) cells in the group of exposed subjects were within the range observed in healthy subjects matched for age and sex. The number of T-lymphocytes and NK cell cytotoxicity were significantly decreased. Phytohemagglutinin (PHA)- and alloantigen-induced lymphocyte proliferation was diminished. Some biochemical parameters suggested liver damage, although these parameters did not correlate with the levels of Ph and methanol excreted in the urine. Eight subjects with the highest levels of Ph in the urine had decreased erythrocyte and T-helper lymphocyte numbers, and increased numbers of eosinophils and monocytes. The results obtained prove that the functions of both the immune and hematopoietic systems could be affected by chronic exposure to these toxic substances.

Adult↗
PubMedDate not supplied

The immunoregulatory granulocyte factor (GF). III. Characteristics of target cells for GF.

Granulocyte factor (GF) is a substance secreted selectively from the specific granules of granulocytes in the first minutes of adherence or phagocytosis. GF possesses many immunoregulatory functions. We applied flow cytometric analysis to evaluate the target cells responsible for GF action. Resting lymphocytes did not display binding sites for GF, but 93 +/- 4.6% of the neutrophils isolated from peripheral blood bound GF (mean fluorescence = 122 +/- 11). GF was bound by 26 +/- 7.6% of the lymphocytes from nonstimulated and 42.6 +/- 12% of the lymphocytes from PHA-stimulated cultures, and the mean fluorescence intensity was 14.5 +/- 1 and 54 +/- 10.6, respectively. PHA-stimulated CD8+ cells presented higher expression of binding sites for GF than CD4+ cells. Binding sites for GF were found on 66 +/- 5.1% of activated cells expressing the receptor molecule to IL-2 (CD25) and 50.3 +/- 7.4% of cultured lymphocytes expressing the early activation marker CD69. These values were significantly higher in comparison to the percentage of CD25- and CD69- cells that bound FITC-labeled GF (10.3 +/- 3.3% and 11.3 +/- 3.5%, respectively). The binding was specific, and preincubation of the cells with GF almost completely abolished FITC-labeled GF binding. It has also been shown that GF binds to lymphocytes via molecules other than CD16. High-performance liquid chromatography (HPLC) with fractionated GF showed three separate fractions and two of them had GF-like activity, as demonstrated in the mixed lymphocyte reaction (MLR).

Antigens, CD↗