Biomedical subjects

H Winkelmann

Publications and source records attributed to H Winkelmann.

At least 55 records · Page 3Linked to original sources

PubMed1978

Digitonin induced alterations of the erythrocyte membrane as visible by freeze-fracturing.

Treatment of erythrocytes by low concentrations of digitonin results in the formation of elongated, bulged membrane areas free from particles and intramembraneous tubular structures emerged from these domains. At higher concentrations the tubular structures are present also outside of the membrane. A second, not bulged type of particle-free areas is more or less rounded, but mostly of rectangular shape. The rate of this type of domains increases with the concentration of digitonin. Breaks in these areas lead to a formation of sheets which results in a breakdown of the membrane structure finally into a brittle mass of many small sheets lying irregularly one upon the other. Already during the first steps of this membrane breakdown hemolysis takes place. Cross-linking of the proteins with glutaraldehyde does not entirely block up the membrane alterations. In fixed erythrocytes the formation fo elongated domains is restricted, no tubular structures are present and no dislocations of particles can be observed. Nevertheless smooth sheets localized also outside of the membrane are formed. However the investigated ghosts are not stabilized by glutaraldehyde against the effects of digitonin. Particle dislocations as well as all the other membrane alterations are present. The implications of the obtained results are: 1. The elongated domains and the tubular structures are probably not digitonin-cholesterol-complexes. 2. The formation of crystalline regions by digitonin-cholesterol-complexes destroys the membrane structure.

Digitonin↗

PubMed1978

[Angioblastomas, their special forms and rare locations].

Sorting over of our angioblastoma cases occurring in the period from 1954 to 1977 was carried out under the aspect of special forms and rare localisations. Among a total of 93 angioblastomas in 66 patients, there were 81 infratentorial (60 patients), 5 supratentorial (5 patients), and 7 spinal (4 patients) angioblastomas. For 8 of the infratentorial angioblastomas a participation of the brain stem was found; their prognosis was unfavourable and all these patients died. Angioblastomas occurring in a multiple manner are also involving problems unless they are restricted to the cerebellar spheres and the vermis of the cerebellum. In this group there were 37 angioblastomas occurring in 10 patients with an increased familial occurrence in four cases and a systematic affection with angiomatosis retinae in five patients. Four of these patients also died. The large number of 5 angioblastomas of the cerebrum has induced us to study these cases particularly critically. There was a good prognosis for all these cases; they all were solitary tumours (2 solid tumours and 3 typical large-syctic angioblastomas). The prognosis of the spinal angioblastomas of which 7 were found in 4 patients, is dependent upon location and the question of the systematised affection. Remarkably large is the number of young angioblastoma carriers. All 5 cerebral angioblastomas and 6 cerebellar angioblastomas were found in patients below the age of 18. From these findings conclusions can be drawn with regard to an optimisation of an early detection and the exact angiographic clarificatior in order to avoid unnecessary losses due to transfers being carried out too late and, on the other hand, to sort out inoperable findings.

Adolescent↗

PubMed1977

[Acute disease pictures of cerebrovascular origin in childhood].

To summarize it can be said that an analysis of 1080 cerebral angiographies of children and juveniles up to 16 years of age and an examination of clinical results obtained for a stock of unsifted pediatric and neurosurgical patients allowed as many as 145 cases of vascular processes to be detected in children and juveniles. This number appears to be relatively large even if we consider that we are concerned here with a selected patient stock of a supraregional specialized clinic. If we also consider that the number of angiographies presently performed in cases of vascular disease processes is still comparatively small and that patients have to wait indiscussibly long times before being finally angiographed, then it is obvious that many a vascular disease process still goes undetected or is either misinterpreted or made clear at too late a time. It is here that much remains to be done in order to provide the necessary material basis, for what we still lack is the necessary number of costly diagnostic facilities with highly qualified diagnosticians as well as the necessary follow-up facilities such as, for example, neurosurgical, vasculosurgical, and intensive care units. We therefore welcome recent improvements in this direction, especially since the realization of a large-scale project would make possible an optimum diagnosis and treatment of vascular disease processes in both children and juveniles.

Acute Disease↗

PubMed1977

[Experiences from the conception of a complex-diagnostic neuroradiologic department integrated into a neurosurgical clinic (model of a novel functional and structural solution)].

For planning and designing the reconstruction of an superannuated radiological department of a neurosurgical supra-regional hospital the following requirements had to be taken into account: 1. Complex-diagnostic working style of a neuroradiological department integrated in the hospital complex. 2. Ensuring of a comprehensive operative-technical examination programme by appropriately equipped work places. 3. The aim to improve the working conditions of the personnel and the medical care of the population. The ideal solution was achieved by an extension of the neurosurgical clinic in the form of a diagnostic tract comprising neurosurgical O.P.D. and an after-care department, electro-diagnostic and neuroradiological departments. In the centre of the novel solution there is the function tract of the neuraradiological department in which the complex building method enables a clear division into main zones (patients zone, function zone, personnel zone). It ensures economical working for the benefit of both patients and personnel. Charts showing the sequence of operations and graphic representations of the constructional solution as well as recommendations for equipment standards for the work places illustrate the project, which is certainly suitable for serving as an internation model of similar special establishments of public health services.

Hospital Departments↗

PubMed1976