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Biomedical subjects

H Wirth

Publications and source records attributed to H Wirth.

At least 55 records · Page 3Linked to original sources
PubMed1982

Diurnal fluctuations of cell kinetic parameters in the epidermis and the sebaceous gland of the hamster ear.

By using the colcemid method, the presence of a diurnal variation for the mitosis index was confirmed, showing a definite minimum during the night in the sebaceous gland of the Syrian hamster ear. Furthermore, the number of all labelled cells and the quotient "single-labelled 14C-cells + double-labelled cells/single-labelled 3H-cells" determined with double-labelling autoradiography ([3H] and [14C]thymidine) seem to be subject to rhythmic fluctuations, although statistical support for this observation is lacking. The parallelism of the rhythmic variations in the epidermis and in the sebaceous glands is striking.

Animals↗
PubMed1982

[Generalized eruptive histiocytoma].

A 52-year-old male patient with generalized eruptive histiocytoma persisting for about 20 years is described. The clinical picture is characterized by red-brownish, round to oval nodules histologically consisting mainly of histiocytes. The ultrastructure of these skin lesions shows histiocytic cells at various stages of differentiation with different degree of lipid storage. The generalized eruptive histiocytoma are separated from multicentric reticulohistiocytosis. The significant differences refer to the clinical picture, in particular the organ manifestations associated with the skin lesions. The histological and ultrastructural features of the generalized eruptive histiocytoma differ from this differential diagnosis for lack of giant cells and of interdigitations between the neighbouring cells. The relation between generalized eruptive histiocytoma and the multinodular reticulohistiocytosis is discussed.

Adult↗
PubMed1981

[Oral zinc therapy and sebaceous gland secretion (author's transl)].

Fifteen male Syrian hamsters were treated for 10 days with 3 x 20 mg zinc sulfate (corresponding to 13,5 mg elementar Zn/d) by means of a stomach tube. Fifteen control animals remained untreated. After treatment the size of sebaceous glands was measured with th plate integration method and the mitotic rate was determined with the colchicine method in both treated and control animals. Zinc therapy significantly increases the size of the sebaceous glands, as well as the cell proliferation in the sebaceous glands.

Administration, Oral↗
PubMed1981

On the influence of X-ray irradiation and photochemotherapy with 8-methoxypsoralen on the sebaceous gland of the Syrian hamster ear.

30 male Syrian hamsters were X-ray irradiated on 1 ear with 500 rad (Dermopan 2; setting 3; tissue half value thickness, 7.5 mm). 30 other animals were treated locally on one ear with an emulsion containing 0.15% 8-methoxypsoralen and then with UV A (10 treatments in 23 days; total UV A dose, 5.0 J/cm20. The other ear served as control. An in vivo double-labelling autoradiography with 3H- and 14C-thymidine was carried out on half of the animals of each group. The other half was administered 3H-thymidine and the number of labelled cells still in contact with the basal lamina of the sebaceous gland after 6 days was determined. The effects of X-ray irradiation and photochemotherapy are identical. Cell proliferation in the sebaceous gland was reduced, the length of the S phase was shortened, and the number of marked cells still in contact with the basal lamina after 6 days was decreased.

Animals↗
PubMed1981

Is sebosuppression by cimetidine an antiandrogenic effect?

By using the Syrian hamster ear model it is shown that cimetidine inhibits cell proliferation in the sebaceous gland. Furthermore, the S phase of sebaceous gland cells is lengthened and the number of labelled cells still in contact with the basal lamina of the sebaceous gland 6 days after isotope application related to the total number of labelled sebaceous gland cells is reduced. The effect of cimetidine is absolutely identical with that of cyproterone acetate, but is quite different from that of the nonhormonal sebosuppressive agent benzoyl peroxide.

Androgen Antagonists↗
PubMed1981

Comparative investigations on the effect of 5-fluorouracil on the epidermis and the skin appendages.

Groups of 15 guinea pigs and 10 Syrian hamsters were treated with 5-fluorouracil (Fluoro-uracil "Roche") for 8, 22, and 36 days, respectively. 15 guinea pigs and 10 Syrian hamsters remained untreated. At the end of the experiment the hair root status of the guinea pig and the 3H-thymidine labelling index of the sebaceous glands and epidermis on the Syrian hamsters were determined. 5-Fluorouracil leads to a gradual reduction of both the 3H-thymidine labelling index in the epidermis and the sebaceous glands and the amount of anagenetic hair. The effect is slow at the onset, gradually increases, and is still increasing after 3 weeks of treatment. The reduction in the number of labelled cells is significantly larger in the epidermis than the sebaceous glands.

Animals↗
PubMed1980

Influence of cyproterone acetate and estradiol on cell kinetics in the sebaceous gland of the golden hamster ear.

The cell kinetics of the sebaceous gland of the hamster ear were analysed in untreated animals, in animals treated with cyproterone acetate, and in animals treated with estradiol. Both active substances lead to a reduction in the size of the sebaceous gland (measurement with the integration plate), to a reduction of mitoses (colcemide method), to a reduction in the 3H-thymidine labelling index, to a prolongation of the S-phase (double labelling technique with 3H- and 14C-thymidine), and to a decrease in the number of cells labelled with 3H-thymidine which remain in contact with the basal lamina of the sebaceous gland 6 days after application of the isotope. The results indicate that, like cyproterone acetate, estradiol reduces the effect of androgens on cell kinetics in the sebaceous gland under the test conditions chosen, even if in an entirely different way.

Androgen Antagonists↗
PubMed1980

On the influence of topically applied drugs on cell kinetics in the sebaceous gland. Investigations with the example of selenium disulfide in the Syrian hamster ear.

60 male Syrian hamsters were treated on one ear with 2.5% selenium disulfide in a surfactant base and on the other ear with the same surfactant base. After treatment 15 animals were investigated by 3H-thymidine autoradiography, 15 animals by the colcemid method and 15 animals by the in vivo double labelling technique with 3H- and 14C-thymidine. In 15 animals the porportion of labeled cells was determined which still had contact with the basal lamina in the sebaceous gland 6 days after 3H-thymidine injection. Besides an increase in sebaceous gland size and in cell proliferation, a shortening of the S phase and a reduction of the labeled sebaceous gland cells which still have contact with the basal lamina of the sebaceous gland 6 days after 3H - thymidine injection were determined.

Animals↗
PubMed1980

[Congenital paramedian lip fistulas].

Congenitale fistulae of the lower lip are mainly an aesthetic problem for the affected patients. Although the anomaly may be inherited as an isolated symptom for several generations, it is also indicative of a cleft palate or related syndromes. Since the possibility of additional severe malformations exists for the offspring genetic counseling of patients with fistulae of the lower lip should be considered.

Abnormalities, Multiple↗
PubMed1980

[Telogen effluvium following application of selenium disulfide in the guinea pig].

A standard surfactant solution was applied to one side of the back in 29 male guinea pigs and the same surfactant solution with an addition of 2.5% selenium disulfide to the other side. The application was carried out a total of six times at three-day intervals. The detergent was not washed away to provide an intense exposure to the active substance. Investigations of the hair root status before and after treatment showed significant increase in telogen hair. The present results of animal experiments cannot be transferred without reservations to conventional application in man. However, they may explain isolated observations of diffuse hair loss after treatment with selenium disulfide if one presupposes an especially intense penetration of active substance in these cases.

Animals↗