Biomedical subjects

H Zhang

Publications and source records attributed to H Zhang.

At least 721 records · Page 40Linked to original sources

PubMed2000

[Measurement of gastric emptying with Xianshizi in patients with resected esophageal cancer].

OBJECTIVE: To determine gastric emptying radiological in patients with esophageal cancer and to obtain a similar effect produced by radionuclide study. METHODS: A new product-Xianshizi (XSZ) made of the mixture of barium and silica gel was used quantitatively to evaluate gastric emptying. 17 patients with resected esophageal cancer before and after operation, and 7 healthy volunteers were studied. RESULTS: Preoperative patients had obviously delayed gastric emptying compared with the control group (P < 0.01). Postoperative patients had more delayed gastric emptying compared with themselves (P < 0.05). CONCLUSIONS: Postoperative gastroparesis is related to delayed gastric emptying. The results of this study were identical with those obtained by scintigraphy. Compared with isotope studies, X-ray exam after taking meal composed of XSZ may eventually contribute to detecting delayed gastric emptying quantitatively.

Adult↗

PubMed2000

[An occlusive evaluation proposal to intra-saccular embolization of intracranial aneurysm].

OBJECTIVE: To explore the imaging criteria for evaluating intracranial aneurysm embolization. METHODS: Angiographic occlusive criteria for intracranial aneurysm embolization (aneurysm not opcified angiographically after embolization, 100% occlusion; a little part of aneurysm neck residual, 95%; neck residual, 90%; neck and a little part of aneurysm cavity residual, 80%; some cavity residual, < 80%) were used by 6 specialists to evaluate 121 aneurysms embolized with MDS (mechanical detachable spiral) and/or GDC (guglielmi detachable coil) from March 1995 to July 1999. The rationality, feasibility and limitation of the criteria were discussed. RESULTS: Among the 121 aneurysms, 100% occlusion was reached in 53 aneurysms (43.8%), 95% in 27 (22.3%), 90% in 16 (13.2%), 80% in 15 (12.4%), and less than 80% in 10 (8.3%) respectively. Good accordance was obtained among different doctors in the occlusive evaluation of aneurysm embolization. CONCLUSIONS: The suggesting criteria are simple and feasible in clinical practice, although the effect of imaging follow-up of embolized aneurysm is unknown and the numerical value of occlusive percentage is arbitrary.

Adult↗

PubMed2000

[Application of extracardiac conduit total cavopulmonary anastomosis].

OBJECTIVE: To evaluate the value of application of extracardiac conduit total cavopulmonary anastomosis (TCPA) in complex heart disease. METHODS: From June 1998 to August 1999, 10 patients with functional univentricular complex congenital heart diseases received TCPA. Eight patients were male and 2 female with a mean age of (9.0 +/- 5.6) years (from 2.5 to 21 years) and a mean weight of (25 +/- 14) kg (from 11 to 45 kg). Associated heart anomalies were TGA, PS or ASD. All surgical procedures were performed under general anesthesia and hypothermia. RESULTS: No operative and postoperative deaths and all patients were followed up for 6 - 18 months. All patients were clinically asymptomatic. There was no increase of systemic venous pressure. EKG revealed no arrhythmias. The cardiac function was in NYHA class I-II. CONCLUSIONS: Extracardiac conduit TCPA is a simple procedure and it is superior to others.

Adolescent↗

PubMed2000

[Portal anticoagulation in preventing thrombosis after porta-azygous devascularization for portal hypertension].

OBJECTIVE: To study the prevention method for thrombosis after porta-azygous devascularization for portal hypertension. METHODS: 71 patients with portal hypertension due to cirrhosis were divided into group A (36 patients) and group B (35 patients). In group A, a anticoagulation tube was inserted via splenic vein branch during shunt and anticoagulation therapy was given after operation. RESULTS: All the patients received color Doppler examination 3 months after operation. No thrombosis occurred in the group A and thrombosis occurred in 13 patients (37.14%) in the group B. CONCLUSION: Portal anticoagulation therapy is the effective in preventing portal thrombosis after porta-azygous devascularization for portal hypertension.

Administration, Oral↗

PubMed2000

[Anterior interbody autografting and internal fixation for primary surgical management of thoracolumbar spinal tuberculosis].

OBJECTIVES: To verify the importance of early reconstruction of spinal stability and to evaluate the safety of anterior instrumentation in surgical management of spinal tuberculosis. METHODS: Eleven cases of thoracolumbar spinal tuberculosis were treated with primary anterior debridement, interbody autografting and Z-plate fixation from October 1997 to January 1999. RESULTS: All the 11 cases healed without any recurrence after follow-up for an average of 16 months. Spinal fusion occurred in 3.8 months on average after surgery. 18 degrees of kyphosis correction was achieved with no associated complication. CONCLUSIONS: Early reconstruction of spinal stability plays an important role in surgical management of spinal tuberculosis, and it is safe to implant anterior titanium spinal instrument in tuberculosis foci.

Adolescent↗

PubMed2000

[Point mutations of N-ras oncogene and abnormal expression of rasp21, p53 proteins in orbital rhabdomyosarcoma].

OBJECTIVE: The point mutations of N-ras oncogene and the abnormal expression of rasp21 and p53 proteins in orbital rhabdomyosarcoma (RMS) were analyzed in order to explore the role of oncogene mutations in the pathogenesis of orbital RMS and its relation to patients' prognosis. METHOD: 21 human orbital RMS tissues were analyzed by dot-blot hybridization and immunohistochemistry using synthetic specific oligonucleotide probes and mutant rasp21, p53 monoclonal antibodies. RESULTS: The second base mutation of codon 12 of the N-ras oncogene was found in 4 patients. The third base mutation of codon 61 was found in 5 patients. The mutant rate was 33.3%. By immunohistochemistry analysis, the overexpression of rasp21 and p53 protein level was found in 42.9% and 76.2% respectively. The prognosis of patients with overexpression of ras p21, p53 was worse than that of normal expression (P < 0.01). CONCLUSION: The mutants of ras and p53 oncogene play important roles in the pathogenesis of orbital RMS, and the abnormal expression of their protein products is related to patients' prognosis.

Adolescent↗

PubMed2000

[An ultrastructural observation on rat retina after photoreceptor cell implantation].

OBJECTIVE: To further study the retinal neuronal signal. METHODS: The Wistar/RCS (RCS rats are rats with hereditary photoreceptor degeneration) rats were respectively as donors/acceptors, and the retinal pathway was reconstructed with the technique of pure photoreceptor transplantation. The photoreceptor layer of the retina was obtained with the technique of retinal whittle by manual method or excimer laser. The specimens were got separately at 2 weeks and 1 month after the transplantation and studied under the light and transmission electron microscopes. RESULTS: Most transplanted photoreceptors with physical poles were lined up regularly between the retinal pigment epithelium (RPE) and inner nuclear layer. It was shown that in the new outer plexiform layer the relatively integral synapse and its interconnection were seen. CONCLUSION: The retinal neuronal pathway can be reconstructed by retinal transplantation.

Animals↗

PubMed2000

[The susceptible alleles on HLA-DRB 1 of type I diabetes in children in Harbin].

OBJECTIVE: To evaluate association between type I diabetes in children and HLA-DRB1 alleles in Harbin. METHODS: Case-control method was employed, and the PCR-SSP technique was used to identify HLA-DRB1 alleles. RESULTS: The frequencies of DRB1 * 0301 and DR3/4 heterozygosity were significantly higher but the frequency of DR2 was significantly lower in cases than that in controls. In DR4 subtypes, the frequency of DRB1 * 0405 was higher and the frequency of DRB1 * 0406 was lower in cases than that in the controls. The difference between cases and controls in DRB1 * 0901 was not significant. CONCLUSION: DRB1 * 0301 allele and DR3/4 heterozygosity were the important susceptibility and heterozygosity genes while DR2 was the most important protective gene in Harbin. The DR4 possessed double-tendency. DRB1 * 0405 conferred susceptibility and DRB1 * 0406 conferred little protection. The susceptibility conferred by DRB1 * 0901 was not certain in type I diabetes in childhood in China. This study provided useful genetic data on the study of etiology of type I diabetes mellitus.

Alleles↗

PubMed2000

[Cytochrome P450IA1 and the genetic susceptibility to esophageal carcinoma].

OBJECTIVES: To explore the susceptibility to esophageal carcinoma (EC) in different population with different cytochrome P450 IA1 (CYPIA1) genotype, and study the synergistic effects between CYPIA1 and smoking. METHODS: A case-control study (case = 111, control = 114) was used to compare the frequency of CYPIA1 genotype between EC and controls with a PCR method. RESULTS: The distribution of three genotypes of CYPIA1 in EC and controls was significantly different (chi(2) = 19.35, P < 0.01). There was a synergistic effect between the mutation of CYPIA1 4,889 site and smoking (SIA = 1.56), and a significant dose-response effect was observed. The risk of individual whose CYPIA1 4,889 site is mutated was 3 times higher than those not mutated. CONCLUSIONS: The mutation of CYPIA1 ,89 site maybe one of important host susceptible factors of EC, and the risk would increase significantly in smokers.

Aged↗

PubMed2000

[Studies on algae and microcystin pollution in source water of Yellow River in Zhengzhou city].

OBJECTIVE: To study the law of development of pollution with algae and microcystin (MC) in source water of Yellow River in Zhengzhou city. METHODS: Algae and MC were detected continuously for source water samples collected from B and S Water Works of Zhengzhou in 1998. Microcystin was determined with high-sensitive ELISA. RESULTS: Monthly average density of algae was 1,439 x 10(4)/L in source water of B Water Works of Zhengzhou, with peak algae density of 4,762 x 10(4)/L in August, and that of S Water Works was 1,071 x 10(4)/L. Blue-algae of higher toxicity has become dominant species in source water from S water Works. Detection rate of microcystin was 70.83% in 24 samples collected from the two water works. CONCLUSION: According to Carlson's trophic state index (TSI), as well as other indices, such as total nitrogen, total phosphorus, chemical oxygen demands (COD) and algae density in water, source water in Zhengzhou has presented characteristics of eutrophication.

Bacterial Toxins↗

PubMed2000

[Lung injury caused by passive smoking and its effects on cytokines in rats].

OBJECTIVE: A rat model with chronic bronchitis was replicated by passive inhalation of cigarette smoking fume to study its long-term effects on lung injury and nitric oxide (NO), interluekin-6 (IL-6), interleukin-8 (IL-8). METHODS: Levels of nitrogen dioxide (NO(2)) and nitrogen trioxide (NO(3)) were measured with spectrophotometry in rats indicating their level of nitric oxide (NO). Levels of IL-6 and IL-8 were determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: Levels of NO in serum, bronchial alveolar lavage fluid (BALF) and lung tissue in the smoking group were significantly lower than those in the normal controls (P < 0.01). But, levels of IL-6 and IL-8 were higher in the smoking group than those in the controls. CONCLUSION: Long-term passive smoking could cause injury of lung tissue to certain extent, reduction in secretion of NO in endothelial cells and damage to pulmonary vessels.

Animals↗

PubMed2000

[Genotyping of hepatitis C virus and homology analysis of its core gene sequences isolated from different geological regions].

OBJECTIVE: To study the structure of its genotypes and gene variation of hepatitis C virus (HCV) isolated from different geological regions. METHODS: Serum specimens were collected from patients with HCV infection of Henan and Hebei Provinces of China, as well as of Sweden and Germany. HCV RNA in their serum was detected by retro-transcription polymerase chain reaction (RT-PCR) and genotyped. The core gene of 11 HCV isolates were sequenced and their homology was analyzed for different geographic regions with software. RESULTS: Structure of HCV genotypes, isolated from two provinces of China, and from Sweden and Germany, was not identical. The homology of their core gene sequences of the same HCV genotype isolated from the same region was higher than that from different regions, and subtypes 1b and 2a were found in serum collected from China and subtypes 1a, 2c and 3a in serum from Sweden and Germany. CONCLUSION: Structure of HCV genotypes associated with their geographic distribution and difference in sequences of the same HCV genotype also associated with their geographic regions.

Base Sequence↗

PubMed2000

[Effects of methyl tertiary butyl ether on cell cycle and cell apoptosis].

OBJECTIVE: To explore the effects of the new gasoline additive, methyl tertiary butyl ether (MTBE) on cell cycle and cell apoptosis. METHODS: Flow cytometry was used to evaluate the effect of MTBE (1, 2, 4 microl/ml, 24 h) on NIH/3T3 cell cycles; and the effect of MTBE on Hela cell apoptosis was evaluated by detecting cell survival using crystal violet staining. RESULTS: Flow cytometry showed that MTBE could change NIH/3T3 cell cycles, decrease the number of cells in S stage, and arrest cells at G(2) + M stage. The results suggested that MTBE could affect NIH/3T3 cell cycles and induce cell proliferation. This situation existed 48 hours after the treatment, and cell cycles came back normal 96 hours after the treatment. By detecting cell survival using crystal violet staining, we found that MTBE could inhibit the apoptosis of Hela cells which was induced by tumor necrosis factor (TNF)alpha and cycloheximide. CONCLUSION: MTBE's carcinogenicity to animals may relate to induction of cell proliferation and inhibition of cell apoptosis.

3T3 Cells↗

PubMed2000

[Inhibition of monocytes adhesion to the intima of arterial wall by local expression of antisense monocyte chemotactic protein-1].

OBJECTIVE: To study the mechanism of monocyte recruitment in atherogenesis and to clarify the effect of monocyte chemotactic protein-1 (MCP-1) in this process. METHODS: Femoral arteries isolated from the rabbits which had been fed with a high cholesterol diet and locally perfused with MM-LDL within the artery beforehand, were used as the models. Antisense MCP-1cDNA was transferred into the arterial wall by injecting recombinant LNCX-anti-MCP-1/liposomal complex in the femoral sheath and the periarterial tissue. RESULTS: Expression of antisense MCP-1 mediated by recombinant LNCX plasmid/lipsomal complex gene transfer enabled to inhibit MCP-1 gene expression and adhesion of monocyte to the intima. CONCLUSION: MCP-1 plays an important role on the recruitment of monocytes in the arterial wall, which provides a potential clue in developing a gene therapy project for the prevention and treatment of atherogenesis.

Animals↗

PubMed2000

[Study on the needle biopsies of 100 prostatic adenocarcinoma].

OBJECTIVE: To study the morphological diagnostic criteria of prostatic adenocarcinoma in needle biopsy. METHODS: 250 cases of prostatic needle biopsy including 100 cases of adenocarcinoma were observed retrospectively. The diagnostic significance of 20 parameters of morphological criteria including the architectural changes, cytologic features, invasion and some additional histologic features (intraepithelial neoplasm, intraluminal crystelloid body, intraluminal basophilic mucin secretion, collagen nodule and coagulative necrosis) were analyzed. RESULTS: All 20 parameters of morphological criteria had practical significance in pathologic diagnosis, the most significant ones being architectural changes, prominent nucleoli, perineural and stromal invasion. CONCLUSION: The diagnosis of prostatic adenocarcinoma is based on a constellation of cytologic and architectural features.

Adenocarcinoma↗

PubMed2000

[The expression of connexin 43 and desmin in viral myocarditis].

OBJECTIVE: To study the cytobiological basis of constriction dysfunction and arrhythmia in cardiac muscle cells with viral myocarditis. METHODS: The expression of connexin 43 and desmin in cardiac muscle cells of mice with experimental viral myocarditis was determined by immunohistochemistry. RESULTS: In normal mice, connexin 43 and desmin are located in the intercalated disks of cardiac muscle, and the latter also revealed a positive immunoreactivity in the cross striations of sarcomeres. In viral myocarditis, the expression of both became much weakened, can even become negative. CONCLUSION: In myocarditis, expression of connexin 43 and desmin in the involved cardiac muscle cells was inhibited, resulting in dysfunction of gap junctional communication and arrhythmia.

Animals↗

PubMed2000

[Expression and clinical significance of p53 protein and p21(WAF1) in non-small-cell lung carcinoma].

OBJECTIVE: To investigate the relationships between expression of tumor suppressor gene p53 protein, p21(WAF1), clinicopathology and prognosis in human non-small-cell lung carcinoma (NSCLC). METHODS: SP immunohistochemistry performed in all cases. RESULTS: One hundred forty-seven resected NSCLC were examined. There were 63 squamous cell carcinomas, 66 adenocarcinomas, 14 adenosquamous carcinomas and 4 large cell carcinomas. Overall 90 (61.2%) of the 147 cases had positive p53 staining. The positive ratios of p53 overexpression of squamous cell carcinomas, adenocarcinomas, adenosquamous carcinomas and large cell carcinomas were 63.5% (40/63), 57.6% (38/66), 71.4% (10/14) and 50% (2/4) respectively. The average positive ratio of p21(WAF1) expression was 40.1% (59/147) in lung carcinoma. The positive ratios of p21(WAF1) expression of squamous cell carcinomas, adenocarcinomas, adenosquamous carcinomas and large cell carcinomas were 41.3% (26/63), 42.4% (28/66), 28.6% (4/14) and 25% (1/4) respectively. Over expression of p53 protein was associated with prognosis of the patients with adenocarcinomas. The positive ratio of p53 expression in over 3 year survival patients was 75% (21/28) and in less than 3 year survival, 44.7% (17/38) (P < 0.025). The positive expression of p21(WAF1) was related to prognosis of NSCLC. The ratio of 3-year survival (64.4%) in p21(WAF1) positive NSCLC was much higher than in p21(WAF1) negative patients (46.6%) (P < 0.05). Prognosis of patients with p53 positive but p21(WAF1) negative expression was poorer than those with p53 negative and p21(WAF1) positive NSCLC (P < 0.01). CONCLUSION: These findings suggest that over expression of p53 in adenocarcinomas could be used as a prognostic factor for patients. p21(WAF1) is of protein has a definite value in judging the prognosis in NSCLC. Combined expression of p53 and p21(WAF1) can be used as the prognostic markers in NSCLC and are of significance in estimating the prognosis of patients.

Adenocarcinoma↗

PubMed2000

[Influence of a G/A substitution at -75 bp promoter region of apolipoprotein A I gene and wine consumption on the plasma apolipoprotein A I levels in Yi and Han Chinese].

OBJECTIVE: To study the influence of a common polymorphism of the human apolipoprotein A I gene promoter at a position of -75 bp upstream of the transcription start site and wine consumption on plasma lipid levels. METHODS: 742 healthy Yi and Han healthy subjects all above 15 years old to be the total population which was divided into three samples, namely, Yi-farmer sample, Yi-emigrant sample and Han-resident sample for this study. Estimations of plasma lipids and apolipoproteins were carried on through an auto-analyzer. Genetic DNA was prepared from the frozen blood clot using Triton x-100 lysis technique. Amplification of a 432 bp fragment of the apoA I gene promoter was performed via the PCR, followed by restriction digestion, electrophoresis and identification of the genotypes involved. Data analysis was done at last. RESULTS: Four groups of alcohol consumption were defined: the non-drinkers, 1 - 25 g alcohol intake/day, 26 - 75 g alcohol/day and > 75 g alcohol/day. A tendency of a mild persistent elevation of plasma HDL-C and apoA I was noticed corresponding to an increase of the amount of alcohol intake, and with no evidence of threshold effect observed in the samples of both the Yi-farmers and the Han people. Similar phenomenon was obtained in the sample of Yi-emigrants, but no statistical significance. The frequencies of the A allele of all 3 samples were similar. In Han and Yi-emigrant samples, the drinkers with genotypes of GG had a higher plasma HDL-C and apoA I level than that of the non-drinkers with the same genotypes. Drinkers with A allele had a lower plasma HDL-C and apoA I level than that of drinkers without A allele, and the non-drinkers with A allele had a higher levels of apoA I than of non-drinkers with genotypes of GG. It is estimated that 18% of the variability of plasma apoA I level could be explained by the G/A polymorphism in non-drinkers of Yi-emigrants (F = 8.94, P < 0.01). CONCLUSIONS: Current data suggest that a moderate alcohol consumption or a G to A substitution could make a lower incidence of CHD, but the beneficial effect of one will be negated by the other when both factors occur simultaneously. This finding is seemed valuable for a further study on the effect of the environmental factor or genetic factor in effecting the plasma apoA I level afterwards.

Adolescent↗