Biomedical subjects

H Zhang

Publications and source records attributed to H Zhang.

At least 739 records · Page 41Linked to original sources

PubMed2000

[The effects of massive escharectomy on the resuscitation of burn shock in the early stage after burn].

OBJECTIVE: To evaluate the effects of massive escharectomy on the resuscitation of burn shock in the early stage after severe burn. METHODS: Mongrel dog inflicted with 40% TBSA III degree scalding were employed as the model. The dogs were randomly divided into two groups ------ escharectomy (E) and non -- escharectomy (C) groups. All the dogs received balanced salt solution according to Parkland formula from 1 postburn hour (PBH). The dogs in E group underwent escharectomy en masse at 3 PBH with immediate grafting of fresh alloskin. The dynamic indices of hemodynamics, blood rheology, oxygen supply, oxygen consumption and blood lactic acid content were monitored. RESULTS: Severe shock occurred in all the dogs 30 min after burn. The were evident decreased cardiac output (CO), cardiac index (CI) and left ventricular work index (LVWI) in all the dogs. But the viscosity of whole blood and plasma, the erythrocytic agglutination index and plasma lactic acid content increased significantly. The dogs in E group were operated with massive escharectomy at 3 PBH. In this group, CO, CI, blood rheologic indices and plasma lactic acid content were obviously improved when compared with those in the preoperational period and to those in the C group. CONCLUSION: Simple fluid resuscitation couldn't correct burn shock quickly. Massive escharectomy during shock stage was safe, feasible and effective in the correction of burn shock.

Animals↗

PubMed2000

[Study of the expression and function of c-kit receptor on the bone marrow mononuclear cells of the patients with myelodysplastic syndromes].

OBJECTIVE: To determine the expression and function of c-kit receptor on bone marrow mononuclear cells (BMMNC) of the patients with myelodysplastic syndromes. METHODS: c-kit protein (CD(117)) was detected by direct immunofluorescence assay, c-kit mRNA by reverse transcriptive polymerase chain reaction (RT-PCR) and the function of c-kit receptor by cell culture. RESULTS: c-kit protein expression in MDS was significantly higher than that in control [(8.58 +/- 5.28)% vs (3.04 +/- 1.49)%, P < 0.05]. c-kit protein expression in RA patients was significantly lower than that in RAEB/RAEB-t patients [(5.12 +/- 2.13)% vs (10.01 +/- 5.07)%, P < 0.05]. The rate of c-kit protein expression was (32.43 +/- 18.16)% in AML cases transformed from MDS (t-AML). c-kit mRNA expression in MDS was correlated with c-kit protein expression. Addition of interleukin-3 (IL-3), erythropoietin (Epo) and stem cell factor (SCF) to the semi-solid culture of BMMNC showed significant stimulating effects on CFU-GM and BFU-E formation in normal controls, but did not in MDS patients. CONCLUSION: The protein and mRNA expressions of c-kit receptor on BMMNC in MDS are higher than those in normal control, and the function of this receptor in MDS is abnormal.

Adolescent↗

PubMed2000

[Study on clinical and laboratory features of preleukemia patients].

OBJECTIVE: To explore prospective diagnostic criteria for preleukemia. METHODS: A case control study of the discrepancies of clinical and laboratory features between patients with preleukemia and those with chronic aplastic anemia (CAA) or atypical paroxysmal nocturnal hemoglubinuria (a-PNH). RESULTS: There were eight variables of significance: (1) lymphocytoid micromegakaryocytes in marrow; (2) immature granulocytes in peripheral blood; (3) >or= 2% myeloblasts in marrow; (4) positive periodic acid schiff (PAS) staining of nucleated erythrocytes; (5) myeloid differentiation index >or= 1.8; (6) clonal karyotypic abnormalities; (7) negative sister chromatid differentiation; (8) > 4.0 cluster/colony ratio of granulocyte-macrophage colony-forming units (CFU-GM). The following criteria was assigned: A: To meet (1) and at least two of the other seven variables; B: To meet at least four of the eight variables. All of the patients with preleukemia met A or B and none of the patients with CAA or a-PNH did. CONCLUSION: Preleukemia is different from CAA or a-PNH. It has its own clinical and laboratory features, which may be useful for prospective diagnosis.

Adolescent↗

PubMed2000

[Human physiological regulation in the closed suit with no ventilation].

Objective. To study the effects of the Experimental Closed Suit (ECS) with no active ventilation on human physiological functions and its anti-asphyxic role. Method. Five young healthy male subjects, aged 18-21 years, were tested in the ECS with no active ventilation and no oxygen supply (NAVANOS) when the gas regulatory valve switch was placed on A or B position respectively. Result. (1) Under 1 atm air pressure, each subject could endure at least 15 min while they were aware and resting in the ECS with NAVANOS; (2) There was a balancing period or plateau of the oxygen and the carbon dioxide pressure in the vicinity of the mouth in 5 to 7 min after the sealed helmet was closed, and this period lasted to the end of each test; (3) During the 8-10 min balancing period, the oxygen partial pressure in the vicinity of the mouth ranged from 15.0 to 16.4 kpa, which was slightly hypoxic, and the range of the carbon dioxide partial pressure was 5.21 to 6.49 kPa, which was within the CO2 concentration-time curve of human physiological endurance; (4) No significant difference in human physiological parameters was found when the regulatory valve was placed on A or B position. Conclusion. The anti-asphyxic time of ECS with NAVANOS could at least last 15 min under 1 atm air pressure while the subjects were aware and resting.

Adolescent↗

PubMed2000

[Clinical and experimental study on treatment of acute catarrhal otitis media with eryanling oral liquid].

OBJECTIVE: To study the clinical effect and mechanism of Eryanling (EYL) oral liquid in treating acute catarrhal otitis media (ACOM). METHODS: Sixty-eight cases (89 ears) of ACOM in the treated group were treated with EYL and compared with 34 cases (44 ears) in the control group treated with cephalexinum. Experimental study of effect of EYL on immune function in mice and non-suppurative otitis guinea pig was also conducted. RESULTS: The total effective rate of the treated group and the control group was 91.0% and 84.1% respectively, and their rate of curing 80.9% and 70.5% respectively, though the effect in the former was better, statistic analysis showed no significance between them. The effect initiated obviously earlier in the treated group than that in the control group. Results of experimental study suggested that EYL could strengthen the nonspecific immune function, cellular immune function and humoral immune function in mice, and reduce the degree of inflammatory exudation and mucosa swollen in guinea pig. CONCLUSION: EYL has good therapeutic effect in treating ACOM.

Acute Disease↗

PubMed2000

[Experimental study on inhibitory effect of xiaochaihu decoction on duck hepatitis B virus].

OBJECTIVE: To verify the therapeutic effect of Xiaochaihu decoction (XCHD) on chronic hepatitis B, and to prove the rationality of the TCM principle "strengthening the body resistance to eliminate pathogenic factors". METHODS: The inhibitory effects on DHBV of various doses of XCHD and its ingredients (grouped into whole recipe, partial recipe and single Bupleurum) were determined and compared, as well as compared with that of the blank control and of antiviral drug, acyclovir (ACV). RESULTS: Inhibitory effect was shown in all the treated groups. The treatment by whole recipe of XCHD with the dose 20 times that of clinical use showed the optimal effect, the difference in comparing with other treated groups was significant, P < 0.05. The effect of whole recipe was better than that of the partial recipe or single Bupleurum, P < 0.05. Moreover, the effect was rather persistent, no rebound phenomena was observed after withdrawal of medication, while in the ACV treated group, though a better effect conld be obeained, however, the DHBV returned to the level of before treatment after cessation of ACV treatment. CONCLUSION: The therapeutic effect of XCHD might be to supplment body resistance and remove the evil pathogen, strengthen or regular immune function, so XCHD is efficient in treating chronic hepatitis B.

Acyclovir↗

PubMed2000

[GABA inhibits progesterone production of luteal cells of rat ovary].

In the present work we report that GABA inhibits progesterone production and increases the amount of hydroxyl radical in cultured rat luteal cells. The inhibitory effect of GABA on progesterone production may be mediated by adenylate cyclase system and GABA(A) receptor, but not through protein synthesis pathway.

Adenylyl Cyclases↗

PubMed2000

[Pharmacokinetic analysis of lactosaminated human growth hormone and human growth hormone].

This study was undertaken to explore and compare the pharmacokinetic features of lactosaminated human growth hormone(hGH-L) and human growth hormone (hGH). Radioimmunoassay (RIA) technique was used. After mice were intravenously injected with hGH-L, their blood and liver samples were collected and handled. The blood drug concentration and liver drug concentration were determined using RIA technique. Then the pharmacokinetic parameters were obtained by analysing the curves of blood drug concentration-time and liver drug concentration-time. hGH was determinated with the same methods. The results showed that the area under curve (AUC) of blood drug concentration-time curve of hGH-L was 32,686.90, the mean remain time (MRT) of hGH-L in blood was 21.37 minutes, but the AUC of blood drug concentration-time curve of hGH was 36,913.08 and the MRT of hGH in blood was 24.98 minutes. Therefore, in blood, the uptake of hGH-L was less than that of hGH, the removal of hGH-L was faster than the removal of hGH. In liver, for hGH-L, the half-time of distribution (t1/2 alpha) was 1.84 minutes, the half-time of removal(t1/2 beta) was 11.09 minutes, the AUC of liver drug concentration-time curve was 17,621.9; for hGH, the t1/2 alpha was 2.11 minutes, the t1/2 beta was 75.65 minutes, the AUC of liver drug concentration-time curve was 12,148.2. Therefore, in liver, the uptake of hGH-L was more than that of hGH, the distribution and removal of hGH-L were faster than those of hGH. Therefore, the pharmacokinetic features of hGH-L was better than the feature of hGH.

Amino Sugars↗

PubMed2000

[Effects of maxillary growth of Wistar rats with bilateral artificial cleft palate after premaxillary orthopedic treatment on expression of proliferating cell nuclear antigen(PCNA)].

OBJECTIVE: Being based on the principle of Latham's appliance, this experiment is designed to detect activity of osteoblasts in the maxillary sutures of Wistar rats with bilateral artificial cleft palate by immunohistochemistry technique, and evaluate the effects of this appliance on the growth and development of the maxilla. METHODS: The animal models of male infant Wistar rats with bilateral cleft palate were made by removing some palatal bones, splitting a "V" gap of 1.5 cm wide at the line between premaxilla and segments of maxilla. A sort of appliances, which could be fixed in the mouth of Wistar rats with bilateral cleft palate for correcting protrusion premaxilla was constructed basing on the principle of Latham's appliance. Then the diferent pathological changes of osteoblast proliferation between the experimental group and the two controlled groups were examined. The jugomaxillary sutrues, temporomalar sutures and sphenoipalatine suture were harvested 7, 14 days after premaxillar orthopedic treatment, followed by 4% paraformaldehyde fixing about 1 hour, demineralization with 15% EDTA and 0.5% paraformaldehyde for 48 hours, distilled water washing for a night, dimethylbenzene transparant dealing, and paraffin wax embeding. Proliferating Osteoblasts in all these sutures were investigated using immunohiostochemical technique with monoclonal antibodies of proliferating cell nuclear antigen (PCNA). RESULTS: Seven days after orthopedic treatment, no significant difference was observed between the experimental group and the two controlled groups. While after fourteen days, obvious PCNA-positive expression were observed in cells of all these sutures of the experimental group. CONCLUSION: The distribution of proliferating cells and the degree of cell proliferation change after premaxillary orthopedic treatment. And significant cell proliferation is observed in the experimental group, but there are no significant differences between the two controlled groups.

Animals↗

PubMed2000

[Cephalometrical analysis of effects of premaxillary orthopedic treatment on maxillary growth of Wistar rats with bilateral artificial cleft palate].

OBJECTIVE: To measure the differences between the experimental group and the two controlled groups by using the cephalometric radiography technique to study the effects of premaxillary orthopedic treatments on the growth and development of maxilla. METHODS: To make an animal model with bilateral cleft palate by surgery operation: General anesthesia was carried on by injecting 2.5% pentobarbital (25 mg/kg) into abdominal cavity, then almost the same bone of rats' palate were removed from bilateral palate. An appliance, based on the principle of Latham's appliance was made and designed for premaxillary orthopedic treatment. It was fixed in rats' palate using pins under the condition of general anesthesia. After 20 days of orthopedic treatment, the rats were anesthetized by ether, and cephalometric films were taken under self-designed cephalometer and analysed aided by computer. RESULTS: The distances that represented the maxillary position in the posterior-anterior (PA) direction significantly increased (P < 0.05), while the angles that represented the direction of premaxillary growth significantly decreased (P < 0.05) between the experimental group and the two controlled groups. There were no significant difference between the two controlled groups. CONCLUSION: Premaxilla of rats can be effectively moved backward and rotated downward and backward on the PA direction by appliance. The appliance can move the segment palate forward during the premaxillary backword. The appliance can stimulate maxillary growth.

Animals↗

PubMed2000

[Clinical analysis of vocal cord leukoplakia in 32 cases].

OBJECTIVE: To investigate the types and curative effects of the leukoplakia of the vocal cord. METHOD: From 1990 to 1996, 32 patients (38 sides) with leukoplakia of vocal cord were operated in our hospital, postoperative follow-up ranged from 2 to 7 years. RESULT: There were eighteen cases of simple leukoplakia and fourteen cases of leukoplakia with mild, moderate and severe atypical hyperplasia in this study. None of simple leukoplakia had malignant change. But there were 33%, 75% and 75% of cases had malignant change in the leukoplakia with mild, moderate and severe atypical hyperplasia respectively. To surgical methods, there was no significant difference, in the rate of malignant change either by the microlaryngoscopy or thyrotomy. CONCLUSION: Simple leukoplakia can be cured. More attention should be paid to the leukoplakia with any types of atypical hyperplasia during clinical management. The postoperative results were not depend on the surgical methods.

Adult↗

PubMed2000

[Studies on the active constituents of sichuan liang shan rhododendron L. I. Determination of two flavonoids in leaves, extracts and jinjuan liquors].

A reversed-phase HPLC method for the separation and determination of the two flavonoids: quercetin and kaempferol is described. The column was 25 cm x 4.6 mm i.d., packed with Zorbax SB-C18(5 microns). The mobile phase was a mixture of MeOH-H2O (60:40, V/V). The flow rate was 0.7 mL/min. UV detection was performed at 360 nm. External standard was used and the calibration curves showed good linearity over the range of 6.0-100 mg/L, r = 0.9995-0.9998, and detection limits were 3.3-6.2 mg/L. The recoveries were 96.0% and 97.3% for quercetin and kaempferol respectively. The relative standard deviations of the method were 2.1%-4.5%. The method is simple, fast, sensitive and accurate and can be applied to the analysis of quercetin and kaempferol. It provides a scientific basis for industrial production and quality control of Jinjuan liquor preparations for clinical uses.

Antioxidants↗

PubMed2000

[A clinical study on PA-MSHA vaccine used for adjuvant therapy of lymphoma and lung cancer].

The objective of this study was to evaluate the effectiveness and safety of pseudomonas aeruginosa MSHA vaccine (PA-MSHA vaccine) used as an immune modulator. The study was carried out after the design of a non-blind, randomized controlled trial. The malignant lymphoma patients were divided into experiment group (45 cases) and control group (43 cases). Likewise were distributed the lung cancer patients: 44 cases in experiment group and 45 cases in control group. The two experiment groups received chemotherapy + PA-MSHA vaccine. The two control groups were given chemotherapy alone. The results showed that the clinical efficacy rate was 95.56% for the exp. group and 69.77% (P < 0.01) for the control group of the malignant lymphoma cases; the clinical efficacy rate was 59.09% for the exp. group and 42.22% (P < 0.01) for the control group of the lung cancer cases. The infection rate was 17.78% for the exp. group and 37.21% (P < 0.05) for the control group of the malignant lymphoma cases; the infection rate was 15.91% for the exp. group and 40.00% (P < 0.05) for the control group of the lung cancer cases. The comprehensive immune effectiveness rates for the exp. group and control group of the malignant lymphoma cases were 77.78% and 23.26% (P < 0.01) respectively. The comprehensive immune effectiveness rates for the exp. group and control group of the lung cancer cases were 84.09% and 35.56% (P < 0.01) respectively. Adverse reactions were found in 5 cases, of which, one was given medical care while the others automatically recovered. These results indicate that PA-MSHA vaccine, as a new kind of immune modulator, can improve the effectiveness of treatment for tumor with low adverse reaction rate.

Adjuvants, Immunologic↗

PubMed2000

[Isolation of moniliformin-degrading bacterium Ochrobactrum sp. and analysis of its functional properties].

A moniliformin(MON)-degrading bacterium strain, named as Y21-2, was isolated from the mycotoxin-contaminated soil from Heilongjiang Province by the enrichment microculture technique. This strain can grow with MON as its sole carbon and energy source. In the minimal medium with 500 micrograms/mL MON, the number of cells increased from 10(7) to 10(10). Traditional taxonomy, assays of its G + C content and 16S rDNA sequence homology identified Y21-2 as Rhizobiaceae, Ochrobactrum sp. Resting cell suspensions prepared from induced Y21-2 can degrade MON with great speed, which also suggested the existence of enzymes committed to MON degradation in the cell.

Biodegradation, Environmental↗

PubMed2000

[Studies on the trehalose-producing by Colloides CB39].

A bacterium which had the ability of producing sugar produced sugar at low temperature (18 degrees C) was selected from the water of Tianchi in Changbai mountain. The sugar was identified to be trehalose by methods of thin-layer chromatography, reaction of producing osazyone, capillary electrophoresis and infrared spectrum. It was also been found that the trehalose produced by this bacterium (identified to be a new species of Colloides Sp. CB39) was exocrine. At 18 degrees C, its trehalose content in culture solution was 256 mg/g dry weight of the strain. This characteristic is different from that of other strains, which had been proved to have the ability of producing trehalose. then the strain CB39 was induced by ultraviolet in order to abstain mutants which can produce trehalose in high level at usual temperature. The mutant strain 5 that could produce trehalose in high level at usual temperature (25 degrees C) was selected from all the mutant strains. The value of its productivity of trehalose is 416.7 mg/g dry weight of the strain. (At the same temperature, trehalose productivity of mutant strain 5 was eight times of that of the wild type strain CB39).

Bacteria↗

PubMed2000

Alleviation of pre-exposure to low-dose 16O8+ ion on mouse testicular histological damage induced by subsequent high-dose irradiation.

The testes of the B6C3F1 hybrid strain mice were irradiated with 0.05 Gy of 16O8+ ion as the pre-exposure dose (D1), and were then irradiated with 2 Gy of 16O8+ ion as challenging radiation dose (D2) at 4 h after per-exposure. Testicular morphology was observed by light microscope at 35th day after radiation. The results showed that irradiation of mouse testes with 2 Gy of 16O8+ ion significantly impaired, mainly reduction of tubule diameter and decrease or loss of germ cells in various developing stages, especially spermatogenic elements. Pre-exposure to a low-dose (0.05 Gy) of 16O8+ ion significantly alleviated above mentioned damage on testicular morphology induced by subsequent a high-dose (2 Gy) radiation.

Animals↗

PubMed2000

[Cloning and expression of caiDE genes in E. coli BL21(DE3)].

The caiDE genes for carnitine racemase and its relative factor were cloned into pUC18 from the chromosomal DNA of E. coli MC4100 by shot-gun approach and have been verified by sequence analysis. The target plasmid was named as pJX393 and subcloned to form a transcription plasmid pDSW2. After its transformation into BL21(DE3) strain and induction by IPTG, the recombinant strain containing pDSW2 expressed two apparent protein bands on SDS-PAGE, whose molecular weight were 30 kD and 24 kD individually.

Base Sequence↗

PubMed2000

[The construction and application of Streptomyces-E. coli shuttle plasmid pSGLgpp].

The high-copy-number plasmid pSGL1(7.4 kb) was isolated from Streptomyces geobisporous. Its minimal replicon had been determined and sequenced. With the total DNA of S. geobisporus as template, the DNA fragment involving C1027 apoprotein signal peptide encoding sequence (gpp) was amplified by PCR. After this fragment was inserted into the plasmid pSGLN, a derivative plasmid of pSGL1, one new Streptomyces-E. coli shuttle plasmid was obtained namely as pSGLgpp. Using the new vector, we carried out the expression of hsIL-1RI in streptomyces.

DNA, Bacterial↗